Method for extracting dietary fibers from dendrobium officinale
A technology of dietary fiber and Dendrobium candidum, applied in food preparation, food science, application, etc., can solve the problems of raw material waste of Dendrobium candidum, improve the effective extraction efficiency and comprehensive utilization rate, avoid resource waste, and achieve high extraction efficiency Effect
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Embodiment 1
[0057] This example is a preparation example of extracting dietary fiber from defatted dendrobium powder, omitting the material processing steps.
[0058] The first step: preparing the dietary fiber crude extract, which specifically includes the following steps:
[0059] Step 1.1: Flash extraction.
[0060] First, take 18 g of defatted Dendrobium powder made from 57.0 g of fresh Dendrobium officinale stems and leaves (water content 80%), add 50 times 85% ethanol, and adjust the pH to 10. Ethanol is added gradually, constantly stirring, so that the defatted dendrobium powder is evenly distributed in it, and avoids agglomeration. After mixing, adjust the pH to 10, and extract with a flash extractor for 2 minutes, divided into two times, 30 seconds each time, and adjust the flash extractor to 120V. Then centrifuge, the condition of centrifugation is 5000r / min, 15min. Precipitate A was obtained after centrifugation.
[0061] Step 1.2: Enzymatic digestion.
[0062] Take the prec...
Embodiment 2
[0083] This example is another preparation example of extracting dietary fiber from defatted dendrobium powder, which omits the material processing steps.
[0084] The first step: preparing the dietary fiber crude extract, which specifically includes the following steps:
[0085] Step 1.1: Flash extraction.
[0086] First, take 15 g of defatted Dendrobium powder made from 60.2 g of fresh stems and leaves of Dendrobium officinale, add 50 times of 85% ethanol, and adjust the pH to 10. Ethanol is added gradually, constantly stirring, so that the defatted dendrobium powder is evenly distributed in it, and avoids agglomeration. After mixing, adjust the pH to 10, and extract with a flash extractor for 1 minute, divided into two times, 60 seconds each time, and adjust the flash extractor to 120V. Then centrifuge, the condition of centrifugation is 5000r / min, 30min. Precipitate A was obtained after centrifugation.
[0087] Step 1.2: Enzymatic digestion.
[0088] Take the precipit...
Embodiment 3
[0109] This example is the preparation example of extracting dietary fiber from fresh Dendrobium officinale.
[0110] The first step: material pretreatment:
[0111] Weigh 69.1g of fresh Dendrobium officinale stems and leaves frozen at -20°C, cut into pieces, dry, and pulverize. Then use sherwood oil to reflux for 4 hours, filter it, and then use sherwood oil to wash the dendrobium powder, so as to obtain the defatted dendrobium powder, which is reserved for use;
[0112] The second step: prepare the dietary fiber crude extract, which specifically includes the following steps:
[0113] Step 2.1: Flash extraction.
[0114] First, take 20 g of defatted dendrobium powder, add 50 times of 85% ethanol, and adjust the pH to 10. Ethanol is added gradually, constantly stirring, so that the defatted dendrobium powder is evenly distributed in it, and avoids agglomeration. After mixing, adjust the pH to 10, and extract with a flash extractor for 2 minutes, divided into two times, 30 ...
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