White kidney bean extract extracted by microwave as well as preparation method and application for same

Abstract

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U/mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w/w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of white kidney bean extract, in particular to a microwave-extracted white kidney bean extract and its preparation method and application, belonging to the technical field of biology. Background technique [0002] White kidney bean, whose biological name is multi-flowered bean, is named for its variety of flowers and colors. Belongs to Fabaceae, Papilionaceae, Bean family Bean genus, scientific name is phaseolus coccineus L., P.multiflorus willd. English name is Scarlet runner bean, Multiflora bean, white kidney bean. White kidney beans are native to the tropical regions of South America, and have been introduced and cultivated on a large scale in Yunnan and other places in my country. White kidney beans are very nutritious. The protein content is higher than that of chicken, the calcium content is more than 7 times that of chicken, the iron is 4 times, and the B vitamins are also higher than chicken. At ...

AI Technical Summary

Problems solved by technology

The patent "A Method for Extracting α-Amylase Inhibitors from Kidney Beans" (CN101269111A) discloses a method of ultrasonically extracting buffer solution, followed by steps such as filtration, heating, centrifugation, dialysis, alcohol precipitation, and drying to obtain high-content α-amylase inhibitors. -The method of amylase inhibitor, the shortcoming of this method is that production cycle is long, and process link is many, and the protease inhibitor product yield and activity obtained by heating time are long are all lower

The patent "Using ethanol-precipitated kidney bean extract, its application and preparation" (CN101340924A) discloses a method for extracting water and ethanol at different concentrations with a buffer to precipitate impurities and amylase inhibitors respectively. The disadvantage of this method is that the process is not Good control, the content and activity of the product are not high

[0005] Most of the α-amylase inhibitors reported in the literature are traditional heating extraction, organic solvent precipitation or inorganic salt precipitation and other methods. These methods have long production cycle, high energy consumption, high pollution, low product quality, high cost, Disadvantages such as many chemical residues

At present, the high content of α-amylase inhibitor products in white kidney bean products in the industry is high in price, without taking into account other nutritional components. The low content of white kidney bean extracts currently on the market contain only about 1% phaseolin, and most of them are concentrated and dried through simple extraction. Prepared by the same method, the biological activity is poor, the dosage is large, and the health care effect is not obvious

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