Microbacterium and microbial reduction method thereof for repairing chromium-polluted soil

A technology for chromium-contaminated soil and microbacteria, applied in the field of microorganisms, can solve problems such as the inability to meet the pollution prevention and control needs of chromium-contaminated sites, the differences in site soil quality and groundwater characteristics, and the complex and diverse laws of the migration and transformation of chromium pollutants, so as to achieve sustainable development. , the effect of high reduction efficiency and good industrial application prospects

Pending Publication Date: 2022-06-24
有研资源环境技术研究院(北京)有限公司 +1
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Problems solved by technology

In addition, my country has a vast territory, and the soil quality and groundwater characteristics of the site are significantly different, which makes the migration and transformation of chromium pollutants in different sites complex and diverse.
In terms of remediation of c

Method used

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  • Microbacterium and microbial reduction method thereof for repairing chromium-polluted soil
  • Microbacterium and microbial reduction method thereof for repairing chromium-polluted soil

Examples

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Effect test

Embodiment 1

[0034] Example 1 Isolation of Chromium Reducing Bacteria Microbacterium

[0035] 1) Add 5g of soil sample from chromium-contaminated site to 95mL of deionized water, place it on a shaker at 30°C and 100rpm for 15 minutes, take it out and stand still for 30 minutes, and collect the supernatant;

[0036] 2) Add 1 mL of the above-mentioned supernatant to 9 mL of separation medium without agar powder, and place it in a shaking table at 30°C and 150 rpm for 7 days;

[0037]3) Add 1 mL of the above-mentioned cultured bacterial liquid to 9 mL of sterile water, shake well, and obtain 10 -1 Gradient dilutions, dilute 10 in sequence -2 to 10 -5 Spread evenly on the separation medium plate, invert and cultivate at 30°C for 7 days, pick up the single colony on the plate for four-line three-zone streak culture;

[0038] 4) Through multiple transfer and separation and purification, a single colony with the same colony characteristics is obtained, such as figure 1 shown. from figure 1 ...

experiment example 2

[0041] Experimental Example 2 Microbial Remediation Method of Chromium Contaminated Soil

[0042] The components of the medium used in the repair are: glucose 4.0g / L, corn steep liquor dry powder 5.0g / L, Na 2 HPO 4 3.0g / L, KH 2 PO 4 3.0g / L, NaCl 0.5g / L, MgSO 4 .7H 2 O 0.246g / L, CaCl 2 0.5g / L, pH 7.

[0043] The contaminated soil used in the restoration was collected from the chromium-contaminated site of the Haibei Chemical Plant in Qinghai. The pollution status: total chromium 470mg / kg, hexavalent chromium 430mg / kg.

[0044] 1) Prepare 150 mL of acclimation medium for sterilization, pick up Microbacterium sp colonies on the plate into the acclimation medium, shake at 30° C. and 150 rpm overnight to form an activated GRINML SWG1 bacterial solution;

[0045] 2) Add 5% of the activated GRINML SWG1 bacterial solution to K 2 Cr 2 O 7 In the acclimation medium with a content of 400 mg / L, after culturing for 3 weeks at 30 ° C and 150 rpm, take 5% of the inoculum of the ...

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Abstract

The invention provides a strain of microbacterium, the classification name of the microbacterium is Microbacterium sp.GRINML SWG1, the preservation unit is China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is August 6, 2021, and the preservation number is CCTCC NO: M2021992. The microbacterium provided by the invention can efficiently reduce soluble hexavalent chromium with different concentrations, and provides an experimental technology and a theoretical basis for applying a microbial reduction technology to chromium-contaminated soil remediation.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbacterial strain and a microbial reduction method for in-situ repairing of chromium-contaminated soil. Background technique [0002] my country is a major producer of chromium salt chemical industry, which has caused serious chromium pollution in the stacking of chromium slag and the leftover site of the chromium salt factory. In addition, my country has a vast territory, and the soil and groundwater characteristics of the sites are significantly different, which makes the migration and transformation laws of chromium pollutants in different sites complex and diverse. In terms of remediation of chromium-contaminated sites, most of the remediation projects currently use solidification / stabilization and barrier landfill technologies, and traditional remediation technologies are far from meeting the pollution prevention and control needs of chromium-contami...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36B09C1/10C12R1/01
CPCC12N1/20C12N1/36B09C1/10
Inventor 李红霞崔兴兰钟娟王雷车小奎徐政郑其袁学韬
Owner 有研资源环境技术研究院(北京)有限公司
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