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A method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum

A technology for purifying polyphenols and compounds, applied in the directions of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve the problems of unfavorable development and utilization of polyphenolic compounds of polyphenols, degradation of polyphenolic compounds, and many impurities of polyphenols, etc. The effect of improving in vitro antioxidant activity, improving extraction rate and reducing degradation

Active Publication Date: 2021-11-16
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flash extraction method has been applied to the extraction of various chemical substances at present, but because the flash extraction relies on the high-speed rotation of the rotor to grind the drug, this process will generate heat, so even the flash extraction method is possible Can cause the degradation of polyphenolic compound; Moreover, the impurity of the polyphenolic substance extracted by simply adopting the flash extraction method is more, has influenced its in vitro antioxidant activity, is unfavorable for the further development and utilization of Polygonatum polyphenolic compound

Method used

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  • A method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum

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Effect test

Embodiment 1

[0041] Embodiment 1: Extraction and purification of polygonatum polyphenols

[0042] (1) Sample pretreatment: pulverize Rhizoma Polygonatum into 40-mesh granules, soak for 15 minutes in an aqueous solution of sodium citrate with a mass concentration of 2%, and dry to obtain a pretreated Rhizoma Rhizoma Polygonatum sample;

[0043] (2) flash type extraction: in the sealwort sample after pretreatment, add the ethanol solution of 30 weight times and carry out flash type extraction, the volume concentration of described ethanol solution is 65%, and extraction voltage is 120V, and extraction time is 50s, and extraction number of times 2 times, collecting the extract, centrifuging the extract, separating and removing solid impurities, and then rotary evaporating to remove the ethanol in the extract to obtain the crude extract of Polygonatum Polyphenols;

[0044] (3) Elution and purification: Use AB-8 macroporous resin to elute and purify the crude Polygonatum polyphenols extract, di...

Embodiment 2

[0045] Embodiment 2: Extraction and purification of polygonatum polyphenols

[0046] (1) Sample pretreatment: pulverize Rhizoma Polygonatum into 40-mesh granules, soak in an aqueous solution of 1% sodium citrate for 15 minutes, and dry to obtain a pretreated Rhizoma Rhizoma Polygonatum sample;

[0047] (2) flash type extraction: in the sealwort sample after pretreatment, add the ethanol solution of 25 weight times and carry out flash type extraction, the volume concentration of described ethanol solution is 60%, and extraction voltage is 120V, and extraction time is 55s, and extraction number of times 2 times, collecting the extract, centrifuging the extract, separating and removing solid impurities, and then rotary evaporating to remove the ethanol in the extract to obtain the crude extract of Polygonatum Polyphenols;

[0048] (3) Elution and purification: use AB-8 macroporous resin to elute and purify the crude Polygonatum polyphenols extract, dilute the crude Polygonatum po...

Embodiment 3

[0049] Embodiment 3: Extraction and purification of polygonatum polyphenols

[0050] (1) Sample pretreatment: Pulverize Polygonatum into 40-mesh granules, soak in an aqueous solution of 3% sodium citrate for 10 minutes, and dry to obtain a pretreated Polygonatum sample;

[0051] (2) flash type extraction: in the sealwort sample after pretreatment, add the ethanol solution of 25 weight times and carry out flash type extraction, the volume concentration of described ethanol solution is 70%, and extraction voltage is 120V, and extraction time is 50s, and extraction number of times 2 times, collecting the extract, centrifuging the extract, separating and removing solid impurities, and then rotary evaporating to remove the ethanol in the extract to obtain the crude extract of Polygonatum Polyphenols;

[0052] (3) Elution and purification: use AB-8 macroporous resin to elute and purify the Polygonatum polyphenols crude extract, dilute the Polygonatum polyphenols crude extract to 0.9...

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Abstract

The invention discloses a method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum, which comprises the following steps: crush Rhizoma Polygonatum into 40-mesh granules, soak in an aqueous solution containing sodium citrate for 10-15 minutes, and dry; add 25-30 The ethanol solution with twice the weight is subjected to flash extraction to obtain the crude Polygonatum polyphenols extract; the crude Polygonatum polyphenols is eluted and purified by using a macroporous resin to obtain purified Polygonatum polyphenols. The present invention effectively reduces the degradation of polyphenols in Polygonatum polyphenols in the flash extraction process through the pretreatment of Polygonatum, improves the extraction rate of Polygonatum polyphenols; Carried out elution and purification, the purity of Polygonatum polyphenols was increased to more than 94.2%, which laid the foundation for the acquisition of polyphenolic compound monomers. At the same time, it also significantly improved the in vitro antioxidant activity of Polygonatum polyphenols extract, which is beneficial to Polygonatum Polyphenols Compounds Further Widely Utilized.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum. Background technique [0002] Polygonatum sibiricum (Polygonatum sibiricum) is a herbaceous plant in the family Liliaceae, and is divided into Polygonatum sibiricum, Polygonatum sibiricum, Polygonatum sibiricum, and Polygonatum sibiricum. As a traditional Chinese medicine in my country, it has been discovered and used as a medicine as early as 2,000 years ago because of its functions of nourishing qi, tonifying the kidney, nourishing the spleen, nourishing yin, and moistening the lungs. It is mostly used to treat coughs, lung diseases and Regulate immunity. A large number of studies have shown that Polygonatum has various physiological functions, such as anti-pathogenic microorganisms, anti-virus, anti-fatigue, lowering blood sugar, lowering blood fat, delaying ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8969A61P39/06
CPCA61K36/8969A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P39/06
Inventor 巫永华刘恩岐张建萍陈尚龙陈安徽邵颖石亿元何智敏
Owner XUZHOU UNIV OF TECH
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