11 results about "Actinobacteria" patented technology

The invention relates to the technical field of soil improvement, and particularly discloses a soil conditioner based on microbial community regulation and control and a preparation method thereof, the soil conditioner comprises: (a) a composite functional flora comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium-dissolving actinomycetes and trichoderma, and the viable count ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the potassium-dissolving actinomycetes to the trichoderma is (1.0-1.5): (0.8-1.2): (0.5-1.0): (0.3-0.8); b) modified biochar carrier: the modified biochar carrier is prepared by oxygen-limited thermal cracking of agricultural wastes and is subjected to surface modification by a chitosan solution with a mass fraction of 3-5%; (c) a synergistic component which comprises humic acid particles and an enzymolysis seaweed extract; (d) a flora activator: compounding fructo-oligosaccharide and L-aspartic acid according to a mass ratio of 2: 1; through the synergistic effect of multifunctional composite flora of nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing actinomycetes and trichoderma, the biotransformation process of nitrogen, phosphorus and potassium elements in soil is synchronously activated, and the modified charcoal carrier is subjected to multi-stage pyrolysis and chitosan modification to form a graded pore structure.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The invention discloses a method for preparing an amino acid-containing water-soluble fertilizer from a fox racoon dog carcass. The method comprises the following steps: cutting and crushing the fox racoon dog carcass to obtain meat paste; mixing the meat paste with a microbial agent according to a material-liquid ratio of 1.8: 1; the microbial agent comprises thermoactinomycetes, bacillus subtilis and bacillus licheniformis which are mixed according to the volume ratio of 1: 1.3: 2, an enzyme mixture is added into the meat paste according to the mass ratio of 4: 1, an oxygen producer is added into the meat paste until the meat paste cannot be seen by naked eyes, the reaction is ended, oil-water separation is conducted after filtering, and an oil-water separation product is obtained; and performing standard regulation on the deoiled liquid to obtain the amino acid-containing water-soluble fertilizer. According to the method, thorough decomposition of the carcasses is achieved through the compatible microbial agents, the filtered sediment can be decomposed repeatedly, no solid waste is generated, environmental pollution is avoided, and a safe and traceable recycling way is provided for the fox raccoon dog carcasses.

This invention discloses an S-type α-fluoroimine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from actinomycetes. Actinomadura welshii By using directed evolution to modify its active site, S-type α-fluoroimine reductase mutants with high activity, stereoselectivity, and high yield of 4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline were obtained. These mutants were D98G, D98G / M122L, or D98G / M122L / W216V. This invention achieves efficient synthesis of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline from inexpensive (trans)-2,2,2-trifluoro-N-(4-methoxyphenyl)-1-phenylethylimine, which is beneficial for the industrial synthesis and application of (S)-4-methoxy-N-(2,2,2-trifluoro-1-phenylethyl)aniline.

The invention provides a soil conditioner as well as a preparation method and application thereof, and belongs to the technical field of resource utilization and biological energy sources. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 20 to 40 parts of coffee grounds, 10 to 30 parts of charcoal, 5 to 15 parts of bentonite, 3 to 10 parts of small molecular organic acid, 1 to 5 parts of metal ion chelating agent, 15 to 25 parts of organic fertilizer, 0.5 to 3 parts of microbial flora and 2 to 8 parts of humic acid; wherein the microbial bacteria are mixed bacteria and simultaneously comprise bacillus amyloliquefaciens, rhizosphere kocuria and salt-tolerant actinomycetes. According to the soil conditioner, the raw materials and the proportion of the conditioner are optimized, so that all the components have a synergistic effect, the improvement effect of the saline alkali soil is effectively improved, and the improvement process is accelerated.

The invention discloses an efficient liquid organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The liquid organic fertilizer is prepared from the following raw materials in parts by weight: 40 to 50 parts of livestock manure liquid material, 8 to 12 parts of compound microbial agent, 8 to 12 parts of seaweed extract, 6 to 10 parts of soybean meal hydrolysate, 10 to 15 parts of humic acid, 2 to 3 parts of medium trace element chelate, 0.5 to 1 part of chitosan oligosaccharide and 2 to 3 parts of brown sugar. In the compound microbial agent, the nutrient activation capability and the growth regulation function of acinetobacter tanshini and the disease prevention and control of actinoplanes rhizopus cooperate with each other, so that the requirement of cucumber growth on nutrients is met, powdery mildew is effectively prevented and controlled, meanwhile, the soil environment is improved, and the yield and the quality are both improved; the leavening agent is used for completing efficient decomposition conversion of the livestock manure; the two kinds of microbial agents promote each other and have synergistic interaction to form a complete action chain of decomposition-activation-synergism-disease prevention, and finally the application targets of cucumber growth promotion, yield improvement and quality optimization are achieved.

The invention discloses a special alkaline organic-inorganic compound fertilizer for cucumbers and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The special alkaline organic-inorganic compound fertilizer for cucumbers comprises the following raw materials in parts by weight: 300-500 parts of an organic material, 28-36 parts of microbial powder, 250-350 parts of modified charcoal, 35-50 parts of a synergist, 100-200 parts of a nitrogen fertilizer, 50-100 parts of a phosphate fertilizer and 100-200 parts of a potassium fertilizer, wherein the microbial powder comprises actinoplanes dewengensis and trametes hirsutum. Actinoplanes dewardii can efficiently decompose organic materials, fully release nutrient substances and improve nutrient absorption of plants; the trametes hirsute can promote decomposition of organic materials and also has a growth promoting effect, and the trametes hirsute and the trametes hirsute have a synergistic effect, so that the quality and the yield of cucumbers are improved. The fertilizer effectively improves the physicochemical properties of soil, promotes the growth and development of cucumbers, improves the yield and quality of cucumbers, reduces the dependence on chemical fertilizers, reduces the fertilization cost, gives consideration to both quick-acting property and long-acting property, and reduces nutrient loss.

This invention discloses a metabolomics-gut microbiome biomarker combination for the diagnosis of HIV-related asymptomatic neurocognitive impairment (ANI), comprising metabolomics biomarkers and gut microbiome biomarkers. The metabolomics biomarkers include plasma and fecal biomarkers. Plasma biomarkers include Asp-Asn and arachidonic acid, while fecal biomarkers include histidine, lysine, ornithine, and indolelactone. The gut microbiome biomarkers include phylum-level and genus-level biomarkers. Phylum-level biomarkers include Firmicutes, Actinobacteria, and Proteobacteria, while genus-level biomarkers include *Clostridium*, *Microbacterium*, *Klebsiella*, and *Prevotella*. This biomarker combination integrates the core differential features of metabolomics and gut microbiota, exhibiting high diagnostic specificity and sensitivity. It can effectively distinguish ANI patients from CI patients and healthy individuals, providing a clear target for HAND targeted intervention and possessing significant clinical application value.

The invention belongs to the technical field of microbial agriculture, and particularly relates to screening and application of indigenous actinomycetes for remarkably promoting disease resistance, yield increase and quality improvement of fritillaria taipaiensis, which is characterized by comprising the following specific steps: a, aseptically collecting samples from rhizosphere soil and endophytic tissues of fritillaria taipaiensis to ensure representativeness and no pollution of the samples; b, separating, purifying and identifying actinomycetes in the sample by utilizing a traditional microorganism separation and purification technology and combining a 16S rDNA sequencing technology, and constructing a comprehensive actinomycetes resource library; c, by constructing a multi-dimensional biological activity screening system, indigenous actinomycetes with remarkable activity promoting performance are screened and applied, and the prepared compound microbial agent can remarkably improve root development, photosynthesis efficiency and nutrient absorption capacity of the fritillaria taipaiensis. In a field test, the plant height, the leaf area and the root biomass of the fritillaria taipaiensis to which the microbial agent is applied are remarkably increased, so that the overall growth and development of the plant are promoted.