Preparation method and application of small peptide chelated zinc compound

A small peptide chelation and complex technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as stability problems that are not well solved, achieve high chelation rate, improve immunity Good performance and stability

Inactive Publication Date: 2012-12-19
GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The second generation of simple organic salts, although they have good stability, they still have antagonism with some nutrients
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Method used

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  • Preparation method and application of small peptide chelated zinc compound
  • Preparation method and application of small peptide chelated zinc compound
  • Preparation method and application of small peptide chelated zinc compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] The preparation method 1 of small peptide complex comprises the following steps:

[0088] 1) Preparation of culture medium: 100kg soybean meal with a crude protein content of 46%, 100kg rice protein powder with a crude protein content of 60%, 100kg soybean protein concentrate with a crude protein content of 65%, 6kg sucrose, and dilute to 1000L with water , adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;

[0089] 2) Preparation of small peptide fermentation broth: put the Bacillus licheniformis strain cultured in a 32°C constant temperature shaker at 200r / min for 24h into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 7.0, add 3kg of neutral protease (500U / g protein material), and continue...

Embodiment 2

[0094] The preparation method 2 of small peptide complex comprises the following steps:

[0095] 1) Preparation of culture medium: 100kg of soybean meal with a crude protein content of 46%, 100kg of rice protein powder with a crude protein content of 60%, 100kg of soybean protein concentrate with a crude protein content of 65%, 3kg of sucrose, 3kg of glucose, and 2kg of starch , adjust the volume to 1000L with water, adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;

[0096] 2) Preparation of small peptide fermentation broth: put the Bacillus subtilis strain cultured in a 32°C constant temperature shaker at 200r / min for 24 hours into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 6.8, add 2.4kg of ...

Embodiment 3

[0101] The preparation method 3 of small peptide complex comprises the following steps:

[0102] 1) Preparation of culture medium: 100kg of soybean meal with a crude protein content of 46%, 100kg of rice protein powder with a crude protein content of 60%, 100kg of soybean protein concentrate with a crude protein content of 65%, 8kg of glucose, and dilute to 1000L with water , adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;

[0103] 2) Preparation of small peptide fermentation broth: Bacillus natto ( Bacillus natto ) strain solution into the above-mentioned sterilized 1000L culture solution, so that the concentration of bacteria in the culture solution is about 5×10 7 cfu, after culturing at 32°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 6.8, add 3.6kg of neutral protease (600U / g protein raw material), and continue enzymatic hydrolysis at 43°C...

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Abstract

The invention discloses a preparation method and application of a small peptide chelated zinc compound. The preparation method comprises the following steps: preparing a nutrient solution from a protein raw material, a carbon source and water and carrying out sterilization; inoculating bacillus and protease into the sterilized nutrient solution and then carrying out fermentation enzymolysis or enzymolysis fermentation so as to obtain small peptide fermentation liquor; continuing to add a water-soluble zinc salt and carrying out a chelation reaction under the conditions of a reaction temperature of 55 to 65 DEG C and a pH value of 5 to 6; and drying a reactant so as to obtain the small peptide chelated zinc compound. According to the invention, the preparation method for the small peptide chelated zinc compound has a high chelation rate, as high as more than 94%; the prepared small peptide chelated zinc compound has good stability in the stomach of an animal, contains a plurality of bioactive components and has more comprehensive nutrients, so the small peptide chelated zinc compound can be used as a feed additive to supplement trace elements for daily ration and improve immune functions of animals.

Description

technical field [0001] The invention relates to a preparation method and application of a small peptide chelated zinc complex. Background technique [0002] In order to ensure the normal function of each cell, tissue, organ, gland and system, animals need specific trace elements. Trace elements are indispensable nutrients for animals. Adding certain high-quality and high-utility trace elements in the diet is beneficial to animals. Health and maximizing their productive potential are of paramount importance (NRC, 1998; Richards, 2008). [0003] Zinc is a component of many enzymes in animals and an activator of some enzymes. For example, zinc is involved in glucose metabolism, and each insulin molecule contains two zinc atoms, which are closely related to the production, secretion, storage and activity of insulin; zinc is also present in the enzymes involved in the transport of oxygen and carbon dioxide by red blood cells. Zinc is closely related to the synthesis of nucleic ...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23K1/16C12R1/10C12R1/125C12R1/07
Inventor 潘军张伟强王瑞平苏全马春平
Owner GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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