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Microbacterium for cassava nitrogen fixation and preparation method and application thereof

A technology of microbacteria and cassava, applied in the field of nitrogen-fixing microbacteria of cassava and its preparation, can solve the problems of diminishing returns, environmental pollution and the like, and achieve the effect of improving the accumulation of nitrogen

Active Publication Date: 2016-08-31
GUANGXI UNIV
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Problems solved by technology

However, the excessive dependence of my country's agriculture on nitrogen fertilizer will inevitably lead to the risk of diminishing returns and environmental pollution, such as soil acidification, salinization, water eutrophication, and global greenhouse effect.

Method used

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  • Microbacterium for cassava nitrogen fixation and preparation method and application thereof
  • Microbacterium for cassava nitrogen fixation and preparation method and application thereof
  • Microbacterium for cassava nitrogen fixation and preparation method and application thereof

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experiment example 2

[0061] The present invention is that the endophytic nitrogen-fixing bacteria A08 strain belongs to Firmicutes (Microbacterium sp.). Deposit unit: General Microorganism Center (CGMCC), China Committee for Microorganism Culture Collection, deposit date: March 07, 2016, deposit number: 12182;

[0062] The biological bacterial agent with microbacteria as the active ingredient in the present invention is a biological bacterial agent prepared from the above bacterial strains through tube seed cultivation and liquid fermentation.

[0063] The preparation method of the biological bacterial agent of the present invention includes tube culture and liquid fermentation experiment methods. Specifically include:

[0064] A: Tube seed culture: Inoculate A08 on the slant medium of the test tube, and culture it at a constant temperature of 28-32°C for 2-3 days to obtain it.

[0065] B: Liquid fermentation: Inoculate the test tube seed on the liquid medium, and culture it on a shaker at 28-32...

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Abstract

The invention discloses microbacterium for cassava nitrogen fixation and a preparation method and application thereof. The microbacterium belongs to Microbacterium sp. and is preserved in CGMCC (China General Microbiological Culture Collection Center) on March 7, 2016, and a preservation serial number is CGMCC NO.12182. A biological agent with the microbacterium as an active component is prepared by tube culture and liquid fermentation culture. By application of the microbacterium and the biological agent thereof, nitrogen contents of roots, stems and leaves of cassava seedlings can be remarkably increased, nitrogen accumulation in an early growth stage of cassava is benefited, and high application value in cassava production is achieved.

Description

technical field [0001] The invention belongs to the field of nitrogen-fixing bacteria in the technical field of microorganisms, and in particular relates to a microbacterium for cassava nitrogen-fixing, a preparation method and application thereof. Background technique [0002] Plant endophytic nitrogen-fixing bacteria are a group of microorganisms that colonize plants and can jointly fix nitrogen with host plants. The use of endogenous nitrogen-fixing bacteria can convert free nitrogen in the atmosphere into nitrogen that plants can directly absorb and use, and then further transform into protein, nucleic acid and other macromolecular substances needed by plants to supply the needs of plant growth. From an agricultural point of view, the use of biological nitrogen fixation is one of the important ways to provide nitrogen fertilizer for crops. Since the 1980s, endophytic nitrogen-fixing bacteria have become a research hotspot at home and abroad because of their nitrogen-fix...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C05F11/08C12R1/01
CPCC05F11/08C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 何冰张晓顾明华温荣辉
Owner GUANGXI UNIV
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