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Microbacterium capable of degrading decabromodiphenyl ether as well as domestication method and application thereof

A decabromodiphenyl ether and microbacteria technology, which is applied in chemical instruments and methods, microorganism-based methods, and methods using microorganisms, etc., can solve the problems of lack of effective degrading bacteria, low efficiency, etc., and achieves low cost and degradation. Good effect and strong environmental adaptability

Active Publication Date: 2019-11-29
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial degradation of decabromodiphenyl ether is often not efficient in the actual application process, and the microbial degradation of decabromodiphenyl ether is limited due to the lack of effective degradation strains. Therefore, the screening and application of decabromodiphenyl ether efficient degrading bacteria certain research significance

Method used

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  • Microbacterium capable of degrading decabromodiphenyl ether as well as domestication method and application thereof
  • Microbacterium capable of degrading decabromodiphenyl ether as well as domestication method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Domestication and screening of degrading strains

[0046] (1) Add the decabromodiphenyl ether-contaminated soil to 20 mL of inorganic salt medium (sterilized), and cultivate it in a constant temperature shaker with a temperature of 30 ° C and a rotation speed of 160 rpm for 4 hours, and then take it out. Stand still to obtain a supernatant, which is used as an inoculum to continue acclimatization. Each liter of inorganic salt medium includes: 1g / L (NH4) 2 SO 4 , 1.5g / L KH 2 PO 4 , 3g / LK 2 HPO 4 , 2mL / L trace element solution, 1000mL distilled water, pH 7.0.

[0047] The trace element solution is a solution obtained by adding trace element compounds into water and mixing them evenly; in the trace element solution, the concentration of each substance is: MgSO 4 The concentration is 4.2g / L, ZnSO 4 The concentration is 4.2g / L, CuSO 4 The concentration is 1.2g / L, MnSO 4 The concentration is 1.2g / L, FeSO 4 ·7H 2 The concentration of O is 1.2g / L and CaCl 2 The ...

Embodiment 2

[0056] Example 2 Degradation Analysis of Microbacteria to Decabromodiphenyl Ether (Effect Verification)

[0057] Microbacterium sp was inoculated into a 1000mL Erlenmeyer flask containing 500mL of sterilized enrichment medium, placed in a constant temperature shaker at a temperature of 30°C and a rotation speed of 160rpm, and cultured for 36 hours to obtain enriched cultured bacteria. Liquid; the enrichment medium (in terms of one liter volume) contains: 3g / L beef extract, 10g / L peptone, 5g / L NaCl, 1000mL distilled water, pH 7.0.

[0058] Place the enriched cultured bacterial solution (20mL) in a 50mL sterilized centrifuge tube, centrifuge at 8000g for 7min, collect the bacteria, wash repeatedly with sterile normal saline and centrifuge for 3 times, and finally add normal saline to make a certain concentration (OD 600 =0.6) of the bacterial suspension, 1mL of the configured bacterial suspension was added to 19mL of sterilized degradation medium (decabromodiphenyl ether degrad...

Embodiment 3

[0070]Example 3 is the same as Example 2, except that in the degradation experiment, the pH of the degradation medium is 7.0, and the culture conditions are as follows: the temperature is 20°C, 25°C, 30°C, 35°C and 40°C, the rotating speed of the shaker is 160rpm, and the culture time for 7d. Finally, the degradation rates of decabromodiphenyl ether by microbacteria were 27.5%, 40.6%, 57.6%, 55.1% and 49.8%. The degradation effect of embodiment 3 is similar to embodiment 2, can refer to figure 2 .

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Abstract

The invention discloses a microbacterium capable of degrading decabromodiphenyl ether as well as a domestication method and an application thereof. The microbacterium sp capable of degrading decabromodiphenyl ether is preserved in the Guangdong Province Microbial Culture Collection Center on April 19th, 2019, and a number of the microbacterium sp is GDMCC No: 60634. The domestication method is a method for increasing pollutants in a gradient mode, decabromodiphenyl ether is added into a culture medium, the concentration gradient of the decabromodiphenyl ether ranges from 10 mg / L to 100 mg / L, and domestication is conducted through the domestication method for increasing the concentration of the pollutants in the gradient mode. The microbacterium provided by the invention is high in environmental adaptability and relatively good in decabromodiphenyl ether degradation effect; and the cost of degrading pollutants by using the microbacterium is relatively low. The degradation rate of the microbacterium provided by the invention on decabromodiphenyl ether can reach 57.6% at most, the practical application value is high, and a reference can be provided for solving the problem of decabromodiphenyl ether pollution treatment.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a microbacterium capable of degrading decabromodiphenyl ether and its domestication method and application. Background technique [0002] Decabromodiphenyl ether (BDE-209) is a common brominated flame retardant, which is widely used in textiles, furniture, electronics and interior decoration due to its low price, high flame retardancy and other advantages. At present, due to long-term and large-scale use, a large amount of decabromodiphenyl ether has directly or indirectly entered into environmental media such as air, dust, water, sediment, soil, organisms, etc., and even human body has detected decabromodiphenyl ether. There is evidence that decabromodiphenyl ether in the environment can enter the human body through breathing, eating, accidental ingestion, etc. It interferes with the secretion of thyroid hormones, has ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34B09C1/10C12R1/01C02F101/36
CPCC12N1/20C12N1/36C02F3/34B09C1/10C02F2101/36C12R2001/01C12N1/205
Inventor 尹华余元元刘影
Owner SOUTH CHINA UNIV OF TECH
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