Application of sophora tonkinensis endogenetic bacterium B22 in prevention and treatment of panax notoginseng black spot
A technology of endogenous bacteria and B22, applied in the biological field, can solve problems such as threats to health, pollution, and pesticide residues in Panax notoginseng products, and achieve strong inhibitory effects and broad application prospects
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Embodiment 1
[0022] Isolation, screening and identification of strains
[0023] 1. Isolation of strains
[0024] Test material: wild Vietnamese pagoda tree collected from the limestone area of Tiandeng County, Guangxi.
[0025] Tested strains: provided by Institute of Plant Pathology, Guangxi University.
[0026] Medium: 1000ml NA medium: 3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, 15g of agar, pH7.0.
[0027] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dr...
Embodiment 2
[0079] Inhibitory Effects of Strain Metabolites on Panax notoginseng Black Spot
[0080] 1. Fermentation culture of bacterial strain B22 and extraction of metabolites of bacterial strain B22
[0081] Inoculate Sophora vietnamese endophyte B22 into a 2-liter Erlenmeyer flask containing 1000ml of NB liquid medium (3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, pH7.0) 130r / min shaker fermentation culture for 10 days, the obtained fermented product was concentrated and dried under reduced pressure, added 2 times the methanol of the fermented product and ultrasonically ultrasonicated for 40 minutes, ultrasonically centrifuged, and the supernatant obtained after centrifugation was collected and concentrated under reduced pressure to obtain the strain The methanol crude extract of the fermented product is the metabolite of endophytic bacteria B22 of Sophora vietnamese.
[0082] 2. Inhibitory effect of endophytic B22 metabolites of Sophora japonica on mycelia...
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