20 results about "Actinobacteria" patented technology

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

This invention relates to a combined module for increasing the yield of polyketide natural products from actinomycetes, and the construction and application of recombinant bacteria containing this module, belonging to the field of genetic engineering technology. To improve the yield of natural products such as polyketides and nonribosomal peptides, this invention utilizes a metabolic engineering strategy to combine and modify the CoASH synthetic pathway and the PPTase post-modification pathway, providing a combined module that can increase the yield of natural products from actinomycetes. This module consists of the CoASH cofactor synthesis module element SCoaA. R106A Composed of SCoaD and PPTase post-modification module element HPC3, recombinant vectors and recombinant bacteria containing this module were constructed. It was found that overexpression of this module can effectively increase the yield of natural products such as actinomycete polyketides, polyethers, and non-ribosomal peptides.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes CoT10, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The process disclosed by the invention can produce spores on a large scale, can shorten the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; meanwhile, the obtained spores can be freeze-dried to form spore powder under the protection action of trehalose, cane sugar and a sodium glutamate solution, so that the survival rate and the stability of the spores in long-term storage can be improved. The spore provided by the invention can be used for relieving heavy metal stress of plants and promoting plant growth, is more environment-friendly to ecology when being used for preparing a microbial fertilizer, does not influence an agricultural ecological environment and human body health, can promote growth and development of host plants, and is high in use value and good in application prospect.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The invention discloses a method for preparing an amino acid-containing water-soluble fertilizer from a fox racoon dog carcass. The method comprises the following steps: cutting and crushing the fox racoon dog carcass to obtain meat paste; mixing the meat paste with a microbial agent according to a material-liquid ratio of 1.8: 1; the microbial agent comprises thermoactinomycetes, bacillus subtilis and bacillus licheniformis which are mixed according to the volume ratio of 1: 1.3: 2, an enzyme mixture is added into the meat paste according to the mass ratio of 4: 1, an oxygen producer is added into the meat paste until the meat paste cannot be seen by naked eyes, the reaction is ended, oil-water separation is conducted after filtering, and an oil-water separation product is obtained; and performing standard regulation on the deoiled liquid to obtain the amino acid-containing water-soluble fertilizer. According to the method, thorough decomposition of the carcasses is achieved through the compatible microbial agents, the filtered sediment can be decomposed repeatedly, no solid waste is generated, environmental pollution is avoided, and a safe and traceable recycling way is provided for the fox raccoon dog carcasses.

This invention discloses an S-type α-fluoroimine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from actinomycetes. Actinomadura welshii By using directed evolution to modify its active site, S-type α-fluoroimine reductase mutants with high activity, stereoselectivity, and high yield of 4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline were obtained. These mutants were D98G, D98G / M122L, or D98G / M122L / W216V. This invention achieves efficient synthesis of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline from inexpensive (trans)-2,2,2-trifluoro-N-(4-methoxyphenyl)-1-phenylethylimine, which is beneficial for the industrial synthesis and application of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline.

The present disclosure features compositions and methods for the treatment of actinomycetia (e.g., corynebacteriales) infections, e.g., caused by mycobacterial cells. In one aspect, the disclosure features a composition containing unencapsulated proteins that include one or more of (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an α-amylase. The composition optionally further includes a supramolecular structure including one or more (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an α-amylase. In another aspect, the invention features a method of treating a bacterial infection in a subject by administering a composition of unencapsulated lytic proteins as described herein to the subject in an amount and for a duration sufficient to treat the bacterial infection, optionally in combination with encapsulated lytic proteins. The encapsulated lytic proteins and the unencapsulated lytic proteins can be in the same or different composition and can be administered simultaneously or sequentially.

The application discloses a kind of silkworm excrement carrier fixed pseudomonas aeruginosa PS1 biological agent and application thereof.Pseudomonas aeruginosa PS1 has strong degradation ability to PAEs, the degradation rate of DBP reaches 97.5%, the degradation rate of DEHP reaches 60.2%, and can use catechol, BA, PCA, PA, MBP, benzoic acid monobutyl ester, 4,5-dihydroxy phthalic acid, DEP, DMP and DOP and other various PAEs as substrate.The silkworm excrement is used as the carrier of fixed pseudomonas aeruginosa PS1, and the biological agent for repairing PAEs contaminated environment is prepared, is used to significantly improve soil pH, increase soil organic matter content and / or increase soil nitrate nitrogen and / or ammonium nitrogen content, increase the abundance of phylum Proteobacteria and / or phylum Synergistetes, and / or reduce the abundance of actinomycetales and / or Pseudonocardiales, so as to repair the environment contaminated by PAEs.

The invention provides a soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of resource utilization and biological energy sources. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 20 to 40 parts of coffee grounds, 10 to 30 parts of charcoal, 5 to 15 parts of bentonite, 3 to 10 parts of small molecular organic acid, 1 to 5 parts of metal ion chelating agent, 15 to 25 parts of organic fertilizer, 0.5 to 3 parts of microbial flora and 2 to 8 parts of humic acid; wherein the microbial bacteria are mixed bacteria and simultaneously comprise bacillus amyloliquefaciens, rhizosphere kocuria and salt-tolerant actinomycetes. According to the soil conditioner, the raw materials and the proportion of the conditioner are optimized, so that all the components have a synergistic effect, the improvement effect of the saline alkali soil is effectively improved, and the improvement process is accelerated.

The invention discloses an efficient liquid organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The liquid organic fertilizer is prepared from the following raw materials in parts by weight: 40 to 50 parts of livestock manure liquid material, 8 to 12 parts of compound microbial agent, 8 to 12 parts of seaweed extract, 6 to 10 parts of soybean meal hydrolysate, 10 to 15 parts of humic acid, 2 to 3 parts of medium trace element chelate, 0.5 to 1 part of chitosan oligosaccharide and 2 to 3 parts of brown sugar. In the compound microbial agent, the nutrient activation capability and the growth regulation function of acinetobacter tanshini and the disease prevention and control of actinoplanes rhizopus cooperate with each other, so that the requirement of cucumber growth on nutrients is met, powdery mildew is effectively prevented and controlled, meanwhile, the soil environment is improved, and the yield and the quality are both improved; the leavening agent is used for completing efficient decomposition conversion of the livestock manure; the two kinds of microbial agents promote each other and have synergistic interaction to form a complete action chain of decomposition-activation-synergism-disease prevention, and finally the application targets of cucumber growth promotion, yield improvement and quality optimization are achieved.

The invention belongs to the technical field of genetic engineering and environmental remediation, and particularly discloses construction of nitroreductase delivery plasmids and application of the nitroreductase delivery plasmids in environmental remediation. The nucleotide and amino acid sequences of the nitroreductase gene derived from the actinomycetes S16 functional metagenome are shown as SEQ ID NO. 1 and SEQ ID NO. 2. The method comprises the following steps: designing a primer by taking pQ-SacB-araC plasmid as a template, and carrying out PCR (Polymerase Chain Reaction); the method comprises the following steps: cloning an actinomycete S16 functional metagenome as a template, and designing a primer according to a target nitroreductase gene sequence to carry out PCR (Polymerase Chain Reaction) amplification; and purifying and recovering the two amplification products, and carrying out fragment recombination to obtain the nitroreductase delivery plasmid pQ-NR. The delivery plasmid pQ-NR and recombinant bacteria capable of stably and efficiently expressing nitroreductase are constructed, efficient delivery of nitroreductase genes in various microorganisms is achieved, and the application effect of the nitroreductase genes in actual environmental remediation related to nitro compound pollution is enhanced.

The invention relates to a plant soil disease control effect evaluation method, which is suitable for soil disease control effect evaluation of phytopathogen infection research, and realizes good growth of peanut root systems and controllability of pathogen infection intensity by optimizing a cultivation device, a matrix and an inoculation method. On the basis, 100 strains of actinomycetes are separated by taking faeces of protaetia brevitarsis larvae as a sample, strains with bacteriostatic activity on sclerotium rolfsii are screened through a plate confrontation experiment, and finally 7 strains of actinomycetes with remarkable antagonism are obtained. Through a soil disease control effect evaluation system, the growth promoting and disease resisting effects of the seven strains on peanuts are determined, and particularly, the X15 strain has the best performance. According to the method established by the research, actinomycetes with an excellent biocontrol function are effectively screened out, and important resources are provided for agricultural disease prevention and control and green biopesticide development.

The invention discloses a special alkaline organic-inorganic compound fertilizer for cucumbers and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The special alkaline organic-inorganic compound fertilizer for cucumbers comprises the following raw materials in parts by weight: 300-500 parts of an organic material, 28-36 parts of microbial powder, 250-350 parts of modified charcoal, 35-50 parts of a synergist, 100-200 parts of a nitrogen fertilizer, 50-100 parts of a phosphate fertilizer and 100-200 parts of a potassium fertilizer, wherein the microbial powder comprises actinoplanes dewengensis and trametes hirsutum. Actinoplanes dewardii can efficiently decompose organic materials, fully release nutrient substances and improve nutrient absorption of plants; the trametes hirsute can promote decomposition of organic materials and also has a growth promoting effect, and the trametes hirsute and the trametes hirsute have a synergistic effect, so that the quality and the yield of cucumbers are improved. The fertilizer effectively improves the physicochemical properties of soil, promotes the growth and development of cucumbers, improves the yield and quality of cucumbers, reduces the dependence on chemical fertilizers, reduces the fertilization cost, gives consideration to both quick-acting property and long-acting property, and reduces nutrient loss.

The application discloses a conditioner for sludge dewatering and synchronous fermentation and a preparation method thereof, and the conditioner is granular and sequentially comprises an inner core, an outer shell and a coating from inside to outside, wherein the mass fraction of the inner core is 1.5-2 parts, the inner core is pressed from coconut shell, and the density of the pressed coconut shell is 0.45-0.50 g / cm 3 ; the outer shell is composed of natural sand, corn shaft powder and a compound microbial agent, and the mass fractions of the three are 6-8 parts, 2-2.5 parts and 0.5-1 part respectively, wherein the natural sand has a fineness modulus of 2.5-3.0, and the mass fraction ratio of the inner core to the outer shell is 1:(5-6); the compound microbial agent is composed of streptococcus thermophilus and thermophilic actinomycetes, and the mass ratio of the two is 1:1; and the coating is composed of ethylene-vinyl acetate copolymer and polyvinyl alcohol, and the mass fractions of the two are 0.6-0.8 parts and 0.15-0.2 parts respectively. The conditioner can significantly shorten the sludge dewatering period and reduce the comprehensive treatment cost of the sludge.

This invention discloses a metabolomics-gut microbiome biomarker combination for the diagnosis of HIV-related asymptomatic neurocognitive impairment (ANI), comprising metabolomics biomarkers and gut microbiome biomarkers. The metabolomics biomarkers include plasma and fecal biomarkers. Plasma biomarkers include Asp-Asn and arachidonic acid, while fecal biomarkers include histidine, lysine, ornithine, and indolelactone. The gut microbiome biomarkers include phylum-level and genus-level biomarkers. Phylum-level biomarkers include Firmicutes, Actinobacteria, and Proteobacteria, while genus-level biomarkers include *Clostridium*, *Microbacterium*, *Klebsiella*, and *Prevotella*. This biomarker combination integrates the core differential features of metabolomics and gut microbiota, exhibiting high diagnostic specificity and sensitivity. It can effectively distinguish ANI patients from CI patients and healthy individuals, providing a clear target for HAND targeted intervention and possessing significant clinical application value.

The utility model relates to the technical field of biology, in particular to a pretreatment mechanism for cellulase actinomycete separation culture, which comprises a bottom plate, a support column and a mounting plate, a placing plate for placing a soil sample and a lifting component for changing the placing angle of the placing plate are arranged above the mounting plate, and the lifting component comprises a lifting component for lifting the soil sample to the placing angle of the placing plate. A motor bottom plate is fixedly mounted on the bottom face of the mounting plate, a rotatable lead screw is connected to the top end of the motor bottom plate, and the outer wall of the lead screw is sleeved with a lead screw sleeve. According to the air drying device, by arranging the lifting assembly, one side of the mounting frame can be lifted up through the lifting assembly in the using process of the device, so that the surface area in the containing plate can be exposed to the outside for air supply, the air drying speed is increased, and the overall working efficiency is improved.

The invention belongs to the technical field of microbial agriculture, and particularly relates to screening and application of indigenous actinomycetes for remarkably promoting disease resistance, yield increase and quality improvement of fritillaria taipaiensis, which is characterized by comprising the following specific steps: a, aseptically collecting samples from rhizosphere soil and endophytic tissues of fritillaria taipaiensis to ensure representativeness and no pollution of the samples; b, separating, purifying and identifying actinomycetes in the sample by utilizing a traditional microorganism separation and purification technology and combining a 16S rDNA sequencing technology, and constructing a comprehensive actinomycetes resource library; c, by constructing a multi-dimensional biological activity screening system, indigenous actinomycetes with remarkable activity promoting performance are screened and applied, and the prepared compound microbial agent can remarkably improve root development, photosynthesis efficiency and nutrient absorption capacity of the fritillaria taipaiensis. In a field test, the plant height, the leaf area and the root biomass of the fritillaria taipaiensis to which the microbial agent is applied are remarkably increased, so that the overall growth and development of the plant are promoted.