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A kind of nodule-promoting bacterium Microbacterium x-18 and its application

A technology of X-18 and microbacteria is applied in the field of microorganisms to achieve the effect of promoting effective nitrogen fixation, symbiotic nitrogen fixation of root nodule growth-promoting bacteria and plants, and promising development prospects.

Active Publication Date: 2020-11-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few domestic reports on screening suitable root nodule growth-promoting strains for different plants

Method used

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  • A kind of nodule-promoting bacterium Microbacterium x-18 and its application
  • A kind of nodule-promoting bacterium Microbacterium x-18 and its application
  • A kind of nodule-promoting bacterium Microbacterium x-18 and its application

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Experimental program
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Effect test

Embodiment 1

[0026] The acquisition and identification of embodiment 1 bacterial strain

[0027] Soil samples were collected from the rhizosphere soil 5 cm below the surface of the slopes on both sides of Yueyang Avenue, Yueyang City, and the diluted coating plate method was used to prepare nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder) ; Sodium chloride 5g; Agar 15g; Sterile water 1000mL) cultured for 2-3 days, picked different colonies by visual observation, and repeatedly streaked and purified to obtain different single colonies.

[0028] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Microbacterium chocolatem was 99.75%. Combined with morphological characteristics and 16S rDNA g...

Embodiment 2

[0030] The ability of embodiment 2 bacterial strains to promote black locust nodulation and nitrogen fixation

[0031] 1. Preparation of fermentation broth: 1) Take Microbacterium X-18 from the slant, activate it on nutrient agar solid medium at 35°C for 24 hours; Into 20mL LB liquid medium, 35°C, 200r / min constant temperature shaking for 24h to prepare the seed liquid; 3) Take the seed liquid according to the inoculum amount of 3% to inoculate the liquid medium, 35°C, 200r / min shaker and cultivate to OD 560 0.8-1.2 to obtain the fermented bacterial liquid; 4) before use, dilute the fermented bacterial liquid obtained in step 3) by 100 times with sterile water, and directly spray it in the planted black locust seedling pot.

[0032] 2. Potted plant experiment: according to 60mL / pot, take the diluted fermented bacteria liquid and add it around the planted Robinia pseudoacacia seedlings (the actual planting period is 4 months, the first month is the thinning stage, no bacteria, ...

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Abstract

The invention discloses a nodule-promoting bacterium Microbacterium genus X‑18 and an application thereof, belonging to the technical field of microorganisms. The classification of the nodule-promoting bacterium is named Microbacterium sp. X-18, and it is preserved in the China Type Culture Collection Center, with a preservation date of: April 8, 2019, a preservation number CCTCC No: M2019236, and a preservation address : Wuhan University, Wuhan, China. Microbacterium X‑18 of the present invention is applied to black locust of leguminous plant, can promote the effective nitrogen fixation of black locust, provide the nitrogen needed for the growth of black locust, make the symbiotic nitrogen fixation ability of nodule-promoting bacteria and plants be obviously brought into play, thereby promote the growth of black locust plants growth and development, the development prospects are promising.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a nodule-promoting bacterium Microbacterium sp. X-18 and an application thereof. Background technique [0002] Soil spraying technology is a greening technology that sprays the mixture of soil growth matrix materials and plant seeds evenly and at high pressure on rock and soil slopes. The growth matrix material mixture of this technology is prepared with screened soil bacteria, which is mainly a greening technology developed for hard slopes such as rocks and for plants to create a suitable growth environment on hard slopes. As one of the important components of the growth matrix material of this technology, the screened soil bacteria play two roles: one is to respond to the change of soil ecological mechanism and environmental stress, accelerate the erosion of rock mass, and effectively improve the Substrate interface fusion; the second is to promote the growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/205C12R2001/01
Inventor 庄家尧刘超王阳阳
Owner NANJING FORESTRY UNIV
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