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Microbacterium sp.T3 with tolerance on heavy metals and application thereof

A heavy metal and tolerance technology, applied in the direction of bacteria, water pollutants, water/sludge/sewage treatment, etc., can solve the problem of less bacteria, achieve the effect of strong vitality, many types of metal tolerance, and simple operation

Active Publication Date: 2011-07-20
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic and foreign scholars' research work on biosorption mainly focuses on fungi, algae, yeast, etc., while there are few domestic and foreign reports on bacteria as biosorbent materials.

Method used

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  • Microbacterium sp.T3 with tolerance on heavy metals and application thereof
  • Microbacterium sp.T3 with tolerance on heavy metals and application thereof
  • Microbacterium sp.T3 with tolerance on heavy metals and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: strain morphological characteristics and biological identification

[0034] 1. Morphological characteristics

[0035] Streak inoculate the monoclonal colony into LB solid medium, place the plate upside down in a constant temperature incubator, incubate at 30°C for 1 to 3 days, and observe the colony morphology. Microbacterium sp.T3 round spots, bright yellow, smooth and moist surface, no diffusion, see figure 1 .

[0036] 2. Physiological and biochemical characteristics

[0037] Gram staining, oxidase test, contact enzyme test, bacterial motility test, oxidative fermentation test of sugar alcohols (including glucose, lactose, galactose, fructose, xylose, sucrose, maltose, mannitol), utilization of sugar Alcohol gas production test, methyl red test, V-P test, indole test, nitrate reduction test, H 2 S production test, arginine dihydrolase test, citrate utilization test, urease test, starch hydrolysis, litmus milk test, gelatin liquefaction test, the result...

Embodiment 2

[0042] Example 2: Bacteria to the maximum tolerance concentration of heavy metals

[0043]Prepare solid medium containing heavy metal ions, the concentration starting from 0.1mM / L and increasing by 0.5mM. Streak inoculation on fresh LB solid medium, culture at 30°C for 48h for activation, pick single clone colony and inoculate into LB liquid medium, culture at 30°C, 120r / min shaking for 24h, collect bacterial concentration OD 600 1.5 bacterial solution by 10 0 、10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Proportionally dilute, dip the bacterial solution of each concentration and inoculate it on the heavy metal solid medium. Cultivate at 30°C, observe and record the growth of the bacteria every 24 hours, and the lowest heavy metal concentration that inhibits its obvious growth within 120 hours is the maximum tolerated concentration of the bacteria, as shown in Table 2.

[0044] Table 2

[0045]

Embodiment 3

[0046] Embodiment 3: The adsorption effect of adsorbent to lead

[0047] Be respectively 20, 50, 100, 150 and 200mg / L in lead concentration, in the solution that pH value is 6, respectively add and cultivate 12h, the adsorbent that dosage is 0.5g / L is made by the bacterium of the present invention, in Adsorbed at 30°C for 90 minutes, centrifuged to collect the supernatant, and measured the remaining lead ion concentration at adsorption equilibrium. With Pb 2+ The increase in initial mass concentration, Pb 2+ The removal rate decreased, while the equilibrium adsorption capacity increased, but the increase in adsorption capacity became smaller and smaller. The maximum adsorption capacity of biosorbent can reach 87.49mg / g, see image 3 shown.

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Abstract

The invention discloses a Microbacterium sp.T3 with tolerance on heavy metals and application thereof. The Microbacterium sp.T3 is preserved with the number of CGMCCNo.4282. The bacterium can tolerate a plurality of heavy metals such as Pb<2+>, Cr<6+>, Mn<2+>, Zn<2+>, Cu<2+>, Ni<2+>, Cd<2+>, Co<2+>, Ag<+>, Hg<2+>, and the like, particularly has the tolerance concentration on the Pb<2+> and the Mn<2+> respectively reaching 2000mg / L and 1000mg / L. Strains are activated, cultured, collected, cleaned and dried to prepare an adsorbent in which heavy metals are removed. The maximum adsorption capacity on Pb<2+> as one of the heavy metals can reach 87.49mg / L.

Description

technical field [0001] The invention relates to a bacterial strain tolerant to multiple heavy metals and an application method for preparing a biosorbent by using the bacterial strain. Background technique [0002] The development of industrialization has brought severe heavy metal pollution, which poses a huge threat to the ecological environment and human health due to its non-degradable and bioaccumulative properties. Traditional wastewater treatment methods mainly include precipitation, adsorption, electrolysis, and membrane separation. However, these methods generally have disadvantages such as high investment cost, secondary pollution, and poor treatment effect on trace heavy metals. Due to its low cost and wide range of material sources, biosorption has gradually become a research hotspot. [0003] The microorganisms isolated and screened from soil polluted by heavy metals, due to the stress of heavy metals in the living environment, can not only have a certain tole...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/20
Inventor 柴立元杨志辉苏艳蓉陈跃辉闵小波
Owner CENT SOUTH UNIV
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