Bacterial strain for treating high concentrated organic wastewater, microorganism bacterium agent and application thereof

A technology of microbial inoculants and strains, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problems of long system recovery process, high salt content, complex components, etc., to achieve impact resistance The effect of strong ability, high organic load and simple operation

Active Publication Date: 2016-03-02
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Application Information

AI Technical Summary

Problems solved by technology

Oil refining, chemical industry and other industries produce a large amount of high-concentration organic wastewater. This type of wastewater not only contains high-concentration refractory organic pollutants, but also has complex components and high salt content. The recovery value of this type of wastewater is not high. It is very high, so it brings great difficulty

Method used

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  • Bacterial strain for treating high concentrated organic wastewater, microorganism bacterium agent and application thereof
  • Bacterial strain for treating high concentrated organic wastewater, microorganism bacterium agent and application thereof
  • Bacterial strain for treating high concentrated organic wastewater, microorganism bacterium agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation method of embodiment 1 microbial bacterial agent and the application in phenol acetone wastewater

[0020] The bacterial strains Y-1 and Y-3 of the present invention are obtained from mixed sludge samples of industries such as oil refining, chemical industry, medicine and dyestuffs through domestication, cultivation, screening and separation of mixed high-concentration organic wastewater.

[0021] The 16SrDNA identification of strain Y-1 and strain Y-3 were carried out respectively, and the specific steps were as follows:

[0022] Use forward primer F1: 5'-AGAGTTTGATCCTGGCTCAG-3', reverse primer

[0023] R1:5'-AAGGAGGTGATCCAGCCGCA-3' was subjected to DNA amplification (PCR). PCR reaction conditions: pre-denaturation at 95°C for 5 min, denaturation at 95°C for 45 s, annealing at 55°C for 45 s, extension at 72°C for 90 s, 30 cycles, final extension at 72°C for 10 min, storage at 4°C. PCR products were separated by 0.8% agarose gel, purified and recovered...

Embodiment 2

[0044] Application of embodiment 2 Gordonella Y-1 single strain preparation in high concentration phenol acetone wastewater

[0045] The difference from Example 1 is that the high-efficiency bacterial agent is composed of a single strain of Gordonia Y-1.

[0046] See Example 1 for the cultivation method, scale-up fermentation conditions and bacterial agent preservation of seed liquid Y-1 to obtain 2×10 9 CFU / mL microbial fermentation broth. In order to facilitate the comparison of experimental results, this example still applies bioaugmentation to high-concentration phenol-acetone wastewater.

[0047] Phenol-acetone wastewater was diluted 5 times and adjusted to pH=7.0. Nitrogen and phosphorus were supplemented according to COD:N:P=500:5:1. It was treated in an ordinary activated sludge system with a residence time of 32 hours. The high-efficiency bacteria prepared by the above method The biochemical system can be quickly started with the reagent, and the treatment status of...

Embodiment 3

[0049] The preparation method of embodiment 3 microbial bacterial agents and the application in acrylic acid production wastewater

[0050] The difference from Example 1 is that the experimental water is high-concentration acrylic acid wastewater, the composition ratio of high-efficiency bacterial agents is different, the culture conditions are different, the total number of colonies contained in the bacterial liquid is higher, and the preservation methods of the bacterial agents are also different.

[0051] 1. Preparation of seed solution

[0052] The seed liquid preparation method of Y-1 and Y-3 is the same as example 1.

[0053] 2. Fermentation tank fermentation

[0054] The Y-1 and Y-3 seed liquids obtained from the above culture were mixed according to the ratio of 1:3, and moved to the fermenter according to the inoculation amount of 5% of the total amount of the fermented liquid, and the mixed fermentation culture was carried out. The conditions were: tank temperature ...

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Abstract

The invention discloses a bacterial strain for treating high concentrated organic wastewater, a microorganism bacterium agent and application thereof. The bacterial strain specifically comprises Gordonia sp. Y-1 (the preservation registration number is CGMCC NO.10791) and Microbacterium sp. Y-3 (the preservation registration number is CGMCC NO.10792), and the Gordonia sp. Y-1 and the Microbacterium sp. Y-3 are mixed according to the proportion of 1:1-3 to perform magnifying fermentation so as to obtain the bacterium agent with the concentration of 1*109-1*1011 CFU/mL. The Y-1 and the Y-3 of the bacterial strain and the microorganism bacterium agent can maintain the better organic matter degradation capacity and can be used in the treatment field of pollutant reduction and degradation of high concentrated organic wastewater.

Description

technical field [0001] The invention relates to a bacterial strain for treating high-concentration organic wastewater, a microbial bacterial agent and an application thereof, which belong to the technical field of environmental protection and also belong to the category of biological enhancement technology. Background technique [0002] Biological treatment is one of the most commonly used methods for wastewater treatment. It has the advantages of wide application range, strong adaptability and low operating cost. Oil refining, chemical industry and other industries produce a large amount of high-concentration organic wastewater. This type of wastewater not only contains high-concentration refractory organic pollutants, but also has complex components and high salt content. The recovery value of this type of wastewater is not high. It is very high, so it brings great difficulty to the efficient and stable operation of the preferred biochemical treatment process. [0003] Th...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34
Inventor 顾锡慧官赟赟雷太平李亮张艳芳滕厚开
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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