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Preparation method and application of small peptide chelated manganese compound

A small peptide chelation and complex technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problem of stability problems that are not well resolved, achieve high chelation rate, improve immunity Comprehensive effect of function and nutrition

Inactive Publication Date: 2012-12-19
GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second generation of simple organic salts, although they have good stability, they still have antagonism with some nutrients
However, the stability problem in the rumen or monogastric acid environment has not been well resolved (Liang Jianguang et al., 2008)

Method used

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  • Preparation method and application of small peptide chelated manganese compound
  • Preparation method and application of small peptide chelated manganese compound
  • Preparation method and application of small peptide chelated manganese compound

Examples

Experimental program
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Effect test

preparation example Construction

[0077] 2. Preparation of small peptide chelated manganese and inorganic manganese

[0078] Extraction of total manganese: Weigh about 0.15g of sample into a crucible, place the crucible on a heating plate and heat until the sample is completely carbonized. Transfer the crucible to a muffle furnace that has been preheated at 550°C for 15 minutes and incinerate for 3 hours. After cooling, soak the contents of the crucible with 2ml of water. If there are many carbon particles, dry the crucible on a water bath, then put it into a muffle furnace for ashing for 2 hours, let it cool and add 2ml of water. Add 10 ml (1+1) hydrochloric acid, heat and boil until the contents are almost dry, be sure to avoid splashing the contents during heating. After dissolving the residue by heating with 5 ml (1+1) hydrochloric acid, cool it and quantitatively transfer it to a 100 ml volumetric flask. Take 1ml of the solution to a 100ml volumetric flask and make to volume.

[0079] Determination of ...

Embodiment 1

[0087] The preparation method 1 of small peptide complex comprises the following steps:

[0088] 1) Preparation of culture medium: 100kg soybean meal with a crude protein content of 46%, 100kg rice protein powder with a crude protein content of 60%, 100kg soybean protein concentrate with a crude protein content of 65%, 6kg sucrose, and dilute to 1000L with water , adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;

[0089] 2) Preparation of small peptide fermentation broth: put the Bacillus licheniformis strain cultured in a 32°C constant temperature shaker at 200r / min for 24h into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 7.0, add 3kg of neutral protease (500U / g protein material), and conti...

Embodiment 2

[0094] The preparation method 2 of small peptide complex comprises the following steps:

[0095] 1) Preparation of culture medium: 100kg of soybean meal with a crude protein content of 46%, 100kg of rice protein powder with a crude protein content of 60%, 100kg of soybean protein concentrate with a crude protein content of 65%, 3kg of sucrose, 3kg of glucose, and 2kg of starch , adjust the volume to 1000L with water, adjust the pH value of the reaction solution to 6.8 with hydrochloric acid or sodium hydroxide, and sterilize at 121°C for 18 minutes;

[0096] 2) Preparation of small peptide fermentation broth: put the Bacillus subtilis strain cultured in a 32°C constant temperature shaker at 200r / min for 24 hours into the above-mentioned sterilized 1000L culture broth to make the concentration of bacteria in the culture broth About 5×10 7 cfu / ml, after culturing at 30°C for 24 hours, add hydrochloric acid or sodium hydroxide to adjust the pH of the medium to 6.8, add 2.4kg of ...

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Abstract

The invention discloses a preparation method and application of a small peptide chelated manganese compound. The preparation method comprises the following steps: inoculating bacillus into a nutrient solution, carrying out shake cultivation at a temperature of 30 to 37 DEG C for 18 to 30 h, adding protease and continuing cultivation for 6 to 12 h so as to obtain small peptide fermentation liquor, or adding protease into the nutrient solution, carrying out enzymatic hydrolysis for 6 to 12 h, inoculating bacillus and then carrying out shake cultivation at a temperature of 30 to 37 DEG C for 18 to 30 h so as to obtain the small peptide fermentation liquor; continuing to add a water-soluble manganese salt and carrying out a chelation reaction under the conditions of a temperature of 55 to 65 DEG C and a pH value of 5 to 6; and drying a reactant so as to obtain the small peptide chelated manganese compound. According to the invention, the preparation method for the small peptide chelated manganese compound has a high chelation rate, as high as more than 95%; the prepared small peptide chelated manganese compound has good stability in the stomach of an animal, contains a plurality of bioactive components and has more comprehensive nutrients, so the small peptide chelated manganese compound can be used as a feed additive to supplement trace elements for daily ration and improve immune functions of animals.

Description

technical field [0001] The invention relates to a preparation method and application of a small peptide chelated manganese complex. Background technique [0002] In order to ensure the normal function of each cell, tissue, organ, gland and system, animals need specific trace elements. Trace elements are indispensable nutrients for animals. Adding certain high-quality and high-utility trace elements in the diet is beneficial to animals. Health and maximizing their productive potential are of paramount importance (NRC, 1998; Richards, 2008). [0003] Manganese (Mn) is a component of several enzyme systems including manganese-specific glycosyltransferase and phosphoenolpyruvate carboxykinase and is required for normal bone structure. In addition, manganese plays an important role in embryonic and postnatal skeletal development. Like copper and zinc, manganese is also involved in the antioxidant effect of cells, and manganese is also a cofactor of superoxide dismutase (Elinor M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A23K1/16C12R1/07C12R1/10C12R1/125
Inventor 潘军张伟强王瑞平苏全马春平
Owner GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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