Preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium

Abstract

The invention relates to a preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium. Wood frog bone and flesh chelated calcium is researched by the aid of a lot of wood frog bone and flesh, bones are reasonably treated, waste of resources is avoided, environmental pressure is reduced, and economic and ecological benefits are improved. The method includes the steps: wood frog bone and flesh powder roughcasting; wood frog bone and flesh powder enzymolysis; enzymolysis liquid ultra-filtration; chelating of enzymolysis liquid and calcium chloride. The preparationmethod has the advantages that polypeptide is prepared by a method of trypsin enzymolysis, small molecular weight polypeptide is prepared by a nano-filtration method, and the collagen polypeptide liquid with small molecular weight is acquired. The collagen polypeptide liquid and calcium ions are chelated by the method to obtain the polypeptide chelated calcium, absorption rate in human bodies is increased, calcium supplement effects are good, the method is simple to operate, chelating can be achieved without freeze-drying preparation powder, the method is high in efficiency, easy to control, and prepared collagen polypeptide chelated calcium is high in chelation rate. The prepared wood frog bone and flesh collagen polypeptide chelated calcium can be used for development of common foods andhealth care products or used for the field of medicines.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a preparation method of collagen polypeptide chelated calcium, in particular to a preparation method based on wood frog bone and flesh collagen polypeptide chelated calcium. Background technique

[0002] The Northeast Rana is a pure wild animal that grows in the three provinces of Heilongjiang, Jilin and Liaoning. It integrates beauty, medicine and food, and is a famous economic frog species in my country. According to incomplete statistics, the annual output of forest frogs in Northeast China is about 1 billion. Most of the uses of forest frogs are to extract forest frog oil, but the oil of forest frogs only accounts for about 15% of the whole frog, and it can only be obtained from Extracted from the middle of the female frog, other parts such as brain, skin, meat and bone are basically treated as feed or waste, which has caused serious waste of resources. The flesh and blood of wood...