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Method for extracting hovenia dulcis thunb total flavones by cooperation of surfactant and microwave-ultrasonic extraction process

A technology of surfactant and Hovenia dulcis, which is applied in the field of extraction of total flavonoids from Hovenia dulcis by using surfactants in conjunction with microwave-ultrasonic waves, can solve toxicity and other problems, achieve low cost, avoid thermal effects, and consume less energy

Inactive Publication Date: 2014-04-30
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current synthetic antioxidants BHT, BHA, and TBHA have certain toxicity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting total flavonoids from Hovenia dulcis fruit with surfactants combined with microwave-ultrasonic waves, comprising the following steps: (1) Accurately weigh 5 g of Hovenia dulcis fruit pulverized, vacuum-dry, and perform Soxhlet extraction with petroleum ether until the fat is completely removed to obtain 4.59g of completely degreased pulverized Hovenia dulcis seeds; (2) Mix 4.59g of completely degreased Hovenia dulcis seeds, 50g of distilled water and 0.4g of surfactant Tween 20 to obtain a mixed solution; (3) mix The mixed solution is subjected to microwave-ultrasonic extraction, the microwave power is 300W, the ultrasonic power is 400W, and the extraction time is 10min; (4) after the extraction is completed, filter the mixed solution and take the filtrate; (5) add 60vt% (volume Concentration) ethanol for alcohol precipitation, after standing still, the alcohol supernatant was discarded, and then concentrated and dried to obtain the total flavonoi...

Embodiment 2

[0027] A method for extracting total flavonoids from Hovenia dulcis fruit with surfactants combined with microwave-ultrasonic waves, comprising the following steps: (1) Accurately weigh 5 g of Hovenia dulcis fruit pulverized, vacuum-dry, and perform Soxhlet extraction with petroleum ether until the fat is completely removed to obtain 4.59g of completely degreased pulverized Hovenia dulcis seeds; (2) 4.59g of completely degreased pulverized Hovenia dulcis seeds, 100g of distilled water and 0.2g of surfactant Tween 40 were mixed uniformly to obtain a mixed solution; (3) The mixed solution is subjected to microwave-ultrasonic extraction, the microwave power is 200W, the ultrasonic power is 100W, and the extraction time is 5min; (4) after the extraction is completed, filter the mixed solution and take the filtrate; (5) add 80vt% ethanol to the filtrate for Alcohol precipitation, discard the alcohol supernatant after standing, and then concentrate and dry to obtain the total flavono...

Embodiment 3

[0031] A method for extracting total flavonoids from Hovenia dulcis Fructus in conjunction with microwave-ultrasonic waves, comprising the steps of:

[0032] (1) Accurately weigh 5g of the crushed Hovenia dulcis fruit, vacuum-dry it, and perform Soxhlet extraction with petroleum ether until completely degreased to obtain 4.59g of completely degreased Hovenia dulcis; (2) Completely degrease Hovenia dulcis 4.59g of crushed matter, 150g of distilled water and 3g of surfactant Tween 60 were mixed uniformly to obtain a mixed solution; (3) the mixed solution was subjected to microwave-ultrasonic extraction, the microwave power was 600W, the ultrasonic power was 500W, and the extraction time was 20min; (4) After the extraction, filter the mixed solution and take the filtrate; (5) Add 40vt% ethanol to the filtrate for alcohol precipitation, discard the alcohol supernatant after standing, and then concentrate and dry to obtain Hovenia dulcis. flavonoids.

[0033] Using NaNO 2 -Al(NO ...

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PUM

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Abstract

The invention particularly relates to a method for extracting hovenia dulcis thunb total flavones by cooperation of a surfactant and a microwave-ultrasonic extraction process. The method comprises the following steps: (1) grinding hovenia dulcis thunb, and performing Soxhlet extraction by petroleum ether to remove fat so as to obtain fully defatted ground hovenia dulcis thunb; (2) uniformly mixing the ground hovenia dulcis thunb, distilled water and the surfactant to obtain a mixed solution; (3) performing microwave-ultrasonic extraction on the mixed solution at the microwave power of 200-600 W and the ultrasonic power of 100-500 W for 5-20 minutes; (4) after the extraction, performing alcohol precipitation on the extracted filtrate to remove alcohol supernatant, and concentrating and drying to obtain a hovenia dulcis thunb total flavones extract. Due to the adoption of the method for extracting hovenia dulcis thunb total flavones, the extraction time is greatly shortened, the extraction efficiency is improved, the cost is reduced, and the energy consumption is low; meanwhile, a distilled water solution in which a small amount of the surfactant is dissolved is taken as an extraction solution, so that the problem of toxic organic solvent residues is solved.

Description

technical field [0001] The invention relates to a method for extracting natural active substances, in particular to a method for extracting total flavonoids from Hovenia dulcis fruit by using a surfactant in cooperation with microwave-ultrasonic waves. Background technique [0002] Hovenia dulcis is a plant of Rhamnaceae Hovenia trifoliate ( Hoveniadulcis Thunb ) of dried seeds. According to traditional Chinese medicine, Hovenia citrus has the functions of quenching thirst and relieving restlessness, clearing away dampness and heat, and detoxifying alcohol. It is used for restorative heat and quenching thirst, constipation, alcoholism and other diseases. Among them, the main active ingredients of Hovenia dulcis are flavonoids (including kaempferol, acetin, myricetin, quercetin, and dihydromyricetin) and anthraquinone compounds such as emodin. At present, the methods for extracting flavonoids from Hovenia dulcis Fructus mainly include traditional solid-liquid extraction me...

Claims

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Application Information

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IPC IPC(8): A61K36/72A61K131/00
Inventor 吕萌黎莉
Owner WUHAN INSTITUTE OF TECHNOLOGY
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