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Method for extracting and purifying polysaccharides from orange peels

A technology for citrus peel and polysaccharide, applied in the field of polysaccharide extraction, can solve the problems of low product purity, no public extraction and purification of polysaccharide, etc., and achieve the effects of high product purity, huge application value and low energy consumption

Inactive Publication Date: 2017-05-31
佛山市海科知识产权交易有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage is that the active extract of citrus peel of this invention contains a large amount of protein, pigment and other small molecular impurities, and the product purity is low, and the active extract of citrus peel of this invention is mainly a mixture of citrus flavonoids and pectin polysaccharides, and has no Disclosed a technical solution for the separate extraction and purification of polysaccharides from citrus peels

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] 1) Preparation of crude citrus peel polysaccharide

[0024] Crush the citrus peel, weigh the citrus peel powder in a distillation flask, add distilled water, let it stand at room temperature, process it in a microwave device for 2-3 hours, then place it in a constant temperature water bath, heat and reflux for extraction, and extract the filter residue repeatedly. Combine, concentrate with a rotary evaporator, add absolute ethanol to seal, let stand, and filter the polysaccharide crude product;

[0025] 2) Purification of crude polysaccharides from citrus peel

[0026] A. Activated carbon decolorization

[0027] Put the crude polysaccharide in a beaker, dissolve it with distilled water, add activated carbon, stir in a water bath for 30-45 minutes, filter, and repeat the decolorization to obtain the crude polysaccharide solution after decolorization;

[0028] B. Deproteinization by Sevag method

[0029] Take the polysaccharide aqueous solution in A, add chloroform sol...

Embodiment 1

[0037] 1) Preparation of crude citrus peel polysaccharide

[0038] Crush the citrus peel, weigh 100g of citrus peel powder in a distillation flask, add 100ml of distilled water, let it stand at room temperature for 12 hours, process it in a microwave device with a power setting of 900W for 3 hours, and then place it in a constant temperature water bath with a temperature setting of 78°C Extract by heating and reflux for 2 hours, extract the filter residue twice, the extraction time is 3 hours and 1 hour respectively, combine the extracts, concentrate with a rotary evaporator to 1 / 3 of the original volume, add absolute ethanol which is 3 times the volume of the concentrated solution, and seal , stand still for 12h, and the polysaccharide crude product after suction filtration;

[0039] 2) Purification of crude polysaccharides from citrus peel

[0040] A. Activated carbon decolorization

[0041] Weigh 20 g of the crude polysaccharide and place it in a beaker, dissolve it with ...

Embodiment 2

[0046] 1) Preparation of crude citrus peel polysaccharide

[0047] Crush the citrus peel, weigh 100g of citrus peel powder into a distillation flask, add 150ml of distilled water, let it stand at room temperature for 12 hours, process it in a microwave device with a power setting of 800W for 2 hours, and then place it in a constant temperature water bath with a temperature setting of 80°C within 1 hour of heating and reflux for extraction, the filter residue was extracted twice, the extraction time was 4 hours and 1 hour respectively, the extracts were combined, concentrated to 1 / 4 of the original volume with a rotary evaporator, and 4 times the volume of the concentrated solution was added with absolute ethanol to seal , stand still for 12h, and the polysaccharide crude product after suction filtration;

[0048] 2) Purification of crude polysaccharides from citrus peel

[0049] A. Activated carbon decolorization

[0050] Weigh 20 g of the crude polysaccharide and place it i...

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Abstract

The invention discloses a method for extracting and purifying polysaccharides from orange peels. The method includes the steps of firstly, preparing crude orange peel polysaccharides; secondly, purifying the crude orange peel polysaccharides, to be more specific, performing activated carbon decoloration, and using a Sevag method to perform deproteinization. The method has the advantages that the high-purity polysaccharides are obtained from the orange peels through the simple and efficient experiment method, the prepared polysaccharides have a certain anti-oxidation activity and are high in purity and low in energy consumption, and the high-purity polysaccharides have a huge application value in fields such as medicine, healthcare products and food.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for extracting and purifying polysaccharides from citrus peels. Background technique [0002] Citrus peel, also known as tangerine peel, is the dry and mature peel of the rue plant tangerine and its cultivars. It is bitter, pungent, warm in nature, and has the functions of regulating qi, invigorating the spleen, drying dampness and reducing phlegm. According to data, pectin polysaccharides in citrus peel The content can reach 10% to 30%. Polysaccharide refers to a high molecular polymer with more than 10 monosaccharide molecules connected by glycosidic bonds, which may include hundreds or even thousands of monosaccharide molecules. In addition to monosaccharides, the polysaccharide structure sometimes contains uronic acid, amino sugar and Sugar alcohols, etc. Polysaccharides widely exist in animals, plants, microorganisms, and marine organisms in nature. They a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王滔
Owner 佛山市海科知识产权交易有限公司
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