A nitrilase that can hydrolyze terephthalonitrile to p-cyanobenzoic acid

A technology of terephthalonitrile and cyanobenzoic acid, which is applied in the fields of hydrolytic enzymes, genetic engineering, and plant genetic improvement, and can solve problems such as low substrate concentration, low enzyme catalytic efficiency, and long reaction time
CN107641622BActive Publication Date: 2021-10-08TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2021-10-08

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Abstract

The invention discloses nitrilase N1 and its gene derived from Pantoea sp. ) nitrilase N3 and its gene, Leptolyngbya sp. nitrilase N4 and its gene, Brassica oleracea var. oleracea nitrilase N5 and its gene and Camelina sativa nitrilase N6 and its gene, and using the nitrilase as a biocatalyst to prepare p-aminomethylbenzoic acid intermediate p-cyanobenzoic acid. The resting cells of the corresponding nitrilase can catalyze 100g / L of the substrate, and the conversion rate is greater than 99%. The method has the remarkable characteristics of mild reaction conditions, no pollution, simple process route, etc., and has great industrial application prospects.
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Description

technical field

[0001] The present invention relates to genes and protein products thereof, in particular to nitrilase N1 and its gene from Pantoea sp.AS-PWVM4, nitrilase N2 and its gene from Arabidopsis thaliand, agile Nitrilase N3 and its gene from Acidovorax facilis 72W, Nitrilase N4 and its gene from Leptolyngbya sp., Nitrile from Brassica oleracea var. oleracea The hydrolase N5 and its gene, the nitrilase N6 of Camelina sativa and its gene, and its biocatalytic preparation of p-cyanobenzoic acid belong to the field of applied microorganism and enzyme engineering. Background technique

[0002] p-Aminomethylbenzoic acid is a hemostatic agent, suitable for abnormal bleeding during operations on the lung, liver, pancreas, prostate, thyroid, adrenal gland, etc. Hypertrophic bleeding, upper gastrointestinal bleeding, etc. In industry, the catalytic hydrogenation of p-cyanobenzoic acid is mainly used to prepare p-aminomethylbenzoic acid.

[0003] The production method of p-...

Claims

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