Bacillus psychrosaccharolyticus strain and application thereof in complex microorganism seaweed fertilizer
A technology of Bacillus saccharolyticus and compound microorganisms, which is applied to the strains of Bacillus coldolyticus and its application in compound microbial seaweed fertilizers, can solve the problems of decreased disease resistance of crops, decreased land quality, and low utilization rate of fertilizers, etc. Remarkable ability to resist diseases and insect pests and promote growth, strong ability to degrade cellulose and starch, enhance stress resistance and biological activity
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[0013] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0014] (1) Isolation and screening of Bacillus cold-lyzed
[0015] Take 50-100 samples from naturally fermented noodle sauce, compost, fruit tree soil, and vegetable garden soil, take 1-10g samples respectively, suspend in 9-100ml physiological saline, and shake vigorously. Take 100 μL of sample suspension and evenly coat the plate. In the embodiment of the present invention, ordinary nutrient broth medium is used as the sterile nutrient solution, calculated in grams per liter, and its ingredients are peptone 10.0, beef powder 3.0, sodium chloride 5.0, Glucose 1.0, water 1000 ml, pH value 7.5, sterilized at 121°C for 20 minutes, incubation temperature at 37°C, incubation time 18-24 hours. Plate colony counting medium is nutrient agar medium, that is, agar is added to common nutrient broth medium, the culture temperature is 37°C, and the...
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