Mixed bacteria for promoting acacia nodulation and nitrogen fixation and application thereof

A technology of mixed bacteria and black locust, applied in the field of microorganisms, to achieve the effect of increasing nodulation rate and symbiotic nitrogen fixation, and promoting photosynthesis

Active Publication Date: 2019-12-03
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on the screening of suitable root nodule-promoting strains of leguminous vegetation for different vegetation.

Method used

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  • Mixed bacteria for promoting acacia nodulation and nitrogen fixation and application thereof
  • Mixed bacteria for promoting acacia nodulation and nitrogen fixation and application thereof
  • Mixed bacteria for promoting acacia nodulation and nitrogen fixation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Acquisition and identification of strains

[0031] Soil samples were collected from 5 cm of rhizosphere soil on the surface of slopes on both sides of Yueyang Avenue, Yueyang City, using the dilution coating plate method, in a 35 ° C incubator, with nutrient agar solid medium (NA medium: 10 g of peptone; 3 g of beef powder; Sodium chloride 5g; agar 15g; sterile water 1000mL) cultured for 2 to 3 days, picked different colonies by visual inspection, repeated streaking and purification to obtain several single colonies of different species.

[0032] Select a single colony to make a plate, and send it to Shanghai Jinyu Medical Laboratory for sequencing to obtain the 16S rDNA gene sequence, as shown in SEQID NO.1. The measured 16S rDNA gene sequence was compared with the sequence in the GenBank database by BLAST. The results showed that the similarity between the strain and Kocuria polaris was 99.32%. Combined with morphological characteristics and 16S rDNA gene sequenc...

Embodiment 2

[0039] 1. Cultivation of black locust seedlings

[0040] The cultivation of Robinia pseudoacacia seedlings was carried out in the greenhouse of the Baima teaching base of Nanjing Forestry University, with an air humidity of 65%; CO 2 The concentration is 450ppm; the maximum photosynthetically active radiation is 1850μmol / (m 2 s). Make sure that each pot receives the same light intensity and time every day. In order to prevent the rotten roots of Robinia pseudoacacia caused by too frequent watering, the weighing method was used for watering each time, so as to ensure that the soil water content of each pot reached 100% of the field water capacity.

[0041]1) Seed soaking: Soak the sieved black locust seeds with hot water at 60°C (seed: water=1:3), and after natural cooling for 24 hours, select the swollen seeds for germination, and soak the remaining seeds at 80°C. Continue to soak the seeds in hot water, and the ratio is the same as above. After 24 hours of natural cooling,...

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Abstract

The invention discloses mixed bacteria for promoting acacia nodulation and nitrogen fixation and an application thereof, which belong to the technical field of microorganisms. The mixed bacteria can be prepared from Kocuria sp. X-22, Microbacterium sp. X-26 and Bacillus sp. X-28, all the bacteria are preserved in the China Center for Type Culture Collection (CCTCC), the preservation numbers are CCTCC No: M 2019237, CCTCC No: M 2019238, and CCTCC No: M 2019239 respectively. The mixed bacteria are added to the periphery of the acacia seedlings, and compared with a single-bacteria treatment groupand a sterile treatment group, the synergistic superposition effect can be achieved, the nodulation rate and symbiotic nitrogen fixation level of acacia are obviously increased, and photosynthesis ofacacia is promoted.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a mixed bacterium for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and application thereof. Background technique [0002] Soil spraying technology is a greening technology that sprays the mixture of soil growth matrix materials and plant seeds evenly and at high pressure on rock and soil slopes. The growth matrix material mixture of this technology is prepared with screened soil bacteria, which is mainly a greening technology developed for hard slopes such as rocks and for plants to create a suitable growth environment on hard slopes. As one of the important components of the growth matrix material of this technology, the screened soil bacteria play two roles: one is to respond to the change of soil ecological mechanism and environmental stress, accelerate the erosion of rock mass, and effectively improve the Substrate interface fusion; t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01C12R1/07
CPCC12N1/20C12N1/205C12R2001/01C05G3/90A01N63/22C12R2001/07A01N25/02C12N2500/12C12N2500/74C12N2501/998C12N2523/00C12N2527/00
Inventor 庄家尧刘超王晓雪郑佳欣田琨
Owner NANJING FORESTRY UNIV
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