White kidney bean extract extracted by microwave as well as preparation method and application for same

A white kidney bean extract and extract technology, applied in the field of biology, can solve the problems of low product content and activity, poor biological activity, and many chemical residues, achieve good application prospects and economic value, mild extraction conditions, avoid Effects of Environmental Pollution

A white kidney bean extract and extract technology, applied in the field of biology, can solve the problems of low product content and activity, poor biological activity, and many chemical residues, achieve good application prospects and economic value, mild extraction conditions, avoid Effects of Environmental Pollution

CN102552376AInactive Publication Date: 2012-07-11西安天一生物技术股份有限公司
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  • White kidney bean extract extracted by microwave as well as preparation method and application for same
  • White kidney bean extract extracted by microwave as well as preparation method and application for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Grind 100kg of white kidney beans with a pulverizer, pass through a 60-mesh sieve, mix them evenly with pure water at a weight ratio of 1:5, place them in a microwave extraction device for extraction twice below 40°C, power: 1000W, frequency: 2450MHz, Each time for 3 minutes, filter, combine the filtrates, and discard the filter residue. Heat the filtrate in a stirring water bath to 70°C for 20 minutes, pass through a Φ50×600cm chromatography column filled with 200L silica gel at 30°C, collect the effluent, and then rinse with 200L of pure water to collect the effluent. The effluents were combined and vacuum-dried below 50° C. to obtain 2.03 kg of white kidney bean extract, the phaseolin activity was 4500 U / mg, and the HPLC content: 23.2% W / W. HPLC detection conditions are:

[0037] Instrument: SHIMADZU 20A series high performance liquid chromatography; column type: TSKgel, Octadecyl-2PW fully porous hydrophobic resin (5μm, pore size 20nm), φ4.6×150mm; mobile phase: A ...

Embodiment 2

[0039]Grind 100kg of white kidney beans with a pulverizer, pass through a 60-mesh sieve, mix them evenly with pure water at a weight ratio of 1:5, and place them in a microwave extraction device for extraction twice below 40°C, power: 2000W, frequency: 2450MHz per Once for 1 minute, filter, combine the filtrates, and discard the filter residue. Heat the filtrate in a stirring water bath to 70°C for 20 minutes, pass through a Φ50×600cm chromatography column filled with 200L silica gel at 30°C, collect the effluent, and then rinse with 200L of pure water to collect the effluent. The effluents were combined and freeze-dried at low temperature to obtain 1.82 kg of white kidney bean extract, the phaseolin activity was 4100 U / mg, and the HPLC content: 20.4% W / W.

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Abstract

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U / mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w / w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

Description

technical field [0001] The invention relates to a preparation method of white kidney bean extract, in particular to a microwave-extracted white kidney bean extract and its preparation method and application, belonging to the technical field of biology. Background technique [0002] White kidney bean, whose biological name is multi-flowered bean, is named for its variety of flowers and colors. Belongs to Fabaceae, Papilionaceae, Bean family Bean genus, scientific name is phaseolus coccineus L., P.multiflorus willd. English name is Scarlet runner bean, Multiflora bean, white kidney bean. White kidney beans are native to the tropical regions of South America, and have been introduced and cultivated on a large scale in Yunnan and other places in my country. White kidney beans are very nutritious. The protein content is higher than that of chicken, the calcium content is more than 7 times that of chicken, the iron is 4 times, and the B vitamins are also higher than chicken. At ...

Claims

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Application Information

Patent Timeline
11 Jul 2012
Publication
CN102552376A
IPC
A61K36/48; A61P3/04
Inventors
叶涛; 李凡