Method for preparing amino acid-containing water-soluble fertilizer from fox racoon dog carcasses

Abstract

The invention discloses a method for preparing an amino acid-containing water-soluble fertilizer from a fox racoon dog carcass. The method comprises the following steps: cutting and crushing the fox racoon dog carcass to obtain meat paste; mixing the meat paste with a microbial agent according to a material-liquid ratio of 1.8: 1; the microbial agent comprises thermoactinomycetes, bacillus subtilis and bacillus licheniformis which are mixed according to the volume ratio of 1: 1.3: 2, an enzyme mixture is added into the meat paste according to the mass ratio of 4: 1, an oxygen producer is added into the meat paste until the meat paste cannot be seen by naked eyes, the reaction is ended, oil-water separation is conducted after filtering, and an oil-water separation product is obtained; and performing standard regulation on the deoiled liquid to obtain the amino acid-containing water-soluble fertilizer. According to the method, thorough decomposition of the carcasses is achieved through the compatible microbial agents, the filtered sediment can be decomposed repeatedly, no solid waste is generated, environmental pollution is avoided, and a safe and traceable recycling way is provided for the fox raccoon dog carcasses.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of water-soluble fertilizer, and particularly relates to a method for preparing water-soluble fertilizer containing amino acids from fox body. BACKGROUND

[0002] Although the amount of fox skin taken has decreased in recent years, the problem of body processing is still prominent due to the large-scale breeding, and the existing processing system is imperfect and untraceable, which has a safety hazard of flowing into the food market, and resource recycling has become a key problem to be solved in the characteristic breeding industry.

[0003] At present, the production raw materials of water-soluble fertilizer containing amino acids are mainly livestock and poultry protein waste (such as leather, hair, and dead livestock), and the protein decomposition methods mainly include physical, chemical, enzyme and microbial decomposition. Physical and chemical decomposition requires extreme conditions such as high temperature and high pressure or strong acid and strong base, which easily destroys the cyclic amino acid structure and has high requirements for equipment; while enzyme and microbial decomposition has mild reaction conditions, can preserve the amino acid structure, and the product is pollution-free to the environment, which has more advantages. However, in the prior art, there is no report on the technology of preparing water-soluble fertilizer containing amino acids in accordance with the national standard by using fox body through enzyme and microbial decomposition. In addition, the local standard of Qinhuangdao City in 2023, "Technical Regulations for Producing Amino Acid and Polypeptide Solution by Using Fox Body", has defects such as complex process (including steps such as leaching and antibiotic removal), limited raw materials to carcass (without internal organs), and unclear residue treatment, which is difficult to meet the efficient and environmentally friendly resource utilization demand. Therefore, a method for preparing water-soluble fertilizer containing amino acids from fox body is needed. SUMMARY

[0004] The purpose of the present application is to provide a method for preparing water-soluble fertilizer containing amino acids from fox body to solve the problems existing in the prior art.

[0005] To achieve the above purpose, the present application is implemented according to the following technical solutions:

[0006] On the one hand, the present application includes the following steps:

[0007] The fox body is cut and stirred to obtain meat paste; the meat paste is mixed with a bacterial agent at a material-liquid ratio of 1.8:1; the bacterial agent includes high-temperature actinomycetes, Bacillus subtilis and Bacillus licheniformis mixed at a volume ratio of 1:1.3:2, an enzyme mixture is added to the meat paste at a mass ratio of 4:1, neutral protease, alkaline protease and acid protease are added to the enzyme mixture at a mass ratio of 1:1.25:1.5, an oxygen enhancer is added to the meat paste, and then the reaction is carried out at 50-60 DEG C for 12-15 hours, stirring once every 1 hour, until the meat paste is not visible to the naked eye, and then the reaction is stopped; after filtration, oil-water separation is carried out, and the liquid after oil removal is adjusted to obtain water-soluble fertilizer containing amino acids.

[0008] Further, the bacillus licheniformis is received by China Typical Culture Collection Center on May 26, 2025, and the preservation number is CCTCC NO: M20251187, the name is bacillus licheniformis HBTS240717, Latin: Bacillus licheniformis HBTS240717, and the preservation address is No. 299, Bayi Road, Luojia Hill Street, Wuchang District, Wuhan City, Hubei Province.

[0009] Further, food-grade magnesium sulfate is added in the standard regulation at 30-50 g / L, and stirring and dissolving are carried out; and the total amount of medium elements is ensured to be greater than 30 g / L.

[0010] Further, the oxygenation agent is sodium percarbonate or calcium percarbonate, and the addition amount is 0.5-1% of the weight of the meat paste.

[0011] Further, the obtained precipitate is added into the bacterial agent, enzyme mixture and oxygenation agent again for decomposition.

[0012] The beneficial effects of the present application are:

[0013] The present application is a method for preparing amino acid-containing water-soluble fertilizer from fox body, which has the following technical effects compared with the prior art:

[0014] The present application realizes complete decomposition of the body by the compatible bacterial agent, and the filtered precipitate can be repeatedly decomposed, so that no solid waste is generated, environmental pollution is avoided, and a safe and traceable resource utilization way for the fox body is provided. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a production process flowchart of the method for preparing amino acid-containing water-soluble fertilizer from fox body of the present application;

[0016] Figure 2 It is a comparison chart of the influence of the amino acid-containing water-soluble fertilizer of the method for preparing amino acid-containing water-soluble fertilizer from fox body of the present application on the yield of winter wheat round selection 149;

[0017] Figure 3 It is a comparison chart of the influence of the amino acid-containing water-soluble fertilizer of the method for preparing amino acid-containing water-soluble fertilizer from fox body of the present application on the number of peanut flower needles;

[0018] Figure 4 It is a comparison chart of the influence of the amino acid-containing water-soluble fertilizer of the method for preparing amino acid-containing water-soluble fertilizer from fox body of the present application on the number of peanut results; DETAILED DESCRIPTION

[0019] The application will be further described in the following specific examples, the illustrative examples of the application and the description used to explain the application, but not as a limitation of the application.

[0020] As Figure 1 shown, the application is a method for preparing amino acid-containing water-soluble fertilizer from fox carcasses, comprising the following steps:

[0021] Step 1: Raw material preparation: purchase fox carcasses and remove bones;

[0022] Step 2: Grinding: grind the fox carcass residues;

[0023] Step 3: Decomposition: add the meat paste and bacterial agents at a ratio of 1.8:1, the bacterial agents include high-temperature actinomycetes, Bacillus subtilis, and Bacillus licheniformis, the volume ratio of the three bacterial agents is 1:1.3:2, and the concentration is 10 7 ~ 10 8 CFU / mL; add neutral protease, alkaline protease, and acid protease at a ratio of 4:1 according to the amount of meat paste and total enzyme (mass ratio 1:1.25:1.5); at the same time, add the oxygen enhancer sodium percarbonate or calcium percarbonate in an amount of 0.5-1% of the weight of the meat paste. React at 50-60℃ for 12-15h, stir every 1h, and end the reaction when the meat paste is not visible to the naked eye;

[0024] Step 4: Filtration: filter with a 300-mesh stainless steel filter screen;

[0025] Step 5: Oil-water separation: high temperature 95℃, static stratification, and absorption of the upper layer of oil to obtain biological oil. The liquid after oil removal is the amino acid-containing water-soluble fertilizer;

[0026] Step 6: Add an appropriate amount of magnesium sulfate according to the volume of the amino acid-containing water-soluble fertilizer to meet the requirements of the amino acid-containing water-soluble fertilizer standard (medium element) NY1429-2010;

[0027] Step 7: Precipitation: add the three bacterial agents, enzyme amount, and oxygen enhancer according to the above ratio for re-decomposition.

[0028] In this embodiment, the fox body is skinned, the bones are removed, the internal organs are retained, the meat is cut and stirred to obtain meat paste, the meat paste is mixed with bacterial agents at a solid-liquid ratio of 1.8:1, the bacterial agents include high-temperature actinomycetes (frozen powder + dissolving solution of SHMCC ordinary high-temperature actinomycetes SHBCC D12698 specification type from Shanghai Microorganism Center), Bacillus subtilis (frozen powder + dissolving solution of SHMCC Bacillus subtilis SHBCC D11186 specification type from Shanghai Microorganism Center) and Bacillus licheniformis (received by China Typical Culture Collection Center on May 26, 2025, the preservation number is CCTCC NO: M20251187, the name is Bacillus licheniformis HBTS240717, Latin: Bacillus licheniformis HBTS240717), and the volume ratio of the three bacterial agents is 1:1.3:2, and the concentration is 10 7 ~ 10 8 CFU / mL; neutral protease, alkaline protease and acid protease are added at a ratio of 4:1 according to the meat paste and the total enzyme amount (mass ratio 1:1.25:1.5); at the same time, the amount of oxygen enhancer sodium percarbonate or calcium percarbonate is 0.5-1% of the weight of the meat paste. 50-60℃ reaction for 12-15h, stirring every 1h. When the meat paste is not visible to the naked eye, the reaction is stopped, filtered with a 300 mesh stainless steel filter screen, the filtrate is high temperature 95℃, and the upper layer oil is obtained by standing and sucking. Biological oil. The filtrate after oil removal is an amino acid water-soluble fertilizer containing 110g / L of amino acid; the precipitate is re-decomposed by adding three bacterial agents, enzyme amount and oxygen enhancer according to the above proportion, the concentration is 10 8 CFU / mL, 0.75% of the weight of the meat paste, 55℃ reaction for 15h.

[0029] 2024-2025, in Hebei Science and Technology Normal College, Shigezhuang Farm, Qinhuangdao City, Hebei Province, winter wheat round selection 149 was used as test material, the base fertilizer was 6kg of pure nitrogen (N6) compound fertilizer per mu, a split-plot experiment design was adopted, the main area was set at the jointing stage, 5kg of nitrogen fertilizer level per mu was set: no nitrogen (NO), 6kg per mu (N6), 8kg per mu (N8), 8kg per mu (N10), and 8kg per mu (N12); the sub-area was set to contain amino acid water-soluble fertilizer (AA) concentration 0g / L, 0.4g / L and 0.8g / L, and was sprayed at the heading stage, flowering stage and 7d after flowering, a total of 3 times.

[0030] Test results

[0031] Effect of spraying amino acid water-soluble fertilizer on winter wheat yield

[0032] At the jointing stage, low nitrogen application levels (N0 and N6) all showed increased yields with increasing AA concentration. For nitrogen application levels N8–N12, the highest yield was achieved with a 0.4 g / L AA concentration. Under the N6 treatment, the yield with a 0.8 g / L AA concentration was 10.7% higher than the no-fertilizer treatment; under the N8 treatment, the yield with a 0.4 g / L AA concentration was 5.7% higher than the no-fertilizer treatment. Meanwhile, the yields of the N6 treatment with a 0.8 g / L AA concentration and the N8 treatment with a 0.4 g / L AA concentration were 484.5 kg / mu and 491.5 kg / mu, respectively, with no significant difference between the two treatments. Figure 2 As shown. Therefore, compared with the total nitrogen of 16 kg / mu (10 kg / mu topdressing at the jointing stage, N10) for winter wheat variety Lunxuan 149, the nitrogen reduction per mu was 2 kg, i.e., a nitrogen reduction of 12.5% ​​(8 kg / mu topdressing at the jointing stage, N8) and 4 kg / mu, i.e., a nitrogen reduction of 25% (6 kg / mu topdressing at the jointing stage, N6). Moreover, there was no significant difference in yield between the two treatments when spraying 0.4 g / L and 0.8 g / L AAA, respectively, indicating that spraying amino acid-containing water-soluble fertilizer can increase yield under a certain nitrogen application rate.

[0033] From May 1, 2025 to July 25, 2025, a two-factor experiment was conducted in Zhengzhuang Village, Youzha Town, Luanzhou City, Tangshan City, Hebei Province, using two peanut varieties, Kainong 1760 and Huayu 23, as test materials. Five concentrations of water-soluble fertilizer containing amino acids (0 g / L, 0.4 g / L, 0.6 g / L, 0.8 g / L, and 1.0 g / L) were sprayed at the seedling, flowering, and pod-setting stages. On July 25, samples were taken to determine the number of flower pebbles (excluding those with fruit) and the number of pods.

[0034] Test results

[0035] The effect of spraying amino acid-containing water-soluble fertilizer on the number of peanut pedicles

[0036] like Figure 3 As shown, within the AA concentration range of 0–0.6 g / L, the number of peculiarities in both peanut varieties increased with increasing AA concentration, and was significantly higher than in the untreated group. High concentrations of AA significantly reduced the number of peculiarities: Huayu 23 treated with 0.8 g / L AA showed a reduction in peculiarities, but the difference was not significant compared to 0.6 g / L AA; Kainong 1760 treated with 0.8 g / L AA showed a significantly higher number of peculiarities than other treatments. Therefore, AA application can increase the number of peculiarities, but the two varieties have different sensitivities to AA, with Kainong 1760 being more sensitive.

[0037] Effects of spraying amino acid-containing water-soluble fertilizer on peanut pod number

[0038] In the range of 0-0.6 g / L AA, the number of fruits of the two peanut varieties increased with the increase of AA concentration, and were significantly higher than that of the treatment without AA spraying. The reaction of different varieties was different when high concentration AA was sprayed. The number of fruits of Huayu 23 sprayed with 0.8 g / L AA decreased, but had no significant difference with other treatments (except 0 g / L). The number of fruits of Kaikang 1760 sprayed with 0.8 g / L AA was significantly higher than that of 1.0 g / L AA, and had no significant difference with that of 0.6 g / L AA. Figure 4 Therefore, spraying AA can increase the number of fruits, and the sensitivity of the two varieties to AA is different, and Kaikang 1760 is more sensitive.

[0039] In the above Figures 2-4 In the above, a, b, c, d, e, f, g, h, i, j, k different lowercase letters represent that the difference between different treatments reaches a significant level (p<0.05).

[0040] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method for preparing an amino acid-containing water-soluble fertilizer from a fox carcass, characterized by, The method comprises the following steps: Cutting and chopping a fox body to obtain meat paste; Mixing the meat paste with a bacterial agent at a material-liquid ratio of 1.8:1; the bacterial agent comprises high-temperature actinomycetes, Bacillus subtilis and Bacillus licheniformis mixed at a volume ratio of 1:1.3:2, an enzyme mixture is added to the meat paste at a mass ratio of 4:1, neutral protease, alkaline protease and acid protease are added to the enzyme mixture at a mass ratio of 1:1.25:1.5, an oxygenation agent is added to the meat paste, and then the mixture is reacted at 50-60 DEG C for 12-15 hours, with stirring every 1 hour, until the meat paste is invisible to the naked eye; after filtration, oil-water separation is performed, and the liquid after oil removal is subjected to standard adjustment to obtain an amino acid water-soluble fertilizer.

2. The method of preparing amino acid-containing water-soluble fertilizer from a fox carcass according to claim 1, characterized by, The Bacillus licheniformis was received by the China Center for Type Culture Collection on May 26, 2025, and was preserved with a preservation number of CCTCC NO:M20251187, a name of Bacillus licheniformis HBTS240717, a Latin name of Bacillus licheniformis HBTS240717, and an address of 299 Bao Yi Road, Laojia Hill Street, Wuchang District, Wuhan City, Hubei Province.

3. The method of preparing amino acid-containing water-soluble fertilizer from a fox carcass according to claim 1, characterized by, In the standard adjustment, food-grade magnesium sulfate is added at 30-50 g / L, and is stirred and dissolved to ensure that the total amount of medium elements is greater than or equal to 30 g / L.

4. The method of preparing amino acid-containing water-soluble fertilizer from a fox body according to claim 1, characterized by, The oxygenation agent is sodium percarbonate or calcium percarbonate, and the addition amount is 0.5-1% of the weight of the meat paste.

5. The method of preparing amino acid-containing water-soluble fertilizer from a fox body according to claim 1, characterized by, The obtained precipitate is re-added to the bacterial agent, the enzyme mixture and the oxygenation agent for re-decomposition. The method comprises the following steps: Cutting and chopping a fox body to obtain meat paste; Mixing the meat paste with a bacterial agent at a material-liquid ratio of 1.8:1; the bacterial agent comprises high-temperature actinomycetes, Bacillus subtilis and Bacillus licheniformis mixed at a volume ratio of 1:1.3:2, an enzyme mixture is added to the meat paste at a mass ratio of 4:1, neutral protease, alkaline protease and acid protease are added to the enzyme mixture at a mass ratio of 1:1.25:1.5, an oxygenation agent is added to the meat paste, and then the mixture is reacted at 50-60 DEG C for 12-15 hours, with stirring every 1 hour, until the meat paste is invisible to the naked eye; after filtration, oil-water separation is performed, and the liquid after oil removal is subjected to standard adjustment to obtain an amino acid water-soluble fertilizer. The Bacillus licheniformis was received by the China Center for Type Culture Collection on May 26, 2025, and was preserved with a preservation number of CCTCC NO:M20251187, a name of Bacillus licheniformis HBTS240717, a Latin name of Bacillus licheniformis HBTS240717, and an address of 299 Bao Yi Road, Laojia Hill Street, Wuchang District, Wuhan City, Hubei Province. In the standard adjustment, food-grade magnesium sulfate is added at 30-50 g / L, and is stirred and dissolved to ensure that the total amount of medium elements is greater than or equal to 30 g / L. The oxygenation agent is sodium percarbonate or calcium percarbonate, and the addition amount is 0.5-1% of the weight of the meat paste. The obtained precipitate is re-added to the bacterial agent, the enzyme mixture and the oxygenation agent for re-decomposition.

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