Extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing extraction method

An extraction method and a technology for total flavonoids are applied in the field of extraction methods for total flavonoids from Pterocarpus and the field of extraction equipment applying the method, which can solve the problems of difficult removal of filter paper, poor sealing performance, poor filtering effect, etc., and achieve low extraction cost and increase in extraction cost. Reduced activity, significant antioxidant effect

Active Publication Date: 2019-07-12
LANZHOU UNIVERSITY
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Problems solved by technology

[0004] The purpose of the present invention is to explore the optimal extraction process of total flavonoids of Zeraria, and carry out preliminary research on the components of total flavonoids of Bracken and its antioxidant effect, and propose a method for extracting total flavonoids of Bracken and its antioxidant effect Determination has effectively improved the extraction rate of the total flavonoids of Bracken, and confirmed that the extract of Bracken has antioxidant properties. In order to solve the problems of poor effect and difficult removal of the filter paper after filtration, a decompression filtration device for extracting total flavonoids of Bracken was also proposed, which effectively improved the sealing of the device, and the filtration effect was better and the filter paper was easy to remove after the filtration work was completed. Down

Method used

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  • Extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing extraction method
  • Extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing extraction method
  • Extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] see Figure 1-5 , a method for extracting total flavonoids from Bracken, comprising the following steps;

[0056] A method for extracting total flavonoids from Bracken, comprising the following steps;

[0057] S1. Take an appropriate amount of junima tubers, wash the junima tubers and put them in a drying oven at 30°C to dry to constant weight, then crush the dried junima tubers into juna tuber powder, and put them in a desiccator for later use ;

[0058] S2. Weigh a certain amount of fern root powder and move it into a round bottom flask, then add different concentrations of ethanol solutions, and prepare mixed solutions with different material-liquid ratios according to the needs of the experiment, then put the round bottom flask into the microwave oven, set a certain The microwave power, the microwave temperature and the microwave time are obtained to obtain the extract;

[0059] S3, take a part of the extract for processing, and then use the extract to measure it...

Embodiment 2

[0085] Based on Example 1 but with some differences;

[0086] Extraction of Total Flavonoids from Bracken

[0087] After cleaning the fern tuber, put it into a drying oven at 30°C and dry to constant weight, and put it into a desiccator after crushing for subsequent use. Accurately weigh 5g of Juema root tuber powder and transfer it into a round bottom flask, add ethanol solutions of different concentrations, extract at a certain microwave power and temperature for different times, filter the extract under reduced pressure to remove the filter residue, transfer to a 100mL volumetric flask and use 60 % ethanol to the mark, shake well and set aside.

[0088] One-way optimal level analysis

[0089] Study ethanol concentration (0, 25, 50, 75, 100%, v / v), solid-liquid ratio (1:10, 1:15, 1:20, 1:25, 1:30), microwave radiation time (1 , 2, 3, 4, 5min), microwave radiation temperature (40, 50, 60, 70, 80, 90°C) four factors on the extraction of flavonoids from the root of Bracken. ...

Embodiment 3

[0098] Based on Example 1 and 2 but different:

[0099] Determination of antioxidant capacity by DPPH method

[0100] Dissolve the fern extract in 100 μl of 50% methanol according to different masses, and mix it with 900 μl of DPPH free radical solution, so that the concentrations are 3.125, 6.25, 12.5, 25, and 50 μg / mL, and use the same volume of 50% methanol as a blank control. After standing in the dark for 30min, the absorbance was measured at 517nm by a spectrophotometer. The scavenging capacity of DPPH was calculated by the following formula

[0101] Clearance rate (%)=(1-(A drug / A control))×100

[0102] Determination of antioxidant capacity by ABTS+ method

[0103] 2.0mmol / LABTS was added to 2.45mmol / L potassium persulfate solution and treated for 4 hours at room temperature in the dark to generate ABTS+ free radicals. Dilute ABTS+ with 0.1M sodium phosphate buffer (pH7.4), and measure the absorbance at 734nm. Dissolve the fern extract in 50 μl 50% methanol accord...

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Abstract

The invention discloses an extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing the extraction method, and belongs to the technical field of total flavone extraction. According to the extraction method, the extraction rate is taken as a main inspection index, factors of an extraction agent, a solid-liquid ratio, extraction time, the extraction temperature and the like are considered, an extraction method for the total flavone of the potentilla anserine L. through microwave-assisted extraction is optimized, and the anti-oxygenation effect and components of the flavone of the potentilla anserine L. are studied primarily; the technology process is simple, the requirements for the production equipment and technology are low, the extraction cost is low, and the extraction efficiency of the total flavone is high; higher popularization and application value is achieved, and the flavone of the potentilla anserine L. has good in-vitro antioxidant activity and is a novel natural antioxidant or a functional food.

Description

technical field [0001] The invention relates to the technical field of extracting total flavonoids, in particular to a method for extracting total flavonoids from Bracken and extraction equipment using the method. Background technique [0002] Potentiiia anserine L. is the tuber of Potentiiia anserine L., a plant of the genus Potentii in the family Rosaceae. It was first recorded in "Commonly Used Chinese Medicinal Materials in Tibet". It is a commonly used Tibetan medicine with a history of more than 1,200 years. It can not only be used as Daily consumption of food can also be used as medicine to cure diseases and strengthen the body. It has the effects of invigorating the spleen and stomach, promoting body fluid and relieving cough, nourishing qi and blood, nourishing yin and nourishing the kidney. Kidney deficiency and Qi deficiency, anemia after illness, malnutrition and other diseases. Bracken is rich in carbohydrates, proteins, fatty acids, triterpenoids, flavonoids, ...

Claims

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Application Information

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IPC IPC(8): B01D11/02B01D29/085B01D29/58B01D29/96A61K36/73A61P39/06
CPCA61K36/73A61K2236/333A61P39/06B01D11/02B01D11/0288B01D29/085B01D29/58B01D29/96B01D2201/204B01D2201/34
Inventor 程菊刘迪赵丽霞沈蓉白德成
Owner LANZHOU UNIVERSITY
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