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Method for detecting contents of eight flavones and phenolic acid components in camelina sativa seeds

A detection method, the technology of camelina, applied in the field of chromatographic detection, can solve the problems of expensive standard products, less research on seeds, and restrictions on the practical application of multi-index component quality control, and achieve good stability and reproducibility

Pending Publication Date: 2021-07-20
河南省纳普生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The seeds of camelina are mostly used to extract oil, but there are few studies on the degreased seeds. Previous studies have found that the seeds of camelina contain flavonoids and phenolic acids. The content was determined by the method of one test and multiple evaluations. 8 There is no report on the method for the simultaneous determination of flavonoids and phenolic acids, and the practical application of multi-index component quality control is limited due to the high cost of standard products.

Method used

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  • Method for detecting contents of eight flavones and phenolic acid components in camelina sativa seeds
  • Method for detecting contents of eight flavones and phenolic acid components in camelina sativa seeds
  • Method for detecting contents of eight flavones and phenolic acid components in camelina sativa seeds

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Experimental program
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Effect test

Embodiment 1

[0044] A method for detecting the contents of 8 kinds of flavonoids and phenolic acids in camelina seeds, comprising the following steps:

[0045] (1) Preparation of mixed reference substance standard solution

[0046] Eight reference substances of chlorogenic acid, catechin, epicatechin, sinapic acid, rutin, quercetin, isoquercetin, and isorhamnetin were precisely weighed, dissolved and diluted with methanol to obtain a single Reference substance stock solution; wherein, the concentration of chlorogenic acid reference substance stock solution is 92.4 μg / mL, the concentration of catechin reference substance stock solution is 776.16 μg / mL, and the concentration of epicatechin reference substance stock solution is 40.50 μg / mL, the concentration of sinapinic acid reference substance stock solution is 42.40 μg / mL, the concentration of rutin reference substance stock solution is 970.02 μg / mL, the concentration of quercitrin reference substance stock solution is 30.0 μg / mL, isoquer...

Embodiment 2

[0079] Embodiment 2 blank test

[0080] Take 5 μL of 80% methanol and inject it into UPLC-MS-MS (Ultra Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometer) for determination, and record the chromatogram and multiple reaction monitoring diagram. The results showed that the solvent peak of 80% methanol aqueous solution was concentrated before 3 minutes and had no interference to the detection.

Embodiment 3

[0082] A method for assaying the content of 8 ingredients in camelina seeds, according to the steps of Example 1, the only difference is that during the preparation of the test solution in step (2), the petroleum ether extraction is changed to n-hexane extraction, and the result See Tables 5 and 6 below. Explanation of the experimental results: changing the extraction solvent did not constitute a significant difference in the measurement results.

[0083] Table 5 Determination of 8 components in camelina seeds by external standard method (ESTD) and one test multiple evaluation method (QAMS) (μg / g)

[0084]

[0085] Table 6 Determination of 8 components in camelina seeds by external standard method (ESTD) and one test multiple evaluation method (QAMS) (μg / g)

[0086]

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Abstract

The invention discloses a method for determining the contents of eight flavonoids and phenolic acid components in camelina sativa seeds, which comprises the following steps that by virtue of a separation analysis technology of an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry detector and a diode array detector and by virtue of one-detection and multi-evaluation mehod, an easily available catechin reference substance is taken as an internal reference substance, the relative correction factors between the easily available catechin reference substance and the other seven components adopted, so that the content of the eight flavonoid components in the camelina sativa seeds is measured. The method is easy to operate, high in sensitivity, accurate, efficient and low in cost, multi-index components in the camelina sativa seeds are rapidly and accurately quantified at the same time, the separation degree of the eight components is good, and a theoretical foundation is laid for quality control of the camelina sativa seeds.

Description

technical field [0001] The invention belongs to the technical field of chromatographic detection, and in particular relates to a detection method for simultaneously measuring eight flavonoids and phenolic acids in camelina seeds by using UPLC-MS-MS, thereby establishing a method for one test and multiple evaluations. Background technique [0002] Camelina sativa L. is an annual herbaceous plant, an ancient oil-bearing crop, belonging to the class Magnolia, Cincarina, Capers, Brassicaceae, and the genus Camelina. Previous studies have found that camelina seeds are rich in total flavonoids, phytosterols, unsaturated fatty acids, a variety of amino acids and trace elements, rich in essential fatty acids and a variety of natural active ingredients, the study found that the content of flavonoids in camelina seeds Higher, pharmacological studies have found that such compounds have been found to have anti-oxidation, anti-tumor, anti-cancer, anti-bacterial, immune regulation, inhibi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N2030/047
Inventor 李飞飞范毅魏悦张丽先李智宁宁二娟李自红宋梦娇张桃桃于立芹朱杰
Owner 河南省纳普生物技术有限公司
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