Preparation method and application of small peptide chelated zinc compound
A small peptide chelation and complex technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as stability problems that are not well solved, achieve high chelation rate, improve immunity Good performance and stability
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Embodiment 1
[0087] The preparation method 1 of small peptide complex comprises the following steps:
[0088] 1) Preparation of culture medium: 100kg soybean meal with a crude protein content of 46%, 100kg rice protein powder with a crude protein content of 60%, 100kg soybean protein concentrate with a crude protein content of 65%, 6kg sucrose, and dilute to 1000L with water , adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;
[0089] 2) Preparation of small peptide fermentation broth: put the Bacillus licheniformis strain cultured in a 32°C constant temperature shaker at 200r / min for 24h into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 7.0, add 3kg of neutral protease (500U / g protein material), and continue...
Embodiment 2
[0094] The preparation method 2 of small peptide complex comprises the following steps:
[0095] 1) Preparation of culture medium: 100kg of soybean meal with a crude protein content of 46%, 100kg of rice protein powder with a crude protein content of 60%, 100kg of soybean protein concentrate with a crude protein content of 65%, 3kg of sucrose, 3kg of glucose, and 2kg of starch , adjust the volume to 1000L with water, adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;
[0096] 2) Preparation of small peptide fermentation broth: put the Bacillus subtilis strain cultured in a 32°C constant temperature shaker at 200r / min for 24 hours into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 6.8, add 2.4kg of ...
Embodiment 3
[0101] The preparation method 3 of small peptide complex comprises the following steps:
[0102] 1) Preparation of culture medium: 100kg of soybean meal with a crude protein content of 46%, 100kg of rice protein powder with a crude protein content of 60%, 100kg of soybean protein concentrate with a crude protein content of 65%, 8kg of glucose, and dilute to 1000L with water , adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;
[0103] 2) Preparation of small peptide fermentation broth: Bacillus natto ( Bacillus natto ) strain solution into the above-mentioned sterilized 1000L culture solution, so that the concentration of bacteria in the culture solution is about 5×10 7 cfu, after culturing at 32°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 6.8, add 3.6kg of neutral protease (600U / g protein raw material), and continue enzymatic hydrolysis at 43°C...
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Application Information
- IPC
- C12P21/06; A23K1/16; C12R1/10; C12R1/125; C12R1/07
- Inventors
- 潘军; 张伟强