Technology for preparing functional dietary fiber through myrica ruba marc enzymolysis
A technology of dietary fiber and bayberry pomace, which is applied in the directions of food preparation, food science, application, etc., achieves the effects of low production cost, good quality and alleviating hunger.
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Embodiment 1
[0014] Red bayberry pomace preparation: The bayberry pomace after squeezing the bayberry juice is directly used for later use.
[0015] Organic solvent extraction: take by weighing the pomace after the red bayberry juice that folds dry powder 250g (red bayberry pomace after squeezing the juice is through 60~80 ℃ of temperature drying pulverizations, the dry powder weight after crossing 80~100 mesh sieves), Put it in a closed container and seal and extract with ether to remove the lipids in the bayberry pomace. The amount of ether added is based on submerging the pomace. After leaching for 24 hours, the organic solvent is recovered by pressure filtration, the red bayberry pomace is dried, the residual organic solvent in the red bayberry pomace is removed, and ether is recovered. Here is the weight of dry powder
[0016] Cellulose enzymatic hydrolysis: add 20 mL / g of phosphate buffer solution (red bayberry pomace dry matter weight) of pH=4.5 to the degreased and dried red baybe...
Embodiment 2
[0025] Red bayberry pomace was recovered, dried at 60°C, crushed, and passed through a 80-mesh sieve.
[0026] Organic solvent leaching: Weigh 300g of dry powder, put it in a closed container, and seal it with ether for extraction to remove the lipids in the bayberry pomace. The amount of ether added is based on submerging the pomace. After leaching for 25 hours, the organic solvent is recovered by pressure filtration, the red bayberry pomace is dried, the residual organic solvent in the red bayberry pomace is removed, and ether is recovered.
[0027] Cellulose enzymatic hydrolysis: add 20mL / g of phosphate buffer solution (red bayberry pomace dry matter weight) of pH=5.5 to the degreased and dried red bayberry pomace, the enzyme concentration is 0.3%, and the enzyme activity is 6u / mL cellulose Enzyme solution, at 120rpm, in a 55°C water bath, digest for 1 hour, then raise the temperature to 100°C, and keep for 5 minutes to inactivate the enzyme.
[0028] Alcohol analysis: The...
Embodiment 3
[0036] Red bayberry pomace was recovered, dried at 60°C, crushed, and passed through a 80-mesh sieve.
[0037] Organic solvent extraction: Weigh 500g of dry powder, put it in a closed container, seal and extract with ether, to remove the lipids in the bayberry pomace, the amount of ether added is based on submerging the pomace. After leaching for 23 hours, press filtration to recover the organic solvent, dry the red bayberry pomace, remove the residual organic solvent in the red bayberry pomace, and recover ether.
[0038] Cellulose enzymatic hydrolysis: add 20mL / g of phosphate buffer solution (20mL / g of red bayberry pomace dry matter weight) of pH=5 to the degreased and dried red bayberry pomace, the enzyme concentration is 0.5%, and the enzyme activity is 8u / mL cellulose Enzyme solution, at 120rpm, in a 60°C water bath, for 2 hours, then raise the temperature to 100°C, and keep for 5 minutes to inactivate the enzyme.
[0039] Alcohol analysis: The degraded enzymolysis solut...
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