Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer

A technology of Bacillus saccharolyticus and compound microorganisms, which is applied to the strains of Bacillus coldolyticus and its application in compound microbial seaweed fertilizers, can solve the problems of decreased disease resistance of crops, decreased land quality, and low utilization rate of fertilizers, etc. Remarkable ability to resist diseases and insect pests and promote growth, strong ability to degrade cellulose and starch, enhance stress resistance and biological activity

Active Publication Date: 2011-09-07
青岛海大生物集团股份有限公司
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Problems solved by technology

The use of a large number of chemical fertilizers and pesticides has solved the problem of food supply and increased economic income on the one hand

Method used

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  • Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer

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Embodiment Construction

[0013] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0014] (1) Isolation and screening of Bacillus cold-lyzed

[0015] Take 50-100 samples from naturally fermented noodle sauce, compost, fruit tree soil, and vegetable garden soil, take 1-10g samples respectively, suspend in 9-100ml physiological saline, and shake vigorously. Take 100 μL of sample suspension and evenly coat the plate. In the embodiment of the present invention, ordinary nutrient broth medium is used as the sterile nutrient solution, calculated in grams per liter, and its ingredients are peptone 10.0, beef powder 3.0, sodium chloride 5.0, Glucose 1.0, water 1000 ml, pH value 7.5, sterilized at 121°C for 20 minutes, incubation temperature at 37°C, incubation time 18-24 hours. Plate colony counting medium is nutrient agar medium, that is, agar is added to common nutrient broth medium, the culture temperature is 37°C, and the...

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Abstract

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion/g to 1 billion/g and an organic matter content of 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

Description

technical field [0001] The invention relates to a strain of Bacillus pyrolyticum and its application in compound microbial seaweed fertilizer. Background technique [0002] Soil-borne diseases appearing in various parts of our country are becoming more and more serious and are on the rise. Fusarium wilt, nematode disease, blight and viral diseases are spreading. The use of a large number of chemical fertilizers and pesticides, on the one hand, has solved the problem of food supply and increased economic income; but on the other hand, chemical pesticides have also brought about more serious negative effects: low fertilizer utilization rate, environmental degradation, land quality decline, Water pollution and the decline of crop disease resistance have largely threatened food security and the sustainable development of agriculture. Microbial fertilizer has its unique function in solving this problem and has achieved good results. It will be the focus and support of the develo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08A01P1/00A01P3/00A01P5/00C12R1/07
Inventor 宋海妹单俊伟乔来艳李秀珍王海华
Owner 青岛海大生物集团股份有限公司
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