Method for improving biological activity of sargassum fusiforme polysaccharide

A hijiki polysaccharide and biologically active technology, applied in the field of chemical engineering, to achieve the effect of lowering cholesterol content, less impact, and mild action conditions

Active Publication Date: 2019-04-16
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing methods for the degradation of hijiki polysaccharides are mostly oxidative degradation and acid degradation methods, and the enzymatic degradation of hijiki polysaccharides is rarely reported.

Method used

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  • Method for improving biological activity of sargassum fusiforme polysaccharide
  • Method for improving biological activity of sargassum fusiforme polysaccharide
  • Method for improving biological activity of sargassum fusiforme polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0043]Weigh 1g of Sargassum polysaccharide (SFP) and dissolve it in 100mL citric acid-sodium citrate buffer (pH 6.0), add pectinase (30,000 U / g, 437.4 mg) and glucoamylase (100,000 U / g mL, 39.8μL) (activity ratio is 3.3:1), and then diluted to 250mL with the same acetic acid-sodium acetate buffer solution, so that the final concentration of polysaccharide in the resulting system is 4mg / ml, and the final concentration of enzyme is 68.4U / mL. The system was placed in a water bath at 54.2° C., oscillating (frequency 220 r / min) for 3 hours. Then heat in a water bath above 90°C for 15 minutes to inactivate the enzyme, filter, and concentrate the clear liquid (filtrate) by rotary steaming (60-70°C) to 1 / 4 of the original volume, dialyze for 48 hours with a dialysis bag with a molecular weight cut-off of 3500Da, and dialyze the The solution (the solution left in the dialysis bag) was concentrated by rotary evaporation (concentrated at 60-70°C to 25% of the volume of the dialysate) and...

Embodiment 1-2

[0050] The shaking reaction time was changed to 4 hours, and the rest was the same as that in Example 1-1 to obtain 882 mg of hijiki enzymatic polysaccharide (ESFP1-2), and the degradation rate of the polysaccharide obtained under this condition was 18.7%.

Embodiment 1-3

[0052] The shaking reaction time was changed to 1h, the activity ratio of pectinase and glucoamylase was 3:1, and the rest was the same as in Example 1-1 to obtain 913 mg of hijiki enzymatic polysaccharide (ESFP1-3). The polysaccharide obtained under this condition The degradation rate is 13.8%.

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Abstract

The invention discloses a method for improving the biological activity of sargassum fusiforme polysaccharide. The method comprises the following steps that crude sargassum fusiforme polysaccharide issubjected to a degradation reaction in a buffer solution with a pH value of 4.5-7.0 under the action of a complex enzyme composed of pectinase and glucoamylase; an obtained reaction liquid is filteredafter enzymes are removed, an obtained filter liquor is concentrated and then dialyzed by a dialysis bag with a molecular cut off of 3500 Da, an obtained dialyzed liquid is concentrated, frozen and dried to obtain sargassum fusiforme complex enzymolysis polysaccharide ESFP. The method not only can obviously improve the antioxidant activity and ability to be combined with cholic acid of the sargassum fusiforme polysaccharide, but also improve the immunomodulatory activity of the sargassum fusiforme polysaccharide to macrophages.

Description

technical field [0001] The invention belongs to the technical field of chemistry and chemical engineering, and relates to a method for enzymolyzing hijiki polysaccharides to enhance their biological activity. Background technique [0002] Hijiki contains various bioactive components with health benefits, especially the acidic polysaccharides, which have antiviral, antifungal, antibacterial, antioxidant, anti-inflammatory, hypolipidemic, and antitumor properties. Because hijiki is cheap and widely distributed, processing hijiki into functional food or developing drugs with low side effects has a good research prospect. The content of carbohydrates in hijiki is relatively high, but the biological activity of crude polysaccharides is relatively low. The biological activity of polysaccharides is closely related to its molecular structure, including glycosidic bonds, monosaccharide composition, sulfate content and molecular weight, etc. High molecular weight polysaccharides are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/04C12P19/14
Inventor 周涛杨斯淇
Owner ZHEJIANG GONGSHANG UNIVERSITY
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