Hydrogen-oxidizing bacteria having effect of promoting growth of plants and isolated culture and application of hydrogen-oxidizing bacteria
A technology of hydrogen oxidation and bacteria, which is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve the problems in the initial stage and the research on hydrogen oxidizing bacteria is not particularly in-depth, so as to increase production and promote Plant growth, enhance the effect of plant growth
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Embodiment 1: the isolation culture of soil microorganism 1-0-19
[0034] 1. Sample collection: Soil samples were collected in Yaozhou District, Tongchuan City, Shaanxi Province in May, July, and September 2016. Several sampling points were selected in the field using the "S-shaped sampling method" to remove the topsoil and leaves. Residual branches, excavated below 10cm of the soil surface, collected the soil around the rhizosphere of alfalfa 5-15cm with a sealed bag, mixed the soil sample after the collection was completed, and divided the mixed soil sample into three parts, as three repetitions, liquid nitrogen After quick freezing, store in a -80°C refrigerator for later use.
[0035] The rhizosphere soil of non-legume plants was collected as a control in the nearby field according to the above method.
[0036] 2. Soil enrichment and separation
[0037] Accurately weigh 5.0 g of alfalfa soil samples, and put them into conical flasks filled with 45 mL of sterile wa...
Embodiment 2
[0045] The plant growth promoting effect of embodiment 2 hydrogen oxidizing bacteria 1-0-19
[0046] Select wheat seeds that are plump and free of pests, and of similar size, put them in water 5 times the amount of seeds, and stir gently for about 15 minutes. Then, soak in warm water at 30°C for 6 hours (the soaking time is appropriate, if the time is too long, the seeds will undergo anaerobic respiration, and the ethanol produced will damage the seeds and reduce the germination rate; if the time is too short, the inhibitors in the seeds cannot be leached) , the seed vigor is the highest at this temperature. Treat with 75% ethanol for 1 min, then rinse with sterile water to remove ethanol, then soak in sodium hypochlorite for 5 min, rinse with sterile water several times, and place the sterilized wheat seeds in a sterile petri dish (in a petri dish sterilized filter paper), put 15 grains in each petri dish, cover with clean and moist gauze, and germinate in the dark at 27°C f...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com