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2,4-dinitrotoluene sulfonate efficient degradation strain Microbacterium sp.X3 and application thereof

A technology of dinitrotoluenesulfonate and bacterial strains, applied in the field of environmental biology

Active Publication Date: 2018-07-13
BEIJING INST OF COLLABORATIVE INNOVATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects and deficiencies of the existing TNT red water and TNT red water polluted soil remediation technology, to provide a 2,4-dinitrotoluenesulfonate efficient degradation bacteria and its application

Method used

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  • 2,4-dinitrotoluene sulfonate efficient degradation strain Microbacterium sp.X3 and application thereof
  • 2,4-dinitrotoluene sulfonate efficient degradation strain Microbacterium sp.X3 and application thereof
  • 2,4-dinitrotoluene sulfonate efficient degradation strain Microbacterium sp.X3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Degradation Ability Determination of 2,4-Dinitrotoluenesulfonate Degrading Strain Microbacterium sp.X3

[0033] Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days.

[0034]A certain concentration of bacterial suspension was inoculated into the above soil according to the inoculum amount of 2%. As for culturing in a constant temperature incubator at 30°C, samples were taken every 24 hours, and the concentrations of 2,4-DNT-3-SA and 2,4-DNT-5-SA were measured by high performance liquid chromatography. The degradation curve is attached Figure 4 shown. It can be se...

Embodiment 3

[0035] Embodiment 3: Microbacterium sp.X3 is to the degradation effect of sulfonate at different pH values

[0036] Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days. Weigh 20 g of the above soil into a conical flask, and use dilute H 2 SO 4 and NaOH to adjust the pH of the soil to be 3, 5, 7, 9, 11 respectively, add the bacterium liquid activated by the Microbacterium sp.X3 of OD600=1 according to the inoculum size of 5%, and adjust the water-soil ratio with the inorganic salt liquid medium to be 2: 5. Seal with breathable parafilm. All the Erlenmeyer flasks were placed in a co...

Embodiment 4

[0038] Embodiment 4: Microbacterium sp.X3 is to the degradation effect of sulfonate at different temperatures

[0039] Take uncontaminated soil, air-dry and grind, pass through a 1mm sieve, and set aside. A certain amount of 2,4-DNT-3-SA and 2,4-DNT-5-SA was weighed and dissolved in acetone. In the fume hood, the acetone solution of the two sulfonates was evenly sprayed into the above soil and stirred evenly. The soil concentration was made approximately 500 mg / kg each of 2,4-DNT-3-SA and 2,4-DNT-5-SA; it was allowed to air dry naturally in a fume hood for 2 days. Weigh 20g of the above soil into an Erlenmeyer flask, add Microbacterium sp.X3 activated bacterial solution with OD600=1 according to 10% inoculation amount, adjust the water-to-soil ratio to 2:5 with an inorganic salt liquid medium, and seal it with a gas-permeable sealing film . The Erlenmeyer flasks were placed in constant temperature incubators at 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C for a period of time, an...

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Abstract

The invention discloses a 2,4-dinitrotoluene sulfonate efficient degradation strain Microbacterium sp.X3 and application of the 2,4-dinitrotoluene sulfonate efficient degradation strain Microbacteriumsp.X3. The strain is preserved in the China General Microbiological Culture Collection Center on September 15, 2017, and the preservation number is CGMCC NO.14586. The invention further discloses a method for screening the 2,4-dinitrotoluene sulfonate efficient degradation strain and a use method applying the strain to degrading the TNT red water and soil polluted by the red water. The strain iscultured to the logarithmic phase and is then inoculated to the polluted soil, the concentrations of 2,4-DNT-3-SA and 2,4-DNT-5-SA in the polluted soil are respectively 500 mg / Kg, and the removal rateof two 2,4-dinitrotoluene sulfonates both reach 100% after the treatment for 4-6 days. The strain has excellent application in the remediation of the organic polluted soil polluted by nitrocompounds.

Description

technical field [0001] The invention belongs to the field of environmental biology, and specifically relates to the separation and application of a main pollutant 2,4-dinitrotoluenesulfonate degradation strain Microbacterium sp.X3 in TNT red water polluted soil Background technique [0002] TNT red water is a kind of TNT wastewater, which is produced in the TNT refining process. The crude TNT produced by the three-stage nitration method contains about 4.5% isomers, mainly 2,4,5- and 2,3,4-trinitrotoluene. In order to achieve military purity, the crude TNT is usually refined by sodium sulfite refining method to remove these isomers. Sodium sulfite can react with TNT isomers to generate 2,4-DNT-3-SA and 2,4-DNT-5-SA respectively, which are the main components in TNT red water. The seepage of TNT red water has caused serious soil pollution problems. [0003] Soil bioremediation technology originated in the 1980s. Compared with physical and chemical methods, bioremediation te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38C02F103/36
CPCB09C1/10C02F3/34C02F2101/003C02F2101/40C02F2103/36C12N1/205C12R2001/01
Inventor 叶正芳徐文杰李智林
Owner BEIJING INST OF COLLABORATIVE INNOVATION
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