Mixture of momordica saponins and polysaccharides and extraction process and application thereof

Abstract

The invention discloses a mixture of momordica saponins and polysaccharides, which is obtained by the following preparation methods: the balsam pear is used as a raw material and is added with an organic solvent to undergo ultrasound-microwave assisted extraction; a momordica saponins extract is obtained after filtration; momordica filter residues are extracted in alkaline solution; the obtained extract is namely the momordica polysaccharides extract; the momordica saponins extract and the momordica polysaccharides extract are respectively concentrated, dried and mixed to obtain a mixture of momordica polysaccharides and saponins; the invention also discloses an extraction process and application of the mixture of the momordica saponins and polysaccharides; the mixture has various physiological functions such as blood sugar level reduction, cancer resistance, virus resistance, blood lipids regulation, immunity increasing and the like; the mixture can be used for preparing health food with the function of reducing blood sugar level; the extraction process adopts step-by-step extraction technology and has the characteristics of high raw materials utilization rate, mild extraction conditions, high speed, high efficiency and the like; compared with the single extraction technology, for the extraction process of the invention, the extraction time is shortened by more than 20% and the purity of the product is improved by more than 15%.

Description

technical field

[0001] The invention belongs to the field of agricultural product processing, and in particular relates to a mixture of bitter melon saponin and polysaccharide and its extraction process and application. Background technique

[0002] Bitter gourd has been used in my country for nearly 500 years, and is a famous folk medicine and commonly used vegetable. It has been used clinically in the past dynasties to treat tumors, dysentery, heatstroke and other diseases. "Compendium of Materia Medica" records: bitter gourd "bitter cold, non-toxic, eliminating evil heat, relieving fatigue, clearing heart and eyesight, nourishing qi and strengthening yang". A large number of modern studies have shown that bitter melon is rich in polysaccharides and saponins, which have various physiological functions such as lowering blood sugar, anti-cancer, anti-virus, regulating blood lipids, and enhancing immunity. Therefore, it is of great development and application value to study...

Examples

Embodiment 1

[0031] Take 1 kg of dried bitter melon, grind it to 250 mesh, add 18 kg of ethanol solution with a volume concentration of 75%, soak it for 1 hour, and then extract it with 60w, 20khz ultrasonic-microwave for 800s; filter the above extract, and the filtrate is bitter melon Saponin extract; for the filter residue obtained by the above filtration, add an aqueous NaOH solution with a weight ratio of material to liquid of 1:35 and a pH of 9 to extract at 80°C for 25min, then filter, and the aqueous solution obtained by filtration is the bitter gourd polysaccharide extract; The bitter melon saponin extract and the bitter gourd polysaccharide extract are concentrated to 1 / 7.5 of the original volume to obtain the bitter gourd saponin concentrate and the bitter gourd polysaccharide concentrate; respectively freeze-dry the bitter gourd saponin concentrate and the bitter gourd polysaccharide concentrate, and press 1:1 The mixture of bitter gourd polysaccharide and saponin can be obtained...

Embodiment 2

[0033] Take 1 kg of dried bitter melon, grind it into 300 meshes, add 12 kg of ethanol solution with a volume concentration of 65%, soak for 1 hour, and then use 50w, 25khz ultrasonic-microwave synergistic extraction for 1150s; filter the above extract, and the filtrate is bitter melon Saponin extract; for the filter residue obtained by the above filtration, add a NaOH aqueous solution with a weight ratio of material to liquid of 1:40 and a pH of 8 to extract at 85°C for 20 minutes, then filter, and the aqueous solution obtained by filtration is the bitter gourd polysaccharide extract; The bitter melon saponin extract and the bitter melon polysaccharide extract are concentrated to 1 / 5 of the original volume to obtain the bitter melon saponin concentrate and the bitter gourd polysaccharide concentrate; respectively freeze-dry the bitter melon saponin concentrate and the bitter gourd polysaccharide concentrate, and press 1:1 The mixture of bitter gourd polysaccharide and saponin ...

Embodiment 3

[0035] Take 1 kg of dried bitter gourd, ultrafinely grind it to 350 mesh, add 24 kg of ethanol solution with a volume fraction concentration of 85%, soak for 1 hour, and then extract 975 seconds under 40w, 15khz ultrasonic-microwave synergy; filter the above extract, and the filtrate is Momordica saponin extract; for the filter residue obtained by the above filtration, add a NaOH solution with a weight ratio of solid to liquid of 1:30 and a pH of 7 to extract at 90°C for 30 minutes, then filter, and the aqueous solution obtained by filtering is the bitter gourd polysaccharide extract; The obtained balsam pear saponin extract and balsam pear polysaccharide extract are concentrated to 1 / 10 of the original volume to obtain balsam pear saponin concentrate and balsam pear polysaccharide concentrate; respectively freeze-dry the balsam pear saponin concentrate and balsam pear polysaccharide concentrate, according to 1: After mixing at a weight ratio of 1, the bitter gourd polysacchari...

technical field

[0001] The invention belongs to the field of agricultural product processing, and in particular relates to a mixture of bitter melon saponin and polysaccharide and its extraction process and application. Background technique

[0002] Bitter gourd has been used in my country for nearly 500 years, and is a famous folk medicine and commonly used vegetable. It has been used clinically in the past dynasties to treat tumors, dysentery, heatstroke and other diseases. "Compendium of Materia Medica" records: bitter gourd "bitter cold, non-toxic, eliminating evil heat, relieving fatigue, clearing heart and eyesight, nourishing qi and strengthening yang". A large number of modern studies have shown that bitter melon is rich in polysaccharides and saponins, which have various physiological functions such as lowering blood sugar, anti-cancer, anti-virus, regulating blood lipids, and enhancing immunity. Therefore, it is of great development and application value to study...

Examples

Embodiment 1

[0031] Take 1 kg of dried bitter melon, grind it to 250 mesh, add 18 kg of ethanol solution with a volume concentration of 75%, soak it for 1 hour, and then extract it with 60w, 20khz ultrasonic-microwave for 800s; filter the above extract, and the filtrate is bitter melon Saponin extract; for the filter residue obtained by the above filtration, add an aqueous NaOH solution with a weight ratio of material to liquid of 1:35 and a pH of 9 to extract at 80°C for 25min, then filter, and the aqueous solution obtained by filtration is the bitter gourd polysaccharide extract; The bitter melon saponin extract and the bitter gourd polysaccharide extract are concentrated to 1 / 7.5 of the original volume to obtain the bitter gourd saponin concentrate and the bitter gourd polysaccharide concentrate; respectively freeze-dry the bitter gourd saponin concentrate and the bitter gourd polysaccharide concentrate, and press 1:1 The mixture of bitter gourd polysaccharide and saponin can be obtained...

Embodiment 2

[0033] Take 1 kg of dried bitter melon, grind it into 300 meshes, add 12 kg of ethanol solution with a volume concentration of 65%, soak for 1 hour, and then use 50w, 25khz ultrasonic-microwave synergistic extraction for 1150s; filter the above extract, and the filtrate is bitter melon Saponin extract; for the filter residue obtained by the above filtration, add a NaOH aqueous solution with a weight ratio of material to liquid of 1:40 and a pH of 8 to extract at 85°C for 20 minutes, then filter, and the aqueous solution obtained by filtration is the bitter gourd polysaccharide extract; The bitter melon saponin extract and the bitter melon polysaccharide extract are concentrated to 1 / 5 of the original volume to obtain the bitter melon saponin concentrate and the bitter gourd polysaccharide concentrate; respectively freeze-dry the bitter melon saponin concentrate and the bitter gourd polysaccharide concentrate, and press 1:1 The mixture of bitter gourd polysaccharide and saponin ...

Embodiment 3

[0035] Take 1 kg of dried bitter gourd, ultrafinely grind it to 350 mesh, add 24 kg of ethanol solution with a volume fraction concentration of 85%, soak for 1 hour, and then extract 975 seconds under 40w, 15khz ultrasonic-microwave synergy; filter the above extract, and the filtrate is Momordica saponin extract; for the filter residue obtained by the above filtration, add a NaOH solution with a weight ratio of solid to liquid of 1:30 and a pH of 7 to extract at 90°C for 30 minutes, then filter, and the aqueous solution obtained by filtering is the bitter gourd polysaccharide extract; The obtained balsam pear saponin extract and balsam pear polysaccharide extract are concentrated to 1 / 10 of the original volume to obtain balsam pear saponin concentrate and balsam pear polysaccharide concentrate; respectively freeze-dry the balsam pear saponin concentrate and balsam pear polysaccharide concentrate, according to 1: After mixing at a weight ratio of 1, the bitter gourd polysacchari...

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