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Preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium

A technology of peptide chelating calcium and collagen peptides, which is applied in the biological field, can solve the problems of low bioavailability, strong gastric irritation, and low calcium content in soluble products, and achieve high biological potency and high chelation rate , Improve the effect of economic and ecological benefits

Inactive Publication Date: 2019-06-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a comprehensive utilization of collagen polypeptide of wood frog bone and flesh, and to solve the problems that currently commercially available calcium supplement products have strong irritation to the stomach, low bioavailability, and low calcium content in products with good solubility. Provided is a preparation method for chelated calcium of Rana bone meat collagen polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Wash 100g of fresh Rana flesh and blood, place it in a blast drying oven at 60°C for 2.5 hours, put it in a high-speed pulverizer after drying, and pass through a 50-mesh sieve to obtain 86g of Rana flesh and blood powder ;

[0026] (2) Add distilled water to the pulverized wood frog bone meal with a material-to-liquid ratio of 1:10g / ml for enzymolysis. The enzymolysis conditions are set as follows: the amount of enzyme added to trypsin is 38000U / mg, and the enzymolysis time is 4h, the enzymolysis temperature is 45°C, and the pH is 8; after the enzymolysis is completed, inactivate the enzyme at 90°C for 20 minutes; put the enzyme-inactivated polypeptide solution in a high-speed centrifuge at 10,000r / min for 30min, and then obtain the enzymolyzed collagen of Rana flesh and blood Polypeptide liquid 832mL and residue 14.6g.

[0027] (3) Obtain the enzymatically hydrolyzed collagen polypeptide solution of Rana carnata, perform ultrafiltration with a filter membrane wit...

Embodiment 2

[0030] (1) Wash 150g of fresh Rana flesh and blood, place it in a blast drying oven at 55°C and dry for 2.5 hours, put it in a high-speed pulverizer after drying, and pass through a 40-mesh sieve to obtain 122g of Rana flesh and blood powder ;

[0031] (2) Add distilled water to the pulverized wood frog bone meal with a material-to-liquid ratio of 1:12g / ml for enzymolysis. The enzymolysis conditions are set as follows: the amount of enzyme added to trypsin is 30000U / mg, and the enzymolysis time is 4h, the enzymolysis temperature is 45°C, and the pH is 8.2; after the enzymolysis is completed, inactivate the enzyme at 95°C for 15 minutes; place the enzyme-inactivated polypeptide solution in a high-speed centrifuge at 10,000r / min for 30min to obtain enzymolyzed collagen from Rana flesh and blood Polypeptide solution 1372mL and residue 22.6g.

[0032] (3) Obtain the enzymatically hydrolyzed collagen polypeptide solution of Rana carnata, perform ultrafiltration with a filter membr...

Embodiment 3

[0035] (1) Wash 200g of fresh Rana flesh and blood, place it in a blast drying oven at 65°C for 2 hours, put it in a high-speed pulverizer after drying, and pass through a 50-mesh sieve to obtain 133g of Rana flesh and blood powder;

[0036] (2) Add distilled water to the pulverized wood frog bone meal with a material-to-liquid ratio of 1:15g / ml for enzymolysis. The enzymolysis conditions are set as follows: the amount of enzyme added to trypsin is 15000U / mg, and the enzymolysis time is 4h, the enzymolysis temperature is 45°C, and the pH is 7.5; after the enzymolysis is completed, the enzyme is inactivated at 90°C for 20 minutes; the polypeptide solution after the enzymolysis is placed in a high-speed centrifuge at 10,000r / min for 25 minutes, and the enzymolyzed collagen of Rana flesh and blood is obtained Polypeptide solution 1447mL and residue 37.6g.

[0037] (3) Obtain the enzymatically hydrolyzed collagen polypeptide solution of Rana carnata, perform ultrafiltration with a...

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Abstract

The invention relates to a preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium. Wood frog bone and flesh chelated calcium is researched by the aid of a lot of wood frog bone and flesh, bones are reasonably treated, waste of resources is avoided, environmental pressure is reduced, and economic and ecological benefits are improved. The method includes the steps: wood frog bone and flesh powder roughcasting; wood frog bone and flesh powder enzymolysis; enzymolysis liquid ultra-filtration; chelating of enzymolysis liquid and calcium chloride. The preparationmethod has the advantages that polypeptide is prepared by a method of trypsin enzymolysis, small molecular weight polypeptide is prepared by a nano-filtration method, and the collagen polypeptide liquid with small molecular weight is acquired. The collagen polypeptide liquid and calcium ions are chelated by the method to obtain the polypeptide chelated calcium, absorption rate in human bodies is increased, calcium supplement effects are good, the method is simple to operate, chelating can be achieved without freeze-drying preparation powder, the method is high in efficiency, easy to control, and prepared collagen polypeptide chelated calcium is high in chelation rate. The prepared wood frog bone and flesh collagen polypeptide chelated calcium can be used for development of common foods andhealth care products or used for the field of medicines.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of collagen polypeptide chelated calcium, in particular to a preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium. Background technique [0002] The Northeast Rana is a pure wild animal that grows in the three provinces of Heilongjiang, Jilin and Liaoning. It integrates beauty, medicine and food, and is a famous economic frog species in my country. According to incomplete statistics, the annual output of forest frogs in Northeast China is about 1 billion. Most of the uses of forest frogs are to extract forest frog oil, but the oil of forest frogs only accounts for about 15% of the whole frog, and it can only be obtained from Extracted from the middle of the female frog, other parts such as brain, skin, meat and bone are basically treated as feed or waste, which has caused serious waste of resources. The flesh and blood of wood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/165A23L33/18A23L13/40A61K38/01A61P3/14C12P21/06C07K1/34
Inventor 关爽刘宇杭卢静
Owner JILIN UNIV
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