169 results about "Actinobacteria" patented technology

The invention relates to an inorganic-organic microbial compound fertilizer and a preparation method thereof. The inorganic-organic microbial compound fertilizer is characterized by comprising ordinary high-concentration three-element compound fertilizer, humic acid, fly ash, microbial compound bacteria powder and other elements, wherein the microbial compound bacterial powder is obtained by mixing bacterial powders which are prepared by respectively fermenting and culturing photosynthetic bacteria, yeast, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, bacilluslaterosporus, bacillus megaterium, bacillus mucilaginosus, azotbacteria chroococum and actinomycetes. In the inorganic-organic microbial compound fertilizer, the microbial content of the yeast is no less than 2.0*108cfu/g, and the microbial contents of the other strains are all no less than 2.0*109cfu/g. The inorganic-organic microbial compound fertilizer can centralize the advantages of an organic fertilizer, an inorganic fertilizer and microorganisms, thereby enhancing the stress resistance and the disease resistance of crops, enhancing the fertilizer absorption ability of the crops, lowering the soil hardening degree and improving the soil ecological environment and the quality of the crops.

The invention discloses a functional microorganism decomposing agent and a preparation method and application thereof. The functional microorganism decomposing agent is prepared from, by weight, 30%-60% of bacteria, 20%-50% of fungi and 20%-50% of actinomycetes, wherein the effective living bacteria count of each of the bacteria, the fungi and the actinomycetes is more than or equal to 50 millions per gram of the decomposing agent, and the total effective living bacteria count of the bacteria, the fungi and the antinomycetes is more than or equal to 200 millions per gram of the decomposing agent. According to the functional microorganism decomposing agent and the preparation method and application thereof, the production technology is simple, all the bacterial strains are complete and reasonable in proportion, and the decomposing agent is high in decomposing and degrading speed and good in using effect.

The invention discloses a cultivation method for improving corn yield in saline-alkali land. The method comprises the following steps: irrigating the saline-alkali land for flooding for once in the middle of March, wherein the irrigation volume for per mu is 60-100 m<3>, airing for 1-1.5 months until the surface of earth is dry enough to allow people to step on, applying 1000kg-1500kg of miscellaneous manure or 50kg-55kg of compound fertilizer per mu, then performing rotary tillage for 1-2 times to mix soil and the fertilizer, pressing by using a harrow and preserving soil moisture, and leveling the surface of earth; treating corn by using a seed coating agent before the corn is seeded, wherein the seed coating agent comprises actinomycin, bacillus mucilaginosus, azospirillum, diatomite, spicy powder, heteroauxin, potassium sulphate, magnesium sulfate, zinc sulfate, an alpha-pimacol growth regulator, an EDTA-2Na chelating agent, humic acid and turf; mixing bactericide and insecticide with seeds for treating the seeds, namely, firstly adding the insecticide, and then adding the bactericide after piling for 3-6 hours; and immediately seeding after the seed mixing treatment is finished.

The invention discloses a plant enzyme foliar fertilizer and a preparation method thereof, relating to the technical field of plant foliar fertilizers. The formula of the enzyme foliar fertilizer comprises the following components in parts by weight: 100 parts of boiled water, 70 parts of brown sugar, 4 parts of soybean, 3 parts of enzyme enlargement bacterium, 1 part of vitamin and 1 part of microelement. The invention perfectly combines organic nutrients and inorganic nutrients, has special effect on the growth, the development, the disease prevention, the pest control, the yield increase and the quality improvement of crops, cannot damage the soil structure of the soil, cannot influence the nutrient components of the crops and makes the crops green. Meanwhile, beneficial microbes like actinomycetes and the like contained in the plant enzyme foliar fertilizer can effectively restrain foliage diseases and reduces the morbidity of the crops.

The invention relates to an organic liquid fertilizer containing chitosan. The organic liquid fertilizer comprises the chitosan, humic acid, fulvic acid, phosphorus pentoxide, potassium hydroxide, boric acid, edetic acid, zinc sulphate, manganese sulfate monohydrate, glycine, glutamic acid, serine and lysine. Aqueous extract of traditional Chinese medicines is added into the organic liquid fertilizer. As various marine bioactive ingredients such as the chitosan are added into the organic liquid fertilizer, the organic liquid fertilizer can be used for culturing a great quantity of effective microbial communities of actinomycetes and the like within short time after being applied to soil. Antibiotics and other substances secreted from the effective microbial communities can inhibit harmful microbial communities of pythium aphanidermatum, rhizoctonia and the like, and thus a microbial environment of the soil can be improved rapidly. The chitosan also can be used for accelerating the growth of root systems and the combination of the root systems and the soil. In addition, as the aqueous extract with insecticidal efficacy of the traditional Chinese medicines is added into the organic liquid fertilizer, plant diseases and insect pests of leaf surfaces can be effectively reduced when the fertilizer is applied to the leaf surfaces.

The invention discloses a method for using waste molasses of sugar factory to produce biological leaf fertilizer, which comprises disinfecting a fermentation vessel via boiled water, boiling waste molasses to kill bacterials and grafting microbe strains as aerobic bacteria, yeast and actinomycetes at 35DEG, preparing fermentation materials as 20-30kg of waste molasses, 0.3-1kg of grafting microbe and 20-30kg of sterile water, arranging the fermentation material into a fermentation vessel, mixing uniformly via a disinfection rod, covering 2-3 gauze layers on the mouth of the vessel, culturing and fermenting, starting aeration each day from the fourth day as 30min at morning and 30min at afternoon until there is dense wine aroma. The invention uses aerobic bacteria, yeast and actinomycetes as grafting microbe strains to process mixed fermentation at suitable temperature, facultative and aerobic conditions to convert organics as sugar into useful compositions as glucose, alcohol, amino acid, nucleic acid and vitamin B.

Disclosed are a fertilizer for advancing alkaline land and its preparing method, comprising of microbe compound bacterium solution and dry matter and their mess ratio is 1:80-150, the microbe compound bacterium solution is formed by plant lactobacillaceae, distillers yeast . The invention is made by completely using organic substance to fermen, which can adyance alkaline land as wellas be fertilizer for green organic agricultural products.

The invention relates to marine streptomyces S187 having broad-spectrum antibacterial activity, belonging to the microbiological actinomycetes field. The marine streptomyces S187 is separated from a sea mud sample at a depth of 20 meters underwater in coastal waters. Primary screening through an agar block method proves that the bacterial strain has very strong antibacterial activity to Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger; the result of fermentation liquid secondary screening confirms good activity for antagonizing the Escherichia coli, the Staphylococcus aureus and the Bacillus subtilis. The analysis for 16S rDNA shows that the only actinomycetes having the homology as high as 99 percent with the marine streptomyces S187 is the salt lake bacterial strain Streptomyces chungwhensis (DSM41483) separated by Korean scholars, but the bacterial strain is separated from the sea mud sample for the first time. I type polyketide synthase (PKSI) is successfully amplified out from the streptomyces S187, which indicates that the streptomyces S187 has the potential ability of generating polyketide.

The invention provides a microbiological composite bacterial agent and an application thereof in adjusting soil pH value. The bacterial agent comprises the following active components: bacillus, lactobacillus, saccharomycetes, soil actinomycetes, and mycete. The bacterial agent can adjust soil pH value, establishes a good crop rhizosphere micro-ecological environment, loosens the soil, increases the air permeability, improves the water-retention and fertilizer conservation performance of the soil, helps to form dominant bacterial communities of beneficial bacteria, effectively inhibits the growth of pathogenic microorganism, relieves the soil borne diseases, strengthens the anti-disease performance of crops, maintains the soil micro-ecological system and ecological balance, modulates the soil structure, improves the crop rhizosphere soil micro-ecological system, promotes the crop to grow roots, increases the crop output, improves the product quality, increases the microorganism content and trace element content in soil, and thus achieves the goals of improving physical and chemical properties of soil, strengthening the water-retention and fertilizer conservation performance of the soil, and increasing the air permeability of the soil. The microbiological composite bacterial agent has the advantages of user-friendliness, low cost, and prominent effect, can prevent the happening of insects and diseases, and improves the product output and quality.

The invention discloses an organic base fertilizer special for grapes and a production process thereof. The organic base fertilizer special for grapes contains the following components: bean cakes, sheep manures, biogas slurry, Sophora alopecuroide branch and leaf slurry, rice bran oil residues, organic phosphorus, fulvic acid, amino acid magnesium, microelements and beneficial biological species solution. The production process of organic base fertilizer includes: 1 bean cakes, sheep manures and rice bran oil residues are crushed to be powder, green and tender branches and leaves of Sophora alopecuroide are struck into slurry, magnesium oxide and potassium hydroxide are added into fulvic acid saturated solution to synthesize amino acid magnesium and fulvic acid potassium, the seven microelements are prepared into saturated solution, and three beneficial biological bacteria such as enzyme bacteria, nitrogen-fixing bacteria and actinobacteria are cultured for 7-10 days to be high-concentration bacterium liquid; and 2 the components are evenly mixed together in proportion and ferment for 15-20 days to become the fertilizer. The organic base fertilizer special for grapes can promote fruits to expand fast, continuously improve sugar content, improve the grape yield by 8-10% and the sugar content by 3-4%, and enable the grapes to store for a long time and carry conveniently.

The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.

The invention relates to a method for preparing compost by washing wool junk microbial fermentation. Microorganism accounting for 10 percent of the total mass of silt is implanted in the washing wool silt and fermented for 15-30 days at the temperature of 45-65 DEG C. The microorganisms are the composite of bacteria, fungi and actinomycetes, and the number ratio of the bacteria, the fungi and the actinomycetes is 10-30:0.5-2.5:0-1.5. The bacteria are bacillus subtilis, Pseudomonas aeruginosa and Bacillus mucilaginosus with the number ratio of 1-5:0.5-3:0.3-1.5; and the fungi are Aspergillus niger and Penicillium simplicissimum with the number ratio of 1-3:0.5-1.5. The compost produced by fermentation contains 30-80% of organic matter, 3-15% of total nitrogen, 2-10% of total phosphorus and 1-7% of total potassium.

The invention belongs to the technical field of wastewater treatment and particularly relates to a microbial agent suitable for treating urban wastewater and a preparation method thereof. The microbial agent comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, saccharomycetes, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria, Rhodospirillum, Thiobacillus denitrificans, Sphingobacteria, Alcaligenes faecalis, Bifidobacterium, brown rot fungi,Geotrichum candidum, Mucor, Aspergillus oryzae, and Penicillium diversum. The preparation method includes: mixing well Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria,phosphorus-accumulating bacteria and Bifidobacterium, adding the mixture into clean soil, farming earthworms with the soil for 12-14 days, and isolating the earthworms and the soil to obtain earthwormcast containing the mixed bacteria; mixing the earthworm cast containing the mixed bacteria and other bacteria, and immobilizing to obtain the microbial agent.

The invention belongs to the technical field of biological bacterial fertilizers and particularly relates to a dendrobium officinale complex bacterial agent. The complex bacterial agent is prepared by steps of separation and purification of in-root actinomycetes, screening of antagonistic actinomycetes, screening of mycorrhiza fungi growth promoting actinomycetes, determination of species status of the growth promoting actinomycetes and growth promotion of dendrobium officinale clonal seedlings by synergy of optimized mycorrhiza fungi and the actinomycetes. By synergy of dendrobium officinale mycorrhiza fungi and the in-root actinomycetes for obtaining the complex bacterial agent, dendrobium officinale cells can be stimulated to generate efficient defense capability, self repairing, disease resistance, extreme environment resistance of dendrobium officinale can be improved, the survival rate of the dendrobium officinale clonal seedlings is increased, growth of the dendrobium officinale clonal seedlings is promoted, and large-scale production of high-quality dendrobium officinale seedlings is supported technically.

The invention discloses an actinomycete strain JSX-1 for preventing and curing phytophthora sojae, and belongs to the technical field of plant protection. The strain belongs to streptomycete (Streptomyces sp.) and is named as actinomycete JSX-1. The single colony of the actinomycete strain is circular, white and dry and non-transparent in surface; the hypha is thinner, and grows slowly; the bacterial colony is compact in texture and has a fishy smell. The gram stain of the actinomycete strain is purple, and positive. The JSX-1 fungicide has the controlling effect of 55 to 60 percent on phytophthora sojae. The invention provides the actinomycete strain JSX-1 for preventing and curing phytophthora sojae and the fungicide thereof which are not liable to enable phytophthora sojae to produce resistance, and has no pollution to environment, and the fungicide can be used through root-irrigation after soybean seedling emergence.

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

The invention discloses a method for extracting gutta-percha from the leaves and bark of eucommia ulmoides. The method comprises the steps that the leaves and bark of the eucommia ulmoides are washed, dried and mechanically crushed, soaking is carried out with aqueous alkali at normal temperature, filtering is carried out, and filter residues are subjected to high temperature sterilization; an aqueous surfactant solution is added to adjust the moisture content of the filter residues, then the filter residues are inoculated with actinomycetes strains, a carbon source and a nitrogen source are added, and fermentation is carried out to damage cellulose in the leaves and bark of the eucommia ulmoides; then the gutta-percha is extracted through a conventional solvent method. Through aqueous alkali pretreatment on the gutta-percha, the leaves and bark of the eucommia ulmoides are fermented through actinomycetes in an alkali environment to degrade lignin, meanwhile, and the aqueous surfactant solution is added to improve the degradation efficiency of the actinomycetes on the cellulose in the leaves and bark of the eucommia ulmoides. According to the method, the gutta-percha yield is increased, and meanwhile the efficiency of extracting the gutta-percha through a biodegradation method is improved.

The invention is related to a method and DNA constructs for obtaining in a filamentous fungus host a higher production level of a carbohydrate degrading (CD) enzyme, having in its original state a catalytic module (CAT) and a carbohydrate binding module (CBM) separated by a linker region. The DNA construct comprising a truncated actinomycetes, preferably Nonomuraea flexuosa (NJ) derived DNA sequence encoding a truncated form of the CD enzyme, for example Nf Xyn11A, Nf Xyn10A, and is introduced into a filamentous fungal host. Said CD enzyme contains the catalytically active region of CAT but lacks part or all of the CBM, or all of the CBM and part or all of the linker region and is expressed and secreted under the control of regulatory sequences comprising at least a signal sequence, but also promoters, terminators and DNA sequences encoding a secretable carrier protein or domains thereof, preferably originating from filamentous fungi are included. The production level obtained with DNA sequence having the shortened DNA sequence encoding the truncated form of the CD enzyme is higher than the production level obtained with DNA construct encoding the corresponding full length CD enzyme.

The present invention discloses actinomycetes having a Phytophthora melonis inhibition effect, and a screening method thereof, wherein the actinomycetes are a Streptomyces virginiae X54 strain with a preservation number of CGMCC No.10042, and are preserved in the China General Microbiological Culture Collection Center. According to the present invention, the actinomycetes do not produce pathogenicity to humans and animals, are antagonistic actinomycetes capable of preventing and controlling Phytophthora melonis in a safe, non-toxic and effective manner, provide a certain inhibition effect for hot pepper anthracnose bacteria, tobacco black shank bacteria, rice sheath blight disease bacteria, wheat scab bacteria, citrus anthracnose bacteria, and cucumber fusarium wilt bacteria, and especially provide great development potential in prevention and control of cucumber blight.

The invention discloses a microecological modulator. The microecological modulator is prepared from photosynthetic bacteria, actinomycetes, bacillus, saccharomycetes, lactic acid bacteria, nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium bacteria, silicate bacteria, bacillus radicicola, blue-green algae, vitamin C, nitrogen, boron, iron, saccharose, calcium sulfate, dipotassium phosphate, calcium carbonate, magnesium sulfate, sodium chloride and agar. According to the microecological modulator disclosed by the invention, by adopting a fusion technique of multiple useful microbiological populations, the useful microbiological population and plant enzymes are fermented and cultivated at the same time, mutual phagocytosis of biological populations in a fusion process of the biological populations can be prevented, and symbiosis and compatibility are realized. The microecological modulator disclosed by the invention is rich in multiple microbiological populations and multiple trace elements and mineral substances needed by plant growth, so that microecological circulation of soil can be improved, and the fertilizer efficiency of the soil is enhanced; the microecological modulator replaces chemical fertilizer; photosynthesis of leaf surfaces of plants and crops is enhanced, the oxidation is reduced, and leaf surfaces of the plants and the crops can be lustrous and plump; the plants and the crops can be full in fruits; the yields of the plants and the crops can be increased.

The invention discloses angucycline compounds and an application of the angucycline compounds in preparation of an anti-tumour or antibacterial medicine. According to the invention, fermentation extract of marine actinomycete, namely streptomyces rubrogriseus SCSIO 11594, is separated and purified, three cytotoxic active or bacteriostatic active compounds are obtained, and the three cytotoxic active or bacteriostatic active compounds are determined to be angucycline compounds through structural analysis, and a concrete structure is shown in a formula (II) in the specification. The three compounds 1, 2 and 3 are separated from streptomyces rubrogriseus SCSIO 11594, wherein the compounds 2 and 3 have anti-tumour activity and can be used for preparing an anti-tumour medicine, and the compounds 1, 2 and 3 have antibacterial activity and can be used for preparing an antibacterial medicine, so that the angucycline compounds disclosed by the invention provide new or new-functional pilot compounds for development of new anti-tumour or antibacterial medicines and have important significance for developing China marine medicinal resources.

The invention discloses a microorganism environment modifier and a microorganism packing material. The modifier comprises photosynthetic bacteria, acetobacter aceti, yeasts, lactic acid bacteria, actinomycetes and filamentous bacteria. The invention also discloses the microorganism packing material. The packing material comprises a packing raw material and the modifier. The modifier comprises the following bacterial floras in percentage: 20 percent to 30 percent of photosynthetic bacteria, 2 percent to 10 percent of acetobacter aceti, 20 percent to 30 percent of lactic acid bacteria, 10 percent to 20 percent of yeasts, 10 percent to 20 percent of actinomycetes and 10 percent to 20 percent of filamentous bacteria. By the modifier and the packing material, the immunologic function of an animal can be improved, the environment is purified, and the healthy growth of the animal is promoted. The microorganism environment modifier and the microorganism packing material are suitable for breeding various domestic animals. During breeding, a strain maintenance packing material does not need to be added. After the service lives of the microorganism environment modifier and the microorganism packing material are reached, the microorganism environment modifier and the microorganism packing material can also be used for planting vegetable crops.

The invention provides a microbial agent. The microbial agent comprises the following components in parts by weight: 20 to 60 parts of hydrogen-oxidizing bacteria, 20 to 60 parts of nitrogen-fixing bacteria, 30 to 50 parts of actinomycetes and 30 to 60 parts of fungi, wherein the actinomycetes can be actinoplanes, streptomycetes or nocardia; and the fungi can be pythium or trichoderma. The microbial agent disclosed by the invention generates a certain synergistic effect through the combination of the hydrogen-oxidizing bacteria, the nitrogen-fixing bacteria, the actinomycetes and the fungi, and can improve the yield of wheat, promote the development of wheat root systems and improve the good earth holding capacity of roots for soil, so that main roots, side roots and fibrous roots are deeper and wider in coverage and are difficult to lodge and stronger in water absorption, eustipes close to the ground has better toughness and strength, the toughness and strength of the stems can be improved, and the stems are difficult to break. The microbial agent provided by the invention can shorten the growth and maturation time, enable crops to be matured in advance, reduce the risks of vigorous growth of seedlings and green remaining and late maturation of plants, and promote early fruiting and maturation of the plants to appear in the market.

The invention relates to a method for verifying and fermenting polar marine ray fungi AFN1007 producing antitumor active substance, and the research on the antitumor activity of the zymotic fluid thereof, belonging to the microbial medicine field. The strain AFN1007 is verified to be a novel kind of Streptomyces by the traditional classification and the analysis of 16S rDNA gene of the AFN1007. The compositions of a culture medium by percentage are: 1 percent of soybean flour, 1 percent of tragantine, 0.1 percent of KNO3, 0.05 percent of MgSO4, 0.001 percent of FeSO4, 0.05 percent of NaCl and natural seawater with a pH value between 7.2 and 7.4. The strain is fermented by using the culture medium. The MTT method shows that the zymotic fluid has strong antitumor action on four tumor cells of KB, BGC 803, Hep G2 and Hela and an antitumor compound with a novel structure is expected to be found in the zymotic fluid.

The invention discloses a thermophile complex inoculant and a method thereof for degrading excess sludge. The thermophile complex inoculant is prepared by uniformly mixing five thermophiles, namely, bacillus stearothermophilus, geobacillus sp, bacillus pumilus, bacillus subtilis and actinomycetes at the ratio of 3 to 3 to 3 to 2 to 2, wherein the five thermophiles are obtained by separating from soil, a petroleum pollution sample, composted plant straws and composted sludge, screening and identifying. The thermophile complex inoculant, under certain conditions, can degrade 70% of excess sludge after 5d fermentation, and can completely degrade the excess sludge within 10d. With the application of the thermophile complex inoculant, excess sludge composting efficiency can be improved and degradation of organic matters can be accelerated; therefore, the thermophile complex inoculant takes an important role in excess sludge treatment and recycling. The method is convenient to operate, relatively good in economic performance and easy for management; and the method shows a good application potential.

The invention discloses a biosynthetic gene cluster of big-ring lactam compound heronamides and application thereof. The nucleotide sequence of the biosynthetic gene cluster of the heronamides derived from deep-sea actinomycetes Streptomyces sp. SCSIO 03032 (with the preservation number being CCTCC NO. M 2011258) is as shown as the 2662-82562th bit of the SEQ ID NO.1 and comprises 20 genes. The nucleotide sequence of the P450 oxidase gene HerO is shown as the base sequence of the 24299-25546th bit of the SEQ ID NO.1. The P450 oxidase gene HerO can catalyze heronamide G to form heronamide F. The genetically engineered bacterium without the P450 oxidase gene HerO can be used for preparing heronamide G. According to the heromanides, biosynthesis is conducted on all the related gene and protein information, the biological synthesis mechanism of twenty-element ring big-ring lactam compound is explained beneficially, structural analogues of the heromanides can be obtained through reformation of the biosynthetic genes, and meanwhile the biosynthetic gene cluster can be used for searching for and excavating compound or gene protein capable of being used for medicine and health care and industries and agriculture in the nature.