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Method for improving biological activity of porphyra yezoensis polysaccharide

A technology of Porphyra zebra and biological activity, which is applied in the field of chemistry and chemical engineering, can solve the problems of low biological activity and large molecular weight, and achieve high in vitro anti-oxidation and in vitro immune activity, in vitro immune activity and in vitro bile acid binding ability.

Active Publication Date: 2019-05-24
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The water-extracted polysaccharides of Porphyra zebra are mainly water-soluble sulfated polysaccharides with large molecular weight and relatively low biological activity

Method used

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  • Method for improving biological activity of porphyra yezoensis polysaccharide
  • Method for improving biological activity of porphyra yezoensis polysaccharide
  • Method for improving biological activity of porphyra yezoensis polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0059] S1, dissolving the Porphyra zebra polysaccharide (PSPY) in distilled water to prepare a Porphyra zebra crude polysaccharide solution with a concentration of 10 mg / mL;

[0060] Hydrogen peroxide and ascorbic acid are added to the crude polysaccharide solution of Porphyra zebra, and the mixture is obtained after diluting with distilled water.

[0061] The molar ratio of hydrogen peroxide and ascorbic acid=1:1.

[0062] The final concentrations of hydrogen peroxide and ascorbic acid in the obtained mixed solution were both 4.1 mmol / L, and the final concentration of the crude polysaccharide of Porphyra zebra was 5 mg / mL.

[0063] The obtained mixed solution was incubated and stirred at 52.4°C for 2.1 hours, so as to degrade the crude polysaccharide of Porphyra zebrae and obtain a reaction solution;

[0064] S2. Concentrate the reaction solution obtained in step S1 by rotary evaporation at 60° C. to 1 / 6 of the original volume to obtain a concentrated solution;

[0065] Anh...

Embodiment 2-1

[0081] Accurately weigh 450 mg of the degraded polysaccharide (DPSPY1-1) of P. Ⅰ Evenly add to the upper surface of the chromatography column filler, pass through the DEAE Cellulose-52 chromatography column (the column specification is 60cm×Φ2.6cm, and the effective length is 50cm), and then use deionized water, and the concentration of 0.1mol / L, 0.3mol / L, 0.5mol / L, 0.7mol / L NaCl solutions, five kinds of eluents, were used for elution, and the flow rate was controlled at 1mL / min for 10 minutes per tube, and collected by an automatic collector; the sulfuric acid-phenol method was used to detect the separated tubes The ultraviolet absorption values ​​at 490nm of each group of eluents were obtained, and the elution curves were drawn.

[0082] Note: Use the sulfuric acid-phenol method to detect the ultraviolet absorption value of the eluent at 490nm to determine whether the eluent contains polysaccharides. When it is detected that the eluent corresponding to the eluent does not co...

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Abstract

The invention provides a method for improving biological activity of a porphyra yezoensis polysaccharide. The method comprises the following steps that hydrogen peroxide and ascorbic acid are added into a crude polysaccharide solution and diluted with water to obtain a mixed solution; the obtained mixed solution is subjected to a heat preservation stirring reaction for 1-3 hours at 40-60 DEG C toobtain a reaction liquid; the obtained reaction liquid is concentrated to 15%-20% of the original volume to obtain a concentrated liquid; anhydrous ethanol is added into the concentrated liquid, standing is carried out at 0-5 DEG C overnight, then centrifugation is carried out, and a precipitate is obtained; the obtained precipitate is mixed with deionized water to be dissolved again and dialyzedto obtain a dialyzate I; the obtained dialyzate I is concentrated and then freeze-dried to obtain a porphyra yezoensis degraded polysaccharide. Compared with a porphyra yezoensis crude polysaccharide,the porphyra yezoensis degraded polysaccharide has the advantages that the antioxidant activity in vitro, bile acid binding activity and immunocompetence are all obviously improved. The invention also provides the application of the degraded porphyra yezoensis polysaccharide as a functional food base material prepared by the method.

Description

technical field [0001] The invention belongs to the technical field of chemistry and chemical engineering, and relates to a method for degrading Porphyra zebra polysaccharides by combining hydrogen peroxide and ascorbic acid so as to enhance its biological activity. Background technique [0002] Porphyra variegata is an edible and economical brown seaweed that is widely grown and consumed in East and Southeast Asian countries such as Japan, Korea, and China. Edible sheets of dried variegated laver known as "nori" are traditionally used in Japanese dishes such as sushi. Seaweed is known to contain some biological functional components, such as Porphyra zebra polysaccharides, taurine, polyunsaturated fatty acids, carotenoids and outer membrane porin-like proteins, amino acids and minerals, vitamins and so on. According to the literature reports, Porphyra zebra polysaccharides have physiological functions such as improving immunity of mammals, lowering blood fat, anti-inflamma...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L33/125
Inventor 周涛孔立敏傅玲琳
Owner ZHEJIANG GONGSHANG UNIVERSITY
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