Microbacterium sp. strain and application thereof

A technology of microbacteria and strains, applied in the field of microorganisms, can solve the problems of long cycle, low economy, low tolerance of high-concentration phenol-containing wastewater, etc.

Active Publication Date: 2015-02-25
中国中化股份有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the existing phenol-degrading strains obtained through screening generally have the problem of low tolerance to high-concentration phenol-containing wastewater
Generally, the tolerance concentration of phenol-degrading strains to phenol is lower than 2000 mg / L, and the phenol degradation rate is usually lower than 90% in the presence of high-concentration phenol; The treatment effect of high degradation rate requires a long cycle, and the economy is low in the treatment of actual wastewater

Method used

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  • Microbacterium sp. strain and application thereof
  • Microbacterium sp. strain and application thereof
  • Microbacterium sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) It is obtained from the activated sludge of a biochemical aerobic pool of a petrochemical plant in Liaoning by enrichment culture method, and the specific steps are as follows.

[0024] (1) Preparation of culture medium: 100 mL of phenol-inorganic salt medium mixed system was placed in a 250 mL Erlenmeyer flask, including culture medium solution, inorganic salt trace element solution and phenol. Add 1mL trace element solution per 1L of culture medium; phenol is first dissolved in a water bath at 45°C, and added to the system after autoclaving the inorganic salt culture medium.

[0025] Medium solution (g / L): 0.9KH 2 PO 4 , 6.5Na 2 HPO 4 12H 2 O, 0.4 (NH 4 ) 2 SO 4 , 0.2MgSO 4 ·7H 2 O; trace element solution (g / L): 1g FeSO 4 ·7H 2 O, 1g MnSO 4 ·H 2 O, 0.25g Na 2 MoO 4 2H 2 O, 0.1g H 3 BO 4 , 0.25g CuCl 2 2H 2 O, 0.25g ZnCl 2 , 0.1gNH 4 ·VO 3 , 0.25g Co(NO 3 ) 2 ·6H 2 O, 0.1g NiSO 4 ·6H 2 O, dissolved in 900 mL of distilled water, added 5 m...

Embodiment 2

[0044] At pH 7.0, temperature 30°C, initial concentrations of phenol were 500mg / L, 1000mg / L, 1500mg / L, 2000mg / L, 2500mg / L, 3000mg / L, 3500mg / L, 4000mg / L, SY-PD The culture of the -42 strain was inoculated in 100 mL of the above-mentioned inorganic salt medium with phenol as the sole carbon source, cultured with shaking at 150 r / min for 48 hours, and then sampled.

[0045] The content of residual phenol in the sample was determined by 4-aminoantipyrine extraction spectrophotometry, and the degradation rate was calculated. Taking the initial concentration of phenol as the abscissa, taking the phenol degradation rate and OD600 value (cell concentration) as the ordinate, draw the degradation and cell concentration curves, and the results are shown in figure 1 .

[0046] from figure 1 It can be seen that Microbacterium SY-PD-42 strain has a good degradation effect on phenol when the phenol concentration is lower than 2000mg / L, and the degradation rate can reach more than 99%. Wel...

Embodiment 3

[0050] The culture of Microbacterium SY-PD-42 strain was inoculated in 100 mL of the above-mentioned inorganic salt medium with phenol as the sole carbon source, the pH of the medium was 7.0, and the initial concentration of phenol was 2000 mg / L. Min shaking culture, sampling every 6h.

[0051] Using 4-aminoantipyrine extraction spectrophotometry, measure the residual phenol content in the sample, take time as the abscissa, and the phenol residual concentration as the ordinate, draw the degradation curve, and the results are shown in figure 2 . from figure 2 It can be seen that when the initial phenol concentration of the medium is 2000mg / L, the Microbacterium SY-PD-42 bacterial strain of the present invention can degrade more than 99% of the phenol in the medium within 48h, and the final phenol concentration in the culture solution is lower than 1mg / L .

[0052] The Microbacterium strain SY-PD-42 of the present invention can be used to treat phenol-containing solutions t...

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Abstract

The invention relates to the technical field of microbes, and concretely relates to a microbacterium sp. strain and a biological pure culture thereof, and application of the bacterial strain to degrade phenol. The microbacterium sp. strain is SY-PD-42, and has the preservation number of CCTCC NO: M2014101. The microbacterium sp. strain is capable of efficiently degrading phenols in wastewater, and also is capable of tolerating a phenol-containing solution with the phenol concentration up to 2500 mg / L.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a microbacteria strain, its biologically pure culture, and its application in degrading phenol. Background technique [0002] Phenol is a common water-soluble organic pollutant that widely exists in the wastewater discharged from petroleum, chemical, gas, coking, steel and phenol production plants. Phenol-containing wastewater has a wide range of sources, a large amount, and great harm. Discharging into water bodies will cause serious pollution to water bodies. It is one of the toxic and harmful wastewater listed as a key solution in my country's water pollution control. [0003] At present, there are mainly physical methods, chemical methods, and biological methods to treat phenol-containing wastewater at home and abroad. Among them, the biological method uses microbial metabolic activities to remove toxic substances in wastewater, and has the advantages of no secondary pollut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
CPCC02F3/34C02F2101/345C12N1/20C12N1/205C12R2001/01
Inventor 朱希坤李小明戴速航杨德玉周悦李旭
Owner 中国中化股份有限公司
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