Preparation method of effective polypeptide components in Vespula insects, and medicinal uses of effective polypeptide components

A technology of effective parts and vespa, applied in the field of medicine, can solve problems such as failure to form large-scale production capacity and low level of industrial production support, and achieve the effects of reducing animal brain tissue permeability, reducing cerebral edema, and reducing cerebral embolism.

Inactive Publication Date: 2014-03-26
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Preparation method of effective polypeptide components in Vespula insects, and medicinal uses of effective polypeptide components
  • Preparation method of effective polypeptide components in Vespula insects, and medicinal uses of effective polypeptide components
  • Preparation method of effective polypeptide components in Vespula insects, and medicinal uses of effective polypeptide components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 : Preparation of active parts of Vespa black shield bee venom polypeptide and film-coating agent

[0034] 1.1 Collection of crude venom from Vespa black shield:

[0035] The black-shield wasp was collected from Simao, Yunnan Province. The bee body specimen was identified as Vespa bicolor bicolor Fabricius (black-shield wasp) by Professor Mao Benyong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 12V , output automatic intermittent pulsating voltage; method of collecting wasp venom: place the poison collecting device in front of the hive, and collect poison for 30 minutes according to the routine poison collecting procedure on t...

Embodiment 2

[0040] Example 2 : Preparation of effective parts of golden hornet bee venom polypeptide and hydrogel, cataplasm and coating agent

[0041] 2.1 Collection of crude venom of golden hornet:

[0042] The golden hornet was collected from Lianghe County, Yunnan Province. The bee specimen was identified as Vespa mandarinia Smith (also known as Golden Ring Vespa) by Professor Guo Yunjiao of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 12V , output automatic intermittent pulsating voltage; method of collecting wasp venom: put the unpainted poison collector in front of the hive, and collect poison for 30 minutes according to the routine poison collectio...

Embodiment 3

[0051] Example 3 : Preparation of coating agent for active part of Vespa dentata venom polypeptide

[0052] 3.1 Collection of crude venom from Vespa dentata:

[0053] Vespa auralia was collected from Yunlong County, Dali Prefecture, Yunnan Province. The bee body specimen was identified as Vespa auralia Smith by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 12V, Output automatic intermittent pulsating voltage; The wooden frame is painted red with red paint in advance; The wooden frame is pre-smeared with 20 milliliters of the honeycomb and honeycomb extract volatile solution (preparation m...

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Abstract

The invention relates to a preparation method of effective polypeptide components in Vespula insects, and medicinal uses of the effective polypeptide components. The effective components are obtained through a step of dialyzing crude hornet venom through a 30KDa dialysis membrane, and a step of freeze-drying or reduced pressure concentration drying of the above obtained dialysis liquid. A series of pharmacodynamic experiments verify that the effective biotoxin polypeptide components and transdermal absorption preparations thereof can substantially control the cerebral thrombus, can recover the mobility of cerebral palsy animals, can mitigate the ischemic brain injury, have stronger pharmacodynamic performances than first-line medicines comprising plavix, Xingnaojing and the like, and can be expected to be used for preparing innovative biochemical medicines for preventing and treating cardiovascular and cerebrovascular diseases in order to prevent and treat ischemic cardiocardiovascular diseases, such as myocardial infarction, cardiovascular thrombus, cerebral thrombus, cerebral infarction, acute / chronic cerebral apoplexy, and sequelae caused by cerebral infarction injury, like lateral hemiplegia, hemidysesthesia, hemiopia, facial and lingual paralysis, aphasia and general paralysis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to a preparation method and medical use of effective parts of polypeptides in insects of the genus Vespa. The effective part is obtained by dialysis of crude bee venom of Vespa vespa with a 25KDa dialysis membrane, and the eluate is freeze-dried or concentrated and dried under reduced pressure. Through a series of pharmacological and pharmacodynamic experiments in animals, the inventors found that the effective part of the polypeptide has the effect of significantly preventing and treating cerebral thrombosis, restoring the activity ability of cerebral palsy animals, and reducing ischemic brain damage, and can be expected to be used in the preparation of anti-cardiac and cerebrovascular diseases. Vascular diseases include drugs, health care products, medical devices and daily chemicals for ischemic cerebrovascular disease and ischemic cardiovascular disease. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34A61K38/17A61P9/10A61P7/02A61P9/00A61P25/00
CPCA61K38/00C07K14/43572
Inventor 杨毅梅高孟婷王斌巫秀美李成功赵昱张成桂
Owner DALI UNIV
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