Preparation method and application of cationic peptides of sesame pomace

A technology of cationic polypeptide and sesame meal, which is applied in the preparation of anti-aging functional food by cationic polypeptide of sesame meal, in the field of preparation of cationic polypeptide of sesame meal, can solve the problem of low added value in agriculture, and achieve the purpose of prolonging healthy life and good health. Antioxidant activity in vitro, effect of great economic value

Inactive Publication Date: 2016-05-11
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

About 50% of sesame is used for oil extraction every year, and the by-product—sesame meal is about 150,000 tons. The latter is mainly used as agricultural fertilizer or feed, and the added value of agriculture is relatively low

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  • Preparation method and application of cationic peptides of sesame pomace
  • Preparation method and application of cationic peptides of sesame pomace
  • Preparation method and application of cationic peptides of sesame pomace

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of sesame meal cationic polypeptide

[0017] After crushing the dried sesame meal, collect the powder; weigh 100g of the powder, add 1000mL of 20mM ammonium acetate-acetic acid buffer at pH 5, extract at room temperature for 8h, centrifuge at 8000rpm for 30min to remove the precipitate, and collect the supernatant; Filter through gauze to remove the bran, slowly and evenly add solid ammonium sulfate to 80% saturation, stir at room temperature for 4 hours; centrifuge at 8000rpm for 30min, collect the precipitate; redissolve the precipitate with 30mL of ammonium acetate-acetic acid buffer (pH5.0), 12000rpm Centrifuge for 30min and load the sample on HiPrep TM 26 / 10 desalting column, remove ammonium sulfate, collect protein peaks to obtain crude extract of sesame meal cationic polypeptide; load the crude extract on HiTrap TM The DEAEFF weak anion exchange column was used for separation and purification, the breakthrough peak was collected, an...

Embodiment 2

[0018] Example 2: OH free radical scavenging ability of sesame meal cationic polypeptide

[0019] The sesame meal cationic polypeptide prepared in Example 1 was prepared in different concentration gradients, and the experiment was carried out according to the following method. Add 2.0mL of 6mmol / L ferrous sulfate solution, 2.0mL of cationic polypeptide solution of sesame meal with different mass concentrations, and 2.0mL of 6mmol / L hydrogen peroxide solution in a 10mL test tube, shake well and let it stand for 10min, then add 6mmol / L salicylic acid Acid solution 2.0mL, shake well, let it stand for 30min, then measure its absorbance at a wavelength of 510nm. Repeat the measurement 3 times.

[0020] Clearance rate / %=(1-(A S -A X ) / A 0 )×100

[0021] In the formula: A 0 is the background absorbance without adding sample solution; A S is the absorbance after adding the sample solution to react; A X is the absorbance of the enzymatic hydrolyzate without adding salicylic aci...

Embodiment 3

[0022] Example 3: Effects of Sesame Meal Cationic Polypeptides on the Lifespan of Caenorhabditis elegans

[0023] The effect of cationic peptides from sesame meal on the lifespan of model organism Caenorhabditis elegans was determined by the method of pentafluorouracil (FUDR). The specific operation is as follows: the concentration of the polypeptide drug group is 12.5ug / mL, and a control group is set up. Each group of medium contained 75mM FUDR to inhibit the incubation of nematode progeny. The synchronized L4 stage nematodes were picked into the medium of each group, each group containing 60 nematodes, and cultured at 20°C. The survival days of nematodes were calculated from the moment of transfer, and the day of transfer was recorded as the 4th day of the lifespan experiment. Check the nematodes every other day, record the number of surviving nematodes, and exclude escape, accidental death and bag-like worms. The experimental results show that: 12.5 μg / mL cationic polype...

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Abstract

The invention provides a preparation method of cationic peptides of sesame pomace (CPSP) and an application of the cationic peptides in the preparation of functional foods. The preparation method of the CPSP comprises the steps of smashing sesame pomace, adding a protein extracting solution for extracting, adding ammonium sulfate precipitation peptides, and performing desalting, anion exchange chromatography and the like to obtain the pure CPSP. The CPSP have good antioxidant activity, can significantly prolong the healthy life of caenorhabditis elegans, and can be applied to the anti-aging functional foods serving as functional factors.

Description

technical field [0001] The present invention relates to the preparation and application of plant-derived functional polypeptides, in particular to a method for preparing sesame meal cationic polypeptides and the application of the prepared sesame meal cationic polypeptides in the preparation of anti-aging functional foods. technical background [0002] Sesame is a plant of the genus Sesamum.indicum.L in the family Pedecliecease, and is one of the four major oil crops in my country. In ancient China, sesame has always been regarded as a food for prolonging life. "Baopuzi" records that sesame is "resistant to rheumatism and nourishes aging". About 50% of sesame is used for oil extraction every year, and about 150,000 tons of sesame meal is produced as a by-product. The latter is mainly used as agricultural fertilizer or feed, and the added value of agriculture is relatively low. In recent years, medical and food nutrition studies have shown that sesame meal contains many bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/36C07K1/30C07K1/18A23L33/185
CPCC07K14/415
Inventor 崔晓东王转花马晓丽
Owner SHANXI UNIV
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