Method for extracting flavonoid glycoside compounds in herba epimedii
A technology of flavonoid glycosides and flavonoid glycosides in Huozhong, which is applied in the field of traditional Chinese medicine extraction, can solve the problems of flammability and explosion, and achieve the effect of simple operation, mild extraction conditions and low loss
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] Prepare the deep eutectic solvent of choline chloride and phenol:
[0039] Choline chloride and phenol with a molar ratio of 1:2 were stirred under anhydrous conditions, heated at 60°C until a uniform and stable transparent solution was formed, and sealed for storage.
[0040] In the invention, the deep eutectic solvent is used for the treatment of flavonoid glycoside standard products, and the flavonoid glycoside substances are sensitively detected by means of high-performance liquid chromatography. Specific steps are as follows:
[0041] 1) Weigh a certain amount of labeled products of four flavonoid glycosides Epimedin A, Epimedin B, Epimedin C, and Icariin, and add methanol to form Epimedin A, Epimedin B, Epimedin B, and Epimedin B. A mixed solution of flavonoid glycosides with epimedin C and icariin concentrations of 0.040, 0.039, 0.171, and 0.273 mg / ml, respectively;
[0042] 2) Measure 2.0ml of the mixed solution obtained in step (1), add 4.0ml of deep eutectic...
Embodiment 2
[0049] A method utilizing a deep eutectic solvent to extract flavonoid glycosides in Epimedium leaves, comprising the following steps:
[0050] 1) According to the method described in Example 1, a deep eutectic solvent of choline chloride-phenol (1:2) was prepared.
[0051] 2) Grind dried Epimedium, pass through a 80-mesh sieve, take 0.05 grams of Epimedium powder, add 2ml of choline-like deep eutectic solvent, extract by ultrasonic at 40°C for 30 minutes, power 100W / cm 2 , add 2 ml of water to dilute, and vortex for 2 minutes to obtain flavonoid glycosides extract;
[0052] 3) Add water to the flavonoid glycoside compound extract obtained in step 2) to make up to 10 ml, filter through a 0.45 μm filter membrane to remove impurities, and obtain a filtered extract.
[0053] Inject 5 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions under the item of Example 1 for detection. Chromatogram see figure 2. The ...
Embodiment 3
[0055] A method utilizing a deep eutectic solvent to extract flavonoid glycosides in Epimedium leaves, comprising the following steps:
[0056] 1) According to the method described in Example 1, a deep eutectic solvent of choline chloride-1,2-propanediol (1:2) was prepared.
[0057] 2) Take 0.05 g of Epimedium powder that has been crushed and passed through a 40-mesh sieve, add 2 ml of a choline-based deep eutectic solvent, and conduct ultrasonic extraction at 50°C for 40 minutes with a power of 100W / cm 2 , add 2 ml of water to dilute, and vortex for 2 minutes to obtain flavonoid glycosides extract;
[0058] 3) Add water to the flavonoid glycoside compound extract obtained in step 2) to make up to 10 ml, filter through a 0.45 μm filter membrane to remove impurities, and obtain a filtered extract.
[0059] The obtained extract was detected by injecting 5 μL of samples into a high-performance liquid chromatograph, and the yields of epimedin A, epimedin B, epimedin C and icariin...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap