Active part of radish leaf and application of active part

A technology of active parts and radish sprouts, which is applied in the field of medicine, can solve problems such as no related reports, and achieve the effect of delaying aging and anti-oxidative aging

Inactive Publication Date: 2017-03-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there are no relevant reports at home and abroad on the

Method used

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  • Active part of radish leaf and application of active part
  • Active part of radish leaf and application of active part
  • Active part of radish leaf and application of active part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of ethyl acetate extraction fraction of radish sprouts 95% ethanol extract:

[0027] Take 100 g of radish powder that has been crushed through a 200-mesh sieve, add 2000 mL of ethanol aqueous solution with a volume fraction of 95%, and extract it in a water bath at 45 ° C. After suction filtration, the filter residue is repeatedly extracted three times, and the filtrates are combined, and the ethanol is recovered by vacuum concentration to obtain radish. Ethanol extract; Ethanol extract of radish sprouts is ultrasonically dispersed in 100mL water, followed by fractional extraction with 50mL petroleum ether, 50mL chloroform, 50mL ethyl acetate, and 50mL water-saturated n-butanol, each extracting 3 times, combining the extracts, and concentrating under reduced pressure The solvent was recovered and freeze-dried to obtain the petroleum ether fraction, the chloroform fraction, the ethyl acetate fraction, the water-saturated n-butanol fraction and the water fracti...

Embodiment 2

[0029] Preparation of Ethyl Acetate Extraction Fraction of 70% Ethanol Extract of Radish Shoots:

[0030] Take 100 g of radish powder that has been crushed through a 200-mesh sieve, add 1000 mL of ethanol aqueous solution with a volume fraction of 70%, and extract it in a water bath at 35°C. After suction filtration, the filter residue is repeatedly extracted three times, and the filtrates are combined, and the ethanol is recovered by vacuum concentration to obtain radish Ethanol extract; Ethanol extract of radish sprouts is ultrasonically dispersed in 100mL water, followed by fractional extraction with 50mL petroleum ether, 50mL chloroform, 50mL ethyl acetate, and 50mL water-saturated n-butanol, each extracting 3 times, combining the extracts, and concentrating under reduced pressure The solvent was recovered and freeze-dried to obtain the petroleum ether fraction, the chloroform fraction, the ethyl acetate fraction, the water-saturated n-butanol fraction and the water fractio...

Embodiment 3

[0032] Effect of ethyl acetate extraction fraction on lifespan delay in Drosophila

[0033] 1. Experimental materials

[0034] Wild-type Drosophila melanogaster was provided by the Genetics Laboratory of Nanjing Agricultural University;

[0035] 2. Dose selection

[0036] Set up a blank control group and two dosage groups, and the concentration of the test substance contained in the culture medium of each dosage group is 0, 5 mg / kg and 15 mg / kg.

[0037] 3. Drosophila Basal Medium

[0038] Corn flour 8.5%, sucrose 6.5%, agar fraction 0.75%, propionic acid 0.5%, yeast powder 0.75%, water 83%, pH=7.

[0039] 4. Drosophila longevity experiment

[0040]Select unmated female fruit flies hatched within 10 hours, anesthetized with ether, and randomly divide them into blank control group, sample high-dose group, sample low-dose group, 20 per tube, 10 tubes per group, at a temperature of 25 ± 1 Cultivate in an incubator with 55-65% humidity and 12 hours of alternating light. Put ...

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Abstract

The invention provides an active part of a radish leaf and application of the active part. The active part is prepared by the following steps: firstly, drying the radish leaf, crushing and enabling the crushed substance to pass through a sieve with 200 meshes; secondly, adding the sieved radish leaf into an aqueous solution of ethanol for leaching at constant temperature; performing suction filtering, repeatedly extracting filter residues for three times, combining filtrate, concentrating under reduced pressure and recovering the ethanol, thus obtaining an ethanol extract of the radish leaf; thirdly, performing ultrasonic dispersion on the ethanol extract of the radish leaf by using water, then performing fractional extraction by sequentially using petroleum ether, chloroform, ethyl acetate and water-saturated normal butanol, respectively extracting for three times, and combining the same extracting solutions; concentrating a combined solvent under reduced pressure, and performing freeze drying to respectively obtain a petroleum ether part, a chloroform part, an ethyl acetate part, a water-saturated normal butanol part and a water part, wherein the ethyl acetate part is the active part of the radish leaf. The prepared active part of the radish leaf has the effect of prolonging the service life of drosophila melanogaster, a good antioxidation effect and potential of delaying senescence.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of ethyl acetate extraction parts with anti-oxidation and anti-aging activities from radish radish and its application in the preparation of anti-oxidation and anti-aging drugs. Background technique [0002] my country is one of the countries with the fastest aging rate in the world and one of the countries with the largest aging population. According to the forecast of WHO, by 2050, my country's elderly population will exceed 400 million, which will bring a heavy burden to the society. Delaying aging is an effective countermeasure to promote healthy aging and an effective measure to improve the quality of life of the elderly population. Delaying aging has become a major topic of global concern. [0003] After the aging organism matures, under normal physiological conditions, with the increase of age, the self-renewal and repair ability of the organi...

Claims

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Application Information

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IPC IPC(8): A61K36/31A61P39/06
CPCA61K36/31A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 罗孝平段玉清
Owner JIANGSU UNIV
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