Preparation method for whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity

A technology of antibacterial activity and chelate, which is applied in the field of preparation of leech polypeptide zinc chelate, can solve the problems of environmental pollution, protein waste, etc., and achieve the effect of improving chelation rate and high yield
CN105349601AInactive Publication Date: 2016-02-24CHANGSHU INSTITUTE OF TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHANGSHU INSTITUTE OF TECHNOLOGY
Publication Date
2016-02-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method for a whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity. The method comprises the following main steps: (1) degreasing whitmania pigra Whitman tissue; (2) performing enzymolysis on whitmania pigra Whitman protein; (3) separating whitmania pigra Whitman polypeptide; (4) performing chelation reaction on whitmania pigra Whitman polypeptide subjected to enzymolysis and zinc sulfate heptahydrate; and (5) performing alcohol precipitation separating and washing on the chelation reaction product. The prepared whitmania pigra Whitman polypeptide zinc chelate possesses relatively high yield and chelation rate, and oxford-cup-method bacteriostasis detection test proves that the whitmania pigra Whitman polypeptide zinc chelate possesses obvious antibiosis biological activity on escherichia coli and bacillus subtilis, and possesses great application prospect in the field of food and medicines.
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Description

technical field

[0001] The invention relates to the technical field of production of aquatic animal biological products, in particular to a preparation method of leech polypeptide zinc chelate with antibacterial activity. Background technique

[0002] With the development of medical technology in recent years, antibiotics have become one of the medicines that people often use. With the more and more widespread application of antibiotics, a series of problems such as the abuse of antibiotics have emerged, which seriously threaten human life and health. At present, in the medical field all over the world, the irrational use and abuse of antibiotics is very serious, which directly leads to the strong drug resistance of some bacteria and reduces the efficiency of antibiotics.

[0003] In the face of the increasing drug resistance of some bacteria, the most effective and direct way is to find a new type of antibiotic with strong antibacterial ability. Studies have shown that so...

Claims

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