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Microbial strain and application thereof

A technology of microorganisms and strains, applied in the direction of microorganisms, microorganism-based methods, applications, etc., to achieve the effects of alcohol and cigarette smoke, reduced irritation, and mild reaction conditions

Active Publication Date: 2012-10-10
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the reported nicotine-degrading bacteria, there is no report of using Microbacterium to degrade nicotine

Method used

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  • Microbial strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Slant culture: 10g peptone, 5g yeast extract, 10g sodium chloride, 15g agar, 1000ml distilled water, pH7.0, inoculate the cryopreserved glycerol suspension on the slant medium, and incubate at 30°C for 2 days.

[0030] Shake flask culture: nicotine 1g, peptone 5g, yeast extract 5g, sodium chloride 5g, K 2 HPO 4 ·3H2 O 13.3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 0.2g, 0.5ml of trace elements, add 1000ml of distilled water, adjust the pH value to 7.0, and sterilize with high pressure steam. A 250ml shaker flask has a liquid volume of 30ml, an inoculum size of 3v / v%, a culture temperature of 20°C, a shaker rotation speed of 200rpm, and shaker flask culture for 24 hours, and the nicotine degradation rate reaches 100%. Trace elements: CaCl 2 0.04g, CuSO 4 0.07g, MnSO 4 ·H 2 O 0.008g, FeSO 4 ·7H 2 O 0.004g, Na 2 MoO 4 2H 2 O 0.1g, add 0.1mol·L -1 Dissolve in HCl to a volume of 100ml.

Embodiment 2

[0032] The slant culture was the same as in Example 1. Fill 30ml medium in a 250ml shaker flask, its composition is as follows: nicotine 4g, corn steep liquor 15g, yeast extract 5g, sodium chloride 5g, K 2 HPO 4 ·3H 2 O 13.3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 0.2g, 0.5ml of trace elements, add 1000ml of distilled water, adjust the pH value to 7.0, and sterilize with high pressure steam. A 250ml shake flask has a liquid volume of 30ml, an inoculum size of 2v / v%, a culture temperature of 30°C, a shaker rotation speed of 200rpm, and shake flask culture for 48 hours, and the nicotine degradation rate reaches 85%.

Embodiment 3

[0034] The slant culture was the same as in Example 1. Fill 30ml medium in a 250ml shaker flask, its composition is as follows: nicotine 8g, corn steep liquor 15g, yeast extract 5g, sodium chloride 5g, K 2 HPO 4 ·3H 2 O 13.3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O 0.2g, 0.5ml of trace elements, add 1000ml of distilled water, adjust the pH value to 7.0, and sterilize with high pressure steam. A 250ml shaker flask has a liquid volume of 30ml, an inoculum size of 2v / v%, a culture temperature of 40°C, a shaker rotation speed of 200rpm, and shaker flask culture for 72 hours, and the nicotine degradation rate reaches 62%.

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Abstract

The invention discloses a microbial strain and an application thereof, particularly relates to a tobacco nicotine degrading microbacterium GYC29 (Microbacterium sp.GYC29) with CCTCC of NO:M2010311 and an application of the microbacterium in tobacco. The strain disclosed herein can decompose and utilize nicotine in the growth process. According to the invention, by adding the ferment liquor of the strain or thallus with the additional amount of 1-5 % of the weight of the tobacco leaves in the tobacco with the water content of 10-50 % and fermenting for 6-72 h, the nicotine content of the tobacco leaves is reduced by 2-33%, the irritation of the tobacco leaves is obviously reduced, the miscellaneous gases are reduced, the smoke becomes pure and mild, and the sensory quality is obviously improved; the degradation of tobacco nicotine is realized by using microbes, the nicotine content of tobacco raw materials can be regulated properly, and the applicability of the tobacco leaves is raised.

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a tobacco nicotine-degrading bacterium—Microbacterium sp. GYC29 (CCTCC NO: M2010311) and its application in tobacco, belonging to the technical field of microbial application. Background technique [0002] Nicotine, also known as nicotine, is the main component of tobacco alkaloids. The content of nicotine in flue-cured tobacco is generally 1.5% to 3.5%, preferably about 2.5%. If the nicotine content is too low, the energy will be too small; if the nicotine content is too high, the energy will be too large, which will cause a tingling and spicy feeling. To reduce the nicotine content in tobacco, there are mainly three approaches at present: (1) regulate and control from the perspective of agricultural planting: mainly control from aspects such as genetics, ecology and cultivation. (2) From a chemical point of view: the alkaloids in the tobacco leaves can be removed from...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/01
Inventor 龙章德黄泰松韦建玉白森邹克兴胡亚杰蔡联合金亚波张纪利
Owner CHINA TOBACCO GUANGXI IND
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