Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng anthrax
A technology of endophytic fungi, Fusarium solani, applied in the biological field, can solve problems such as pollution, health threats, production reduction of Panax notoginseng, etc., and achieve broad application prospects and strong inhibitory effect
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Embodiment 1
[0023] Isolation, screening and identification of strains
[0024] 1. Isolation of strains
[0025] Test material: wild Vietnamese pagoda tree collected from the limestone area of Tiandeng County, Guangxi.
[0026] Tested strains: provided by Institute of Plant Pathology, Guangxi University.
[0027] Medium: 1000ml potato dextrose agar medium (PDA medium), PDA medium contains 300g of potatoes, 20g of glucose, 20g of agar and 0.1g of chloramphenicol, and the pH of the medium is 6.0±0.2.
[0028] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (availa...
Embodiment 2
[0075] The Inhibitory Effect of the Metabolites of the Strain on the Pathogen of Panax notoginseng Anthracnose
[0076] 1. Fermentation culture of bacteria and extraction of fermentation products
[0077] (1) Inoculate the endophytic fungus TRXY-34-1 of Sophora vietnamese on a flat plate of potato dextrose agar medium (PDA plate), culture it at 28°C for 20 days, cut the obtained culture material into pieces and transfer to In the Erlenmeyer flask of 2 liters of aseptic solid culture medium (containing the dextrose of 400g potato, 20g and 20g sucrose), place 28 ℃ of conditions to ferment for 60 days;
[0078] (2) After the fermentation is completed, soak the fermented product with 2 times the amount of methanol of the fermented product and sonicate it for 40 minutes, then filter it with gauze, and get the filtrate;
[0079] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract, and obtain the methanol crude extract of th...
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