Fructus sophorae total flavonoid extract with broad-spectrum anti-tumor activity and preparation method and application of fructus sophorae total flavonoid extract

Abstract

The invention provides a fructus sophorae total flavonoid extract with broad-spectrum anti-tumor activity and a preparation method and application of the fructus sophorae total flavonoid extract. The fructus sophorae total flavonoid extract at least comprises quercetin, genistein, kaempferol and isorhamnetin according to the mass ratio of (2-3): (14-16): (15-17): (0.5-1), and has a favorable in-vitro anti-oxidative function, broad-spectrum anti-tumor activity, and a remarkable inhibiting effect on human esophagus cancer, liver cancer, breast cancer, human lymphoma and the like. The preparation method comprises the following steps: taking nut parts of fructus sophorae in autumn as the raw materials; then taking ethanol of 80 percent, 60 percent and 40 percent volume fractions as solvents for gradient ultrasonic extraction, macroporous adsorption resin adsorption and elution; collecting the eluant; concentrating and drying. The preparation method remarkably improves the extraction ratio of fructus sophorae total flavonoids, simplifies the extraction steps and reduces impurity interference.

Description

Technical field [0001] The invention relates to a natural plant total flavonoids and a preparation method and application thereof, in particular to a Sophora japonicus total flavonoids extract with broad-spectrum anti-tumor activity, and a preparation method and application thereof. Background technique [0002] Fructus Sophorae (Fructus Sophorae) is a commonly used Chinese medicinal material included in the Pharmacopoeia of the People's Republic of China (hereinafter referred to as the Pharmacopoeia), and is the dried and mature fruit of the legume Sophorajaponica (Sophorajaponica L.). According to the pharmacopoeia, the medicinal Sophora japonica should usually be mature pods harvested in winter. The dried Sophora japonicus pods picked after the winter solstice are cylindrical and sometimes curved. The seeds are constricted into beads. After the foreskin, they are brown or yellowish brown. The seeds are brown-black. The traditional Chinese medicine theory believes that Sophora ...

AI Technical Summary

Problems solved by technology

In recent years, people have done a lot of research on the flavonoids in Huaijiao and their extraction methods. ZL200810068891.9 discloses the method of extracting flavonoids from Huaijiao and extracting kaempferol. Diatomaceous earth filtration, macroporous resin adsorption, ethanol elution to obtain Sophora flavonoids extract, hydrolyze the Sophora flavonoids extract, filter, and extract the filter residue to obtain high-purity kaempferol, which is the active ingredient in Sophora japonicus. Reasonable utilization provides a new way, but the extraction rate of flavonoids is low by hot water extraction, but it is easy to extract water-soluble impurities such as sugars, which increases the difficulty of separation; ZL200380110595.6 discloses A method for preparing isoflavone-containing Sophora japonicus extract, which is to prepare Sophora japonicus extract with high concentration of aglycon-type isoflavones with good bioabsorbability by hot water extraction, enzyme treatment and ethanol extraction, but The use of enzyme treatment not only has high requirements for extraction equipment, but also may change the structure of certain components in Sophora japonica during the extraction process, produce new chemical substances, and affect the purity and yield of the extract; ZL201210323298.0 provides A method for comprehensively extracting genistein and kaempferol from Sophorae japonicus. The mixture of kaempferol and genistein is obtained by hydrolyzing the raw materials with acid water, and then the two are separated by alkaline water extraction. The method is carried out by Extraction of a single component, no study on the extraction of total flavonoids

In recent years, there are few studies on the extraction process of total flavonoids from Sophora japonicus and they stay in the stage of water extraction, and the extraction rate is low. There are also reports using ethanol as a solvent and ultrasonic-assisted extraction, but it is a conventional extraction process, and a single concentration of ethanol is used for extraction. , the application of the extracted total flavonoids of Sophora japonica is rarely reported

[0004] To sum up, the research history of Huaijiao is relatively early, the chemical composition is complex, the pharmacological effects are significant, and the clinical application is extensive. There are few research reports on the extraction and preparation methods of flavonoids, especially the lack of methods that can quickly and effectively extract and prepare the total flavonoids extract of Sophora japonicus with good anticancer activity

Images