46 results about "Actinobacteria" patented technology

The invention discloses a microbial compound bacterial fertilizer for improving saline alkali soil and increasing the acting yield. A preparation method of the microbial compound bacterial fertilizer comprises the following steps: preparing a microbial agent containing phosphate-solubilizing bacteria, potassium-solubilizing bacteria, nitrogen-fixing bacteria, bacillus subtilis and actinomycetes; the preparation method comprises the following steps: preparing a sodium alginate solution, adding nano-hydroxyapatite and a microbial agent, uniformly mixing, dropwise adding a calcium chloride solution, and embedding an inner layer to obtain microbial embedded particles; uniformly mixing biochar, humic acid and attapulgite, taking a 2-3% HPMC solution as a binder, and carrying out middle-layer coating, so as to obtain prefabricated microbial fertilizer particles; and coating the chitosan-sodium alginate-nano ZnO outer layer to obtain the microbial compound bacterial fertilizer. The microbial compound bacterial fertilizer disclosed by the invention adopts a multi-layer and multifunctional collaborative design concept, realizes efficient protection and slow release of functional microorganisms in a saline-alkali environment, remarkably improves the physicochemical properties of soil and promotes the growth of crops.

The invention discloses a combined module for improving the yield of actinomycetes polyketone natural products and construction and application of recombinant bacteria containing the combined module, and belongs to the technical field of genetic engineering. In order to improve the yield of natural products such as polyketone, non-ribosome peptide and the like, the invention provides a combined module capable of improving the yield of actinomycetes natural products by combining and transforming a synthesis pathway of CoASH and a post-modification pathway of PPTase through a metabolic engineering strategy, and the module consists of CoASH cofactor synthesis module elements SCoaAR106A and SCoaD and a PPTase post-modification module element HPC3. By constructing a recombinant vector and recombinant bacteria containing the combined module, the yield of natural products such as actinomycetes polyketone, polyether and non-ribosomal peptide can be effectively improved by over-expression of the combined module.

The present invention relates to the field of veterinary vaccines, and discloses the development and application of a subunit vaccine for porcine contagious pleuropneumonia. The TbpB-C truncated protein and the AdhE whole protein selected in the antigen protein combination of the vaccine of the present invention are highly homologous in all APP serotypes, and are prepared into subunit vaccines that can provide cross protection to porcine actinobacillus pleuropneumoniae of different serotypes, and the protection rates against APP1 type and APP3 type strains are both 100%. The truncated proteins selected in the antigen protein combination of the present invention are successfully expressed in a soluble form after optimization, and are easy to express and purify in large quantities, thereby reducing production costs. The present invention lays a foundation for the development of an efficient, low-cost, broad-spectrum subunit vaccine for porcine contagious pleuropneumonia, and provides an effective technical means for the prevention and treatment of the disease.

The invention discloses a method for converting livestock and poultry manure into an organic fertilizer through microbial fermentation, and belongs to the technical field of livestock and poultry manure conversion. The method comprises the steps that firstly, the livestock and poultry manure is subjected to physical sorting, lime or monopotassium phosphate is added according to the pH value detection result, and the pH value is adjusted to 6.5-7.5; after determining nutrient components of the feces, adding soluble salt trace elements such as zinc sulfate and mixed carbohydrates of cane sugar and glucose; traditional strains of bacillus, lactic acid bacteria, saccharomycetes and actinomycetes are mixed with novel strains separated from the periphery of a livestock and poultry farm, optimized culture is conducted through a two-step culture method, and plant extracts such as lily extracts or livestock and poultry intestinal metabolites such as short-chain fatty acid are added in the culture process. The fermentation process adopts a rotatable fermentation tank body, and comprises three stages of fermentation of rapid heating, high-temperature stabilization and cooling decomposition. And finally, low-temperature vacuum drying, crushing, granulation and addition of a coating material are performed to prepare the organic fertilizer, so that efficient resource utilization of the livestock and poultry manure is realized.

The invention provides a preparation method and application of a biochar-based microbial inoculant, the biochar-based microbial inoculant is prepared from bacillus megatherium L2 with the preservation number of CCTCC NO: M2012381 and rice hull biochar, and the preparation method of the biochar-based microbial inoculant comprises the steps of bacillus megatherium L2 seed liquid culture and liquid fermentation, biochar proportion mixing adsorption, standing and drying. The biochar-based microbial agent prepared by the invention has a good effect of preventing and treating the tea tree anthracnose, effectively reduces the infection ability of tea tree anthracnose related pathogenic bacteria, can also effectively recruit beneficial microorganisms such as chloromentholum, actinomycelum and thick-wall mycolum in soil, further improves the prevention and treatment effect on the tea tree pathogenic bacteria, and has a good application prospect in the field of prevention and treatment of tea tree anthracnose. The method has huge application potential in protection and prevention of the tea trees and is beneficial to good growth of the tea trees.

The invention discloses a stress-resistant growth-promoting stable fertilizer and a preparation method thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer is prepared by taking soybean meal leavening, pomegranate peel residues, eucommia ulmoides leaf residues, a compound microorganism bacterium solution and a microorganism activating agent as raw materials, two high-salt-tolerance growth-promoting microorganism strains are screened to form a functional microorganism, madura anactinomyces activates the enzyme production activity of salt-tolerant bacillus by secreting metabolite, and the biological organic fertilizer is prepared. And organic matters decomposed by the salt-tolerant bacillus reversely provide a growth substrate for the madura anactinomycetes to form a metabolism reciprocal relationship. The fertilizer provided by the invention can effectively regulate and stabilize soil activity and promote crop growth, can effectively improve plant stress resistance related enzyme activity, root activity and carotenoid content in leaves under salt stress, improves resistance of soybean plants to salt stress adversity conditions, and has wide development potential and application value.

The present invention discloses a process for producing rapamycin by fermentation of actinomycetes, which comprises the following steps: 1. inoculating actinomycetes into a slant culture medium, culturing the slant culture medium, obtaining slant strains; 2. inoculating the slant strains into a primary seed culture medium, culturing the primary seeds, obtaining primary seeds; 3. inoculating the primary seeds into a secondary seed culture medium, culturing the secondary seeds, obtaining secondary seeds; 4. inoculating the secondary seeds into a basic fermentation culture medium, adding one or two of 0.1-0.5% fish meal and 0.01-0.05% alanine and 0.1-0.2% glucose after fermentation and culture for 48h or 96h, continuing fermentation and culture to the 10th day, and obtaining a fermentation liquid containing rapamycin. The present invention can synthesize more rapamycin by adding glucose, fish meal and / or alanine after fermentation and culture for a period of time, and the content can reach up to 1000μg / mL or more.

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

This invention relates to a combined module for increasing the yield of polyketide natural products from actinomycetes, and the construction and application of recombinant bacteria containing this module, belonging to the field of genetic engineering technology. To improve the yield of natural products such as polyketides and nonribosomal peptides, this invention utilizes a metabolic engineering strategy to combine and modify the CoASH synthetic pathway and the PPTase post-modification pathway, providing a combined module that can increase the yield of natural products from actinomycetes. This module consists of the CoASH cofactor synthesis module element SCoaA. R106A Composed of SCoaD and PPTase post-modification module element HPC3, recombinant vectors and recombinant bacteria containing this module were constructed. It was found that overexpression of this module can effectively increase the yield of natural products such as actinomycete polyketides, polyethers, and non-ribosomal peptides.

The invention discloses a compound microbial agent and application thereof in municipal sludge drying, and belongs to the technical field of biology. The method comprises the following steps: mixing municipal sludge and corn straw, and adjusting the water content to 60-70%; then adding a compound microorganism bacterium agent (comprising bacillus subtilis, bacillus licheniformis, common thermoactinomycetes, ureibacillus hydrotrophicus and saccharomycopsis fibuligera); and uniformly mixing the materials containing the compound microbial agent, and implanting the mixture into a fermentation tank. A certain ventilation amount is maintained through a fermentation tank system, and a pile gt is fermented in the fermentation process; the temperature can be maintained for more than 10 days at 90 DEG C, and the fermentation is finished after 10-13 days, so that the moisture content is obviously reduced, and the rapid and efficient dehydration of the municipal sludge is realized. According to the technology, rapid decomposition of municipal sludge materials can be achieved, the water content is greatly reduced, the drying period is remarkably shortened, the operation process is simple, and the technology is a microbial compound flora and technology for rapidly achieving dewatering and drying of municipal sludge.

The invention discloses a special disease-resistant compound microbial fertilizer for tea trees and a preparation method, and belongs to the technical field of agricultural fertilizers. The bacterial fertilizer consists of a microbial flora, a macro-element fertilizer, a trace element fertilizer and a carrier, wherein the microbial flora contains bacillus, trichoderma and actinomycetes. The preparation method comprises the steps of strain culture, fermentation, drying, crushing and mixing. Through the synergistic effect of various microorganisms, the bacterial fertilizer forms a protective barrier on tea tree root systems, inhibits growth of pathogenic bacteria, induces tea trees to generate resistance and enhances the disease resistance of the tea trees; the fertilizer is rich in various nutrient elements, meets the growth requirements of tea trees and promotes the growth of the tea trees; the soil structure is improved, the soil fertility is improved, and soil sustainable utilization is achieved; the content of nutritional ingredients such as tea polyphenol and amino acid in the tea can be improved, and the tea quality is improved. A plurality of embodiments verify that the bacterial fertilizer can significantly reduce the incidence rate of tea cake disease and tea anthracnose, increase the germination number and length of new shoots of tea trees, improve the amino acid content of tea leaves and the like, and has a good application effect.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The invention provides a microalgae nutrient solution and application thereof, and belongs to the technical field of agriculture. The invention provides a microalgae nutrient solution. The microalgae nutrient solution comprises nitrogen-fixing blue-green algae and photosynthetic nutrient green algae, the content of algae single cells in the microalgae nutrient solution is greater than or equal to 1.0 * 10 < 6 >. The microalgae nutrient solution provided by the invention, as a microalgae fertilizer, can significantly improve the activities of urease, sucrase and catalase in soil and reduce the activity of polyphenol oxidase in the soil; the content of alkali-hydrolyzable nitrogen in soil is increased, and the nitrogen fertilizer absorption amount of beet is improved; the richness of soil microbial communities can be increased, and the number of actinomycetes and proteobacteria is increased; growth and development of the beet can be remarkably promoted, the plant height, fresh weight, leaf number, chlorophyll content and net photosynthetic rate of the beet can be improved, accumulation of dry matter of beet tuberous roots can be promoted, timely transfer of beet growth centers can be regulated, and then the yield and sugar yield of the beet can be increased.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes CoT10, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The process disclosed by the invention can produce spores on a large scale, can shorten the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; meanwhile, the obtained spores can be freeze-dried to form spore powder under the protection action of trehalose, cane sugar and a sodium glutamate solution, so that the survival rate and the stability of the spores in long-term storage can be improved. The spore provided by the invention can be used for relieving heavy metal stress of plants and promoting plant growth, is more environment-friendly to ecology when being used for preparing a microbial fertilizer, does not influence an agricultural ecological environment and human body health, can promote growth and development of host plants, and is high in use value and good in application prospect.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The invention discloses a veterinary medicine composition, which is prepared from the following medicines in parts by weight: 6 to 13 parts of penicillin, 0.5 to 1.4 parts of herba violae extract and 0.5 to 1.4 parts of fish extract. Through scientific proportioning of penicillin, a Corydalis delavayi Franch. Extract, a fish extract and other ingredients, the action mechanism of the penicillin is to inhibit synthesis of bacterial cell walls, so that the purpose of killing bacteria is achieved. The penicillin has a strong antibacterial effect on gram-positive bacteria, and especially has a good curative effect on most diseases caused by gram-positive bacteria, such as respiratory system infection, swine erysipelas, anthrax, septicemia and the like. Besides, penicillin also has a strong antibacterial effect on various spirochetes and actinomycetes, the delavay schefflera leaves can promote blood circulation, relieve swelling and pain, resist bacteria and kill viruses and enhance immunity, and meanwhile, the penicillin and the delavay schefflera leaves are matched with the fish extract, so that an effective treatment effect can be achieved.

The invention discloses a one-time fertilization method for rice, and particularly relates to the technical field of one-time fertilization methods for rice, which comprises the following steps: S1, plowing a rice field; s2, frequent shallow water irrigation; s3, an early growth stage after seedling throwing; s4, a fertilization process; s5, rice growth middle and later periods. According to the one-time fertilization method for the rice, soil can be improved, activated carbon can be supplemented for the soil, soil hardening is broken, acidification is inhibited, and degeneration is prevented. Organic and inorganic pollution residues in the soil can be effectively removed, and particularly, an obvious degradation effect on heavy metal cadmium pollution is achieved. If the compound fertilizer is continuously used for 2 years, the quantity of soil actinomycetes can be increased by 28 times, the quantity of nitrogen-fixing bacteria can be increased by 34 times, a good soil environment is created for rice growth, root development and nutrient absorption are facilitated, the yield of food crops can be increased by 15-20%, the yield of commercial crops can be increased by 30-50%, and the product quality is improved by 1-3 grades. After the fertilizer is applied to the late rice field, the yield is obviously higher than that of a contrast field, the rice cadmium content is greatly reduced, and the quality is improved while the yield is increased.

The invention belongs to the technical field of microbial pesticides, and particularly relates to acidophilic actinomycetes Actinacdiphila reveromyces 3-10 and application of the acidophilic actinomycetes 3-10 in prevention and treatment of plant diseases. The technical problem to be solved by the invention is to provide a new choice for biological control of plant diseases. According to the technical scheme, the acidophilic actinomycete is acidophilic actinomycete 3-10, and the preservation number of the acidophilic actinomycete 3-10 is CCTCC (China Center for Type Culture Collection) NO: M 20242102. The invention also provides an application of the acidophilic actinomycetes Actinacdiphila reveromyces 3-10 in the prevention and treatment of plant diseases, and the acidophilic actinomycetes Actinacdiphila reveromyces 3-10 can be applied to the prevention and treatment of plant diseases. According to the acidophilic actinomycete, the fact that the fermentation liquor of the acidophilic actinomycete 3-10 can be used for effectively preventing and treating plant epidemic diseases, damping-off and fusarium wilt is found for the first time.

The invention belongs to the technical field of slow-release fertilizer preparation, in particular to a bio-energy-accumulating and synergistic slow-release fertilizer and a preparation process thereof. Aiming at the problem that it is difficult for the existing slow-release fertilizer to completely match the fertilizer absorption law of crops in different growth and development periods in nutrient release, resulting in insufficient nutrient supply of crops in certain periods and inability to effectively improve soil structure and environment, the following scheme is proposed, which includes raw materials, and the raw materials include the following components in parts by weight: 20-80 parts of polyvinyl alcohol. The invention can effectively improve soil structure and environment and significantly improve fertilizer utilization rate by using polyvinyl alcohol, polyethylene, polyamide, polyurea, paraffin, polyglutamic acid, zinc polyaspartate, nitrification inhibitor, urease, fulvic acid, humus, amino acid, actinomycetes, spirochetes, ammonium magnesium phosphate, polypropylene, polyvinyl chloride, urea phosphate, talcum powder, sulfur, biochar, zeolite and diatomaceous earth.

The invention discloses a method for preparing an amino acid-containing water-soluble fertilizer from a fox racoon dog carcass. The method comprises the following steps: cutting and crushing the fox racoon dog carcass to obtain meat paste; mixing the meat paste with a microbial agent according to a material-liquid ratio of 1.8: 1; the microbial agent comprises thermoactinomycetes, bacillus subtilis and bacillus licheniformis which are mixed according to the volume ratio of 1: 1.3: 2, an enzyme mixture is added into the meat paste according to the mass ratio of 4: 1, an oxygen producer is added into the meat paste until the meat paste cannot be seen by naked eyes, the reaction is ended, oil-water separation is conducted after filtering, and an oil-water separation product is obtained; and performing standard regulation on the deoiled liquid to obtain the amino acid-containing water-soluble fertilizer. According to the method, thorough decomposition of the carcasses is achieved through the compatible microbial agents, the filtered sediment can be decomposed repeatedly, no solid waste is generated, environmental pollution is avoided, and a safe and traceable recycling way is provided for the fox raccoon dog carcasses.

The invention discloses a microbial agent for preventing diseases and promoting growth as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent disclosed by the invention comprises bacillus aryabhattai, pseudomonas oryzae and actinoplanes dark orange. Bacillus aryabhattai, pseudomonas oryzae and actinoplanes darkorange are screened to form functional microorganisms, and after the three bacterial liquids are mixed in equal proportion, various antibacterial substances are generated to synergistically inhibit the growth of pathogenic bacteria, and the bacillus aryabhattai, pseudomonas oryzae and actinoplanes darkorange have good antagonistic effects on soybean root rot, soybean anthracnose and soybean gray speck disease. The microbial agent provided by the invention can promote soybean growth, increase soybean yield, reduce the occurrence probability of soybean root rot, soybean anthracnose and soybean gray speck disease, solve the problems of low yield and high incidence of diseases of part of soybeans, is safe and environment-friendly in components, is non-toxic to soybeans, and has a positive effect on reducing environmental pollution and pesticide residues.

The present invention provides a biological preparation for porcine Actinobacillus pleuropneumoniae disease, belonging to the technical field of porcine Actinobacillus pleuropneumoniae disease treatment. The present invention finds that Inula grandiflora can effectively inhibit Porcine Actinobacillus pleuropneumoniae, and finds that it can break the biofilm of Porcine Actinobacillus pleuropneumoniae and inhibit the adhesion of Porcine Actinobacillus pleuropneumoniae to porcine lung epithelial cells. Therefore, Inula grandiflora can be prepared into a biological preparation for treating Porcine Actinobacillus pleuropneumoniae disease.

The invention discloses a method and application of a synthetic microbial inoculum for straw degradation in acidic red soil. The synthetic microbial inoculum comprises bacteria B and actinomycetes C, the bacterium B is Rhodococcus erythropolis, the strain Straw-P2F9 is preserved in the China General Microbiological Culture Collection Center on January 20, 2025, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.33465; the actinomycete C is Paenarthrobacter nitroguajalicus, the strain of the actinomycete C is Straw-B50, the strain of the actinomycete C is preserved in the China General Microbiological Culture Collection Center on January 20, 2025, and the preservation number of the actinomycete C is CGMCC No. 33463. The synthetic bacterial agent improves the degradation rate of straw in acidic red soil, and improves the yield and quality of crops.

The invention provides a method for screening and verifying circMKLN1 circular RNA (Ribonucleic Acid) capable of regulating and controlling triple negative breast cancer, circMKLN1 is formed by transcription and reverse splicing cyclization of 4-7 exons of an MKLN1 gene, and the length of circMKLN1 is 535bp. The primer of the circMKLN1 is used for carrying out PCR (Polymerase Chain Reaction) amplification and then is sent to a living organism company for Sanger sequencing, and the result shows that the specific splicing site of the circMKLN1 is found. The cDNA and the gDNA are used as templates, PCR amplification is carried out by using a circMKLN1 convergence primer and a circMKLN1 divergent primer, and the result shows that the circMKLN1 divergent primer can only be amplified in the cDNA and is not amplified in the gDNA, so that the existence of the circMKLN1 is further proved. The RNase R and actinomycin D experiments show that the circMKLN1 has higher resistance to the RNase R, and the half-life period of the circMKLN1 is far longer than the half-life period of linear MKLN1 mRNA (messenger ribonucleic acid). A nucleoplasm separation experiment shows that circMKLN1 mainly exists in cytoplasm.

This invention discloses an S-type α-fluoroimine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from actinomycetes. Actinomadura welshii By using directed evolution to modify its active site, S-type α-fluoroimine reductase mutants with high activity, stereoselectivity, and high yield of 4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline were obtained. These mutants were D98G, D98G / M122L, or D98G / M122L / W216V. This invention achieves efficient synthesis of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline from inexpensive (trans)-2,2,2-trifluoro-N-(4-methoxyphenyl)-1-phenylethylimine, which is beneficial for the industrial synthesis and application of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline.

The present disclosure features compositions and methods for the treatment of actinomycetia (e.g., corynebacteriales) infections, e.g., caused by mycobacterial cells. In one aspect, the disclosure features a composition containing unencapsulated proteins that include one or more of (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an α-amylase. The composition optionally further includes a supramolecular structure including one or more (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an α-amylase. In another aspect, the invention features a method of treating a bacterial infection in a subject by administering a composition of unencapsulated lytic proteins as described herein to the subject in an amount and for a duration sufficient to treat the bacterial infection, optionally in combination with encapsulated lytic proteins. The encapsulated lytic proteins and the unencapsulated lytic proteins can be in the same or different composition and can be administered simultaneously or sequentially.

The application discloses a kind of silkworm excrement carrier fixed pseudomonas aeruginosa PS1 biological agent and application thereof.Pseudomonas aeruginosa PS1 has strong degradation ability to PAEs, the degradation rate of DBP reaches 97.5%, the degradation rate of DEHP reaches 60.2%, and can use catechol, BA, PCA, PA, MBP, benzoic acid monobutyl ester, 4,5-dihydroxy phthalic acid, DEP, DMP and DOP and other various PAEs as substrate.The silkworm excrement is used as the carrier of fixed pseudomonas aeruginosa PS1, and the biological agent for repairing PAEs contaminated environment is prepared, is used to significantly improve soil pH, increase soil organic matter content and / or increase soil nitrate nitrogen and / or ammonium nitrogen content, increase the abundance of phylum Proteobacteria and / or phylum Synergistetes, and / or reduce the abundance of actinomycetales and / or Pseudonocardiales, so as to repair the environment contaminated by PAEs.

The invention provides a soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of resource utilization and biological energy sources. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 20 to 40 parts of coffee grounds, 10 to 30 parts of charcoal, 5 to 15 parts of bentonite, 3 to 10 parts of small molecular organic acid, 1 to 5 parts of metal ion chelating agent, 15 to 25 parts of organic fertilizer, 0.5 to 3 parts of microbial flora and 2 to 8 parts of humic acid; wherein the microbial bacteria are mixed bacteria and simultaneously comprise bacillus amyloliquefaciens, rhizosphere kocuria and salt-tolerant actinomycetes. According to the soil conditioner, the raw materials and the proportion of the conditioner are optimized, so that all the components have a synergistic effect, the improvement effect of the saline alkali soil is effectively improved, and the improvement process is accelerated.