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Method for extracting purified polyphenol compound from rhizoma polygonati

A technology for purifying polyphenols and compounds, applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve the problems of unfavorable development and utilization of polyphenolic compounds of polyphenols, degradation of polyphenolic compounds, and many impurities of polyphenols, etc. The effect of improving in vitro antioxidant activity, improving extraction rate and reducing degradation

Active Publication Date: 2018-11-13
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flash extraction method has been applied to the extraction of various chemical substances at present, but because the flash extraction relies on the high-speed rotation of the rotor to grind the drug, this process will generate heat, so even the flash extraction method is possible Can cause the degradation of polyphenolic compound; Moreover, the impurity of the polyphenolic substance extracted by simply adopting the flash extraction method is more, has influenced its in vitro antioxidant activity, is unfavorable for the further development and utilization of Polygonatum polyphenolic compound

Method used

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  • Method for extracting purified polyphenol compound from rhizoma polygonati

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Extraction and purification of Polygonatum polyphenols

[0042] (1) Sample pretreatment: pulverize Polygonatum into 40 mesh particles, soak it in an aqueous solution of sodium citrate with a mass concentration of 2% for 15 minutes, and dry to obtain a pretreated Polygonatum sample;

[0043] (2) Flash extraction: Add 30 weight times ethanol solution to the pretreated Polygonatum sample for flash extraction, the volume concentration of the ethanol solution is 65%, the extraction voltage is 120V, the extraction time is 50s, and the number of extractions For 2 times, collect the extract, centrifuge the extract to separate and remove solid impurities, and then rotate to evaporate to remove the ethanol in the extract to obtain a crude polyphenol extract of Polygonatum;

[0044] (3) Elution and purification: Use AB-8 macroporous resin to eluate and purify the crude polyphenol extract of Polygonatum. Dilute the crude extract of Polygonatum polyphenol to 0.8mg / ml and load th...

Embodiment 2

[0045] Example 2: Extraction and purification of Polygonatum polyphenols

[0046] (1) Sample pretreatment: pulverize Polygonatum into 40 mesh particles, soak it in an aqueous solution of sodium citrate with a mass concentration of 1% for 15 minutes, and dry to obtain a pretreated Polygonatum sample;

[0047] (2) Flash extraction: Add 25 weight times ethanol solution to the pretreated Polygonatum sample for flash extraction, the volume concentration of the ethanol solution is 60%, the extraction voltage is 120V, the extraction time is 55s, and the number of extractions For 2 times, collect the extract, centrifuge the extract to separate and remove solid impurities, and then rotate to evaporate to remove the ethanol in the extract to obtain a crude polyphenol extract of Polygonatum;

[0048] (3) Elution and purification: Use AB-8 macroporous resin to eluate and purify the crude polyphenol extract of Polygonatum. Dilute the crude extract of Polygonatum polyphenol to 0.7mg / ml and load th...

Embodiment 3

[0049] Example 3: Extraction and purification of Polygonatum polyphenols

[0050] (1) Sample pretreatment: pulverize Polygonatum into 40 mesh particles, soak it in an aqueous solution of sodium citrate with a mass concentration of 3% for 10 minutes, and dry to obtain a pretreated Polygonatum sample;

[0051] (2) Flash extraction: Add 25 weight times ethanol solution to the pretreated Polygonatum sample for flash extraction, the volume concentration of the ethanol solution is 70%, the extraction voltage is 120V, the extraction time is 50s, and the number of extractions For 2 times, collect the extract, centrifuge the extract to separate and remove solid impurities, and then rotate to evaporate to remove the ethanol in the extract to obtain a crude polyphenol extract of Polygonatum;

[0052] (3) Elution and purification: The AB-8 macroporous resin was used to eluate and purify the crude polyphenol extract of Polygonatum. Dilute the crude extract of Polygonatum polyphenol to 0.9mg / ml an...

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Abstract

The invention discloses a method for extracting a purified polyphenol compound from rhizoma polygonati. The method comprises the following steps: crushing the rhizoma polygonati into grains with the size of 40 meshes and putting the rhizoma polygonati grains into a water solution containing sodium nitrate; immersing for 10 to 15min and drying; adding an ethanol solution which is 25 to 30 times ofthe weight of and carrying out flash type extraction to obtain a rhizoma polygonati polyphenol crude extraction solution; carrying out elution and purification on the rhizoma polygonati polyphenol crude extraction solution by adopting macroporous resin to obtain purified rhizoma polygonati polyphenol. According to the method disclosed by the invention, the rhizoma polygonati is pre-treated, the degradation of the polyphenol compound in the rhizoma polygonati in a flash extraction process is effectively reduced and the extraction rate of the rhizoma polygonati polyphenol is improved; the rhizoma polygonati polyphenol crude extraction solution is eluted and purified by adopting the macroporous resin and the purity of the rhizoma polygonati polyphenol is improved to 94.2 percent or more; a foundation is laid for obtaining a polyphenol compound monomer; meanwhile, the in-vitro antioxidant activity of rhizoma polygonati polyphenol extract is also remarkably improved; a rhizoma polygonati polyphenol compound is further widely utilized.

Description

Technical field [0001] The invention relates to the technical field of extraction of plant active ingredients, in particular to a method for extracting and purifying polyphenol compounds from Polygonatum. Background technique [0002] Polygonatum sibiricum (Polygonatum sibiricum) is a herbaceous plant in the Liliaceae family, which is divided into Polygonatum sibiricum, Polygonatum sibiricum, Polygonatum sibiricum and Polygonatum sibiricum. As a traditional Chinese medicine in my country, it has been found and used as a medicine more than 2000 years ago because of its qi, kidney, spleen, yin, lung and other effects. It is mostly used to treat cough, lung diseases and Regulate immunity. A large number of studies have shown that Polygonatum has a variety of physiological functions, such as anti-pathogenic microorganisms, anti-virus, anti-fatigue, lowering blood sugar, lowering blood lipids, delaying aging, regulating immunity, improving memory and better antioxidant capacity. Mode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61P39/06
CPCA61K36/8969A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A61P39/06
Inventor 巫永华刘恩岐张建萍陈尚龙陈安徽邵颖石亿元何智敏
Owner XUZHOU UNIV OF TECH
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