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Root nodule growth-promoting microbacterium X-18 and application thereof

A technology of X-18 and microbacteria, applied in the field of microorganisms, to achieve the effect of symbiotic nitrogen fixation of nodule-promoting bacteria and plants, promising development prospects, and promoting effective nitrogen fixation

Active Publication Date: 2019-12-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on screening suitable root nodule growth-promoting strains for different plants

Method used

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  • Root nodule growth-promoting microbacterium X-18 and application thereof
  • Root nodule growth-promoting microbacterium X-18 and application thereof
  • Root nodule growth-promoting microbacterium X-18 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] The acquisition and identification of embodiment 1 bacterial strain

[0027] Soil samples were collected from the rhizosphere soil 5 cm below the surface of the slopes on both sides of Yueyang Avenue, Yueyang City, and the diluted coating plate method was used to prepare nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder) ; Sodium chloride 5g; Agar 15g; Sterile water 1000mL) cultured for 2-3 days, picked different colonies by visual observation, and repeatedly streaked and purified to obtain different single colonies.

[0028] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Microbacterium chocolatem was 99.75%. Combined with morphological characteristics and 16S rDNA g...

Embodiment 2

[0030] The ability of embodiment 2 bacterial strains to promote black locust nodulation and nitrogen fixation

[0031] 1. Preparation of fermentation broth: 1) Take Microbacterium X-18 from the slant, activate it on nutrient agar solid medium at 35°C for 24 hours; Into 20mL LB liquid medium, 35°C, 200r / min constant temperature shaking for 24h to prepare the seed liquid; 3) Take the seed liquid according to the inoculum amount of 3% to inoculate the liquid medium, 35°C, 200r / min shaker and cultivate to OD 560 0.8-1.2 to obtain the fermented bacterial liquid; 4) before use, dilute the fermented bacterial liquid obtained in step 3) by 100 times with sterile water, and directly spray it in the planted black locust seedling pot.

[0032] 2. Potted plant experiment: according to 60mL / pot, take the diluted fermented bacteria liquid and add it around the planted Robinia pseudoacacia seedlings (the actual planting period is 4 months, the first month is the thinning stage, no bacteria, ...

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Abstract

The invention discloses a root nodule growth-promoting microbacterium X-18 and application thereof and belongs to the technical field of microorganisms. A root nodule growth-promoting bacterium is classified and named as microbacterium sp. X-18, is deposited in The preservation date is preserved in China Center for Type Culture Collection with a preservation date being April 8th, 2019, a preservation number being CCTCC NO: M2019236 and a preservation address being Wuhan University in China. The microbacterium X-18 disclosed by the invention is applied to black locust as leguminous plants, canpromote effective nitrogen fixation of the black locust and provides nitrogen required by growth of he black locust to enable the symbiotic fixation ability of the root nodule growth-promoting bacteria with the plants to be obviously exerted, so that growth and development of black locust plants are promoted, and a development prospect is good.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a nodule-promoting bacterium Microbacterium sp. X-18 and an application thereof. Background technique [0002] Soil spraying technology is a greening technology that sprays the mixture of soil growth matrix materials and plant seeds evenly and at high pressure on rock and soil slopes. The growth matrix material mixture of this technology is prepared with screened soil bacteria, which is mainly a greening technology developed for hard slopes such as rocks and for plants to create a suitable growth environment on hard slopes. As one of the important components of the growth matrix material of this technology, the screened soil bacteria play two roles: one is to respond to the change of soil ecological mechanism and environmental stress, accelerate the erosion of rock mass, and effectively improve the Substrate interface fusion; the second is to promote the growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
CPCC12N1/205C12R2001/01
Inventor 庄家尧刘超王阳阳
Owner NANJING FORESTRY UNIV
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