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Method for inducing hairy roots of sophora tonkinensis roots

A mountain bean and root hair technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of endangered depletion of wild resources, rising prices of medicinal materials, etc. Effect

Active Publication Date: 2015-07-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the increase in the amount of medicines and the expansion of the way of use, the market demand for mountain bean root is constantly increasing, and the price of medicinal materials is also rising, which leads to the uncontrolled mining of mountain bean root resources, and the wild resources are on the verge of exhaustion

Method used

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  • Method for inducing hairy roots of sophora tonkinensis roots
  • Method for inducing hairy roots of sophora tonkinensis roots
  • Method for inducing hairy roots of sophora tonkinensis roots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] This example is to test the influence of different pre-cultivation time on the induction of hairy roots of the bean root.

[0018] A method for inducing hairy roots of Soba root, comprising the following steps:

[0019] (1) Seed germination: Take the seeds of Soba root, sterilize them with 0.1% mercuric chloride for 10 minutes, wash them with sterile water for 3 times, remove the surface moisture with sterile filter paper, inoculate them into MS seed medium without adding any hormones, and place them in Germination culture was carried out in the cultivation room for 15 days to induce seed germination to obtain seedlings, wherein 30 g / L sucrose and 3.4 g / L agar were added to the MS seed medium, and the pH value of the medium was 5.8;

[0020] (2) Explant pre-cultivation and cultivation: the seedlings obtained by seed germination in step (1) are cut into 1-2cm fritters according to the cotyledons, scratch the wounds on the fritters with a scalpel, and inoculate into the s...

Embodiment 2

[0029] This example is to test the effect of different Agrobacterium rhizogenes on the induction of hairy roots of bean root.

[0030] A method for inducing hairy roots of Soba root, comprising the following steps:

[0031] (1) Seed germination: Take the seeds of Soba root, sterilize them with 0.1% mercuric chloride for 10 minutes, wash them with sterile water for 3 times, remove the surface moisture with sterile filter paper, inoculate them into MS seed medium without adding any hormones, and place them in Germination culture was carried out in the cultivation room for 15 days to induce seed germination to obtain seedlings, wherein 30 g / L sucrose and 3.4 g / L agar were added to the MS seed medium, and the pH value of the medium was 5.8;

[0032] (2) Pre-cultivation of explants: the seedlings obtained by seed germination in step (1) are cut into small pieces of about 1-2cm according to the cotyledons, scratch the wound on the small piece with a scalpel, and inoculate until no h...

Embodiment 3

[0041] This example is to test the effect of different explants on the induction of hairy roots of the bean root.

[0042] A method for inducing hairy roots of Soba root, comprising the following steps:

[0043] (1) Seed germination: Take the seeds of Soba root, sterilize them with 0.1% mercuric chloride for 10 minutes, wash them with sterile water for 3 times, remove the surface moisture with sterile filter paper, inoculate them into MS seed medium without adding any hormones, and place them in Germination culture was carried out in the cultivation room for 15 days to induce seed germination to obtain seedlings, wherein 30 g / L sucrose and 3.4 g / L agar were added to the MS seed medium, and the pH value of the medium was 5.8;

[0044] (2) Explant pre-cultivation: the seedlings obtained by seed germination in step (1) are cut into small pieces of about 1-2cm according to cotyledons, hypocotyls, blades, and stem segments, and are drawn on the small pieces with a scalpel Wounds wer...

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Abstract

The invention discloses a method for inducing hairy roots of sophora tonkinensis roots. The method comprises the following steps: (1) disinfecting sophora tonkinensis seeds, and then inoculating to an MS seed culture medium for germinating; (2) cutting the germinated seeds into chips of 1-2cm according to cotyledons, hypocotyls, leaves and stems, scratching with a scalpel, and inoculating to an MS explant culture medium for pre-culturing; (3) soaking the pre-cultured material into rooting agrobacterium liquid for bacterial infection; (4) inoculating the material subjected to bacterial infection to an MS co-culture medium for co-culturing; and (5) inoculating the co-cultured material to a bacteriostatic culture medium for bacteriostatic culture and sterilization. By adopting the method for inducing the hairy roots, the obtained hairy roots have the characteristics of high growth speed, good genetic stability, strong synthetic capability and capability of stably releasing part of metabolites to the culture medium; and the method is simple and convenient in operation, fast and high in induction rate, and can be used for producing industrial replaceable resources of sophora tonkinensis roots in large scale.

Description

technical field [0001] The invention relates to a method for plant biological induction, in particular to a method for inducing hairy roots of bean root. Background technique [0002] Shandougen, also known as Guangdougen and Nandougen, is the dry root and rhizome of the leguminous plant Sophora tonkinensis Gapnep. It is distributed in southern my country, with the largest output in Guangxi. It has the effects of clearing fire and detoxifying, reducing swelling and relieving pain, etc. It can treat sore throat, sore throat, sore throat, swelling and pain of gums, hot cough with panting, hepatitis, constipation, jaundice, diarrhea, hemorrhoids, bald sores, scabies and mosquitoes and insects , Dog bites, etc. The pharmaceutical industry uses mountain bean root as a raw material to develop a large number of injections for hepatitis, tablets for sore throat, and anti-tumor Chinese patent medicines. At the same time, it is also used in the industry to extract matrine, oxymatrine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦范韦坤华李林轩缪剑华姚绍嫦谢代祖蒋妮林杨
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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