Hydrogen-oxidizing bacteria having effect of promoting growth of plants and isolated culture and application of hydrogen-oxidizing bacteria

A technology of hydrogen oxidation and bacteria, which is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve the problems in the initial stage and the research on hydrogen oxidizing bacteria is not particularly in-depth, so as to increase production and promote Plant growth, enhance the effect of plant growth

Inactive Publication Date: 2019-09-13
NORTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research on hydrogen oxidizing bacteria has already started abroad in the late nineteenth century, so the separation methods and methods for hydrogen ox

Method used

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  • Hydrogen-oxidizing bacteria having effect of promoting growth of plants and isolated culture and application of hydrogen-oxidizing bacteria
  • Hydrogen-oxidizing bacteria having effect of promoting growth of plants and isolated culture and application of hydrogen-oxidizing bacteria
  • Hydrogen-oxidizing bacteria having effect of promoting growth of plants and isolated culture and application of hydrogen-oxidizing bacteria

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: the isolation culture of soil microorganism 1-0-19

[0034] 1. Sample collection: Soil samples were collected in Yaozhou District, Tongchuan City, Shaanxi Province in May, July, and September 2016. Several sampling points were selected in the field using the "S-shaped sampling method" to remove the topsoil and leaves. Residual branches, excavated below 10cm of the soil surface, collected the soil around the rhizosphere of alfalfa 5-15cm with a sealed bag, mixed the soil sample after the collection was completed, and divided the mixed soil sample into three parts, as three repetitions, liquid nitrogen After quick freezing, store in a -80°C refrigerator for later use.

[0035] The rhizosphere soil of non-legume plants was collected as a control in the nearby field according to the above method.

[0036] 2. Soil enrichment and separation

[0037] Accurately weigh 5.0 g of alfalfa soil samples, and put them into conical flasks filled with 45 mL of sterile wa...

Embodiment 2

[0045] The plant growth promoting effect of embodiment 2 hydrogen oxidizing bacteria 1-0-19

[0046] Select wheat seeds that are plump and free of pests, and of similar size, put them in water 5 times the amount of seeds, and stir gently for about 15 minutes. Then, soak in warm water at 30°C for 6 hours (the soaking time is appropriate, if the time is too long, the seeds will undergo anaerobic respiration, and the ethanol produced will damage the seeds and reduce the germination rate; if the time is too short, the inhibitors in the seeds cannot be leached) , the seed vigor is the highest at this temperature. Treat with 75% ethanol for 1 min, then rinse with sterile water to remove ethanol, then soak in sodium hypochlorite for 5 min, rinse with sterile water several times, and place the sterilized wheat seeds in a sterile petri dish (in a petri dish sterilized filter paper), put 15 grains in each petri dish, cover with clean and moist gauze, and germinate in the dark at 27°C f...

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Abstract

The invention discloses microbacterium Microbacterium1-0-19. The strain is isolated from a place nearby Tongchuan overpass, Yaozhou district, Tongchuan city, Shanxi province, and is classified into microbacterium bacteria based on physiological and biochemical characteristics and 16SrDNA analysis. Medicago sativa rhizosphere soil is used as a research object, and hydrogen-oxidizing bacteria are isolated through a continuous hydrogen inflating device; and through shape observation, physiological and biochemical identification and 16SrDNA identification of the hydrogen-oxidizing bacteria, the classification status of the hydrogen-oxidizing bacteria is determined. The optimal strain is fermented in a laboratory, and a hydrogen bacterium preparation is prepared. The biological preparation canbe used for agricultural production to promote growth of plants, increase yield, increase fertilizer effects and increase the stress tolerance capacity of the plants.

Description

technical field [0001] The present invention relates to the separation method and application of hydrogen oxidizing bacteria 1-0-19, in particular to a hydrogen oxidizing bacterium Microbacterium genus 1-0-19 ( Microbacterium sp. 1-0-19) and its microbial agent, the hydrogen oxidizing bacteria 1-0-19 strain was preserved in the Chinese Type Culture Collection Center of Wuhan University on September 19, 2018, and the preservation number is CCTCC No: M 2018638. Background technique [0002] In the long-term farming process, people have discovered the law that intercropping of leguminous crops can increase yield, but the physiological process and mechanism have not been deeply explored. At the beginning of the 20th century, Professor Dong Zhongmin and others discovered through experiments that the root nodules of leguminous plants will have H during nitrogen fixation. 2 released, but the content detected in the soil is little or even none, so it is inferred that this phenomen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01Y02A40/10
Inventor 王卫卫李璐璐刘瑞瑞李志英
Owner NORTHWEST UNIV
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