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Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters

A microbacterium, J-1 technology, applied in the field of biological treatment of environmental pollutants, to achieve the effect of strong adaptability, wide distribution, and wide application prospects

Active Publication Date: 2015-07-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to provide microbacteria ( Microbacterium sp.) Application of J-1 in the degradation of various phthalates

Method used

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  • Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters
  • Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters
  • Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters

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Embodiment

[0031] Example 1 Isolation and identification of strains

[0032] Collect the activated sludge from the sewage treatment plant, weigh 5 g of the activated sludge sample into a 150 mL Erlenmeyer flask containing 50 mL of sterile water, and cultivate it at 30°C and 140 rpm for 3 days, then take 5 mL of the sludge suspension and add 100 mL PAEs (containing 50 mg / L DMP, DEP, DBP, DOP and DEHP respectively) in the above MSM medium. After culturing at 30°C and 140 rpm for 7 days, 1 mL inoculum was continuously enriched and transferred 10 times each time, and the content of PAEs in the medium was correspondingly increased to 800 mg / L. Then the 10 times acclimatized culture solution was diluted 10 3 ~10 5 Spread on LB solid plates and incubate upside down at 30°C for 1-3 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a strain of bacteria, numbered J-1. Then the strains were inoculated on LB solid ...

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Abstract

The invention belongs to the technical field of environment pollutant biological treatment, and specifically discloses applications of Microbacterium sp. J-1 in degradation of a plurality of phthalic acid esters (PAEs). The bacterial strain is obtained by the inventors of the invention for the first time via separation from activated sludge of sewage treatment plants, and is capable of realizing high efficiency degradation on a plurality of PAEs in polluted environment, and especially on short chain PAEs, so that application prospect of the bacteria strain on remediation of soil polluted by the plurality of PAEs is promising.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, in particular, relates to microbacteria ( Microbacterium sp.) Application of J-1 in the degradation of various phthalates. Background technique [0002] Numerous studies have shown that phthalic acid esters (PAEs) are highly toxic, teratogenic, carcinogenic, mutagenic, and reproductively toxic. PAEs are mainly used as plasticizers in the plastics industry, and have become one of the most common pollutants in the world. They are listed as priority pollutants by the US Environmental Protection Agency (EPA) and China Environmental Monitoring Station. According to the research results of the National Cancer Institute of the United States, dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl formate (DBP), butyl benzyl phthalate ( Six phthalates including BBP), di-n-octyl phthalate (DOP) and di-isooctyl phthalate (DEHP) are listed as priority polluta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34B01D53/84B01D53/72C12R1/01C02F101/34
CPCY02A50/20
Inventor 赵海明莫测辉蔡全英李彦文李慧冯乃宪杜欢陈学斌
Owner JINAN UNIVERSITY
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