Method for extracting skunk bush polysaccharide by zymohydrolysis

A technology of Cornus officinalis polysaccharides and Cornus officinalis, applied in the direction of fermentation, etc., can solve the problems of difficult wide application, long time, high energy consumption, etc., and achieve the effect of easy removal of impurities, maintenance of polysaccharide activity, and mild conditions

Inactive Publication Date: 2009-01-21
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hot water extraction takes a long time, consumes a lot of energy, has high impurity content, and low yield; although acid or alkali extraction can increase the extraction rate, it is easy to destroy the structure of polysaccharides
In addition, acid and alkali leaching have a certain impact on the equipment, and need to undergo neutralization reaction, the operation procedures are relatively cumbersome, and it is difficult to be widely used in industrial production
At present, there is no report on the extraction of polysaccharides from Cornus officinalis by enzymatic treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Drying and crushing: take the dried dried pulp of Cornus officinalis, grind and crush it into dry powder of Cornus officinalis of 30 meshes.

[0018] (2) Enzyme treatment: Take 10.0g of Cornus officinalis dry powder, add 150g of distilled water to mix, add 0.6g of papain (enzyme activity 800000U / g, Guangbo Nanning Pangbo Bioengineering Co., Ltd.), and use 1.0mol / LNaOH and 1.0mol / L HCl Adjust the pH of the solution to 6.0. Enzymolysis extraction was carried out at a temperature of 45°C for 1.5 hours.

[0019] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 100°C to inactivate the enzyme for 0.5h.

[0020] (4) Alcohol precipitation: After the extract is centrifuged, the supernatant is concentrated to 1 / 6 of the original volume, 2 times the volume of 100% ethanol is added to the concentrated solution, stirred evenly and then centrifuged for 12 hours, and the precipitate is freeze-dried to obtain Cornus officinalis polysaccharide 0....

Embodiment 2

[0022] (1) Drying and crushing: take the dried dried pulp of Cornus officinalis, grind and crush it into dry powder of Cornus officinalis of 30 meshes.

[0023] (2) Enzyme treatment: take 10.0g of cornus officinalis dry powder, add 150g of distilled water to mix, then add pectinase (enzyme activity 20000U / g, Tianjin Lihua Enzyme Preparation Factory) 0.6g, papain (enzyme activity 800000U / g, Guangxi Nanning Pangbo Bioengineering Co., Ltd.) 0.6g, with 1.0mol / L NaOH and 1.0mol / L HCl to adjust the pH value of the solution to 5.0. Enzymolysis extraction was carried out at a temperature of 35°C for 2.0 hours.

[0024] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 100°C to inactivate the enzyme for 0.5h.

[0025] (4) Alcohol precipitation: After the extract is centrifuged, the supernatant is concentrated to 1 / 2 of the original volume, 5 times the volume of 70% ethanol is added to the concentrated solution, stirred evenly and then centrifuged for ...

Embodiment 3

[0027] (1) Drying and crushing: take the dried dried fruit of Cornus officinalis, grind and crush it into dry powder of Cornus officinalis of 100 meshes.

[0028] (2) Enzyme treatment: take 10.0g of cornus officinalis dry powder, add 300g of distilled water to mix, then add pectinase (enzyme activity 20000U / g, Tianjin Lihua Enzyme Preparation Factory) 0.3g, papain (enzyme activity 800000U / g, Guangxi Nanning Pangbo Biological Engineering Co., Ltd.) 0.3g, cellulase (enzyme activity 15000U / g, Wuxi Xuemei Enzyme Technology Co., Ltd.) 0.3g, starch hydrolase (enzyme activity 2000U / g, Beijing Donghua Qiangsheng Biotechnology Co., Ltd.) 0.3g, adjust the pH value of the solution to 7.0 with 1.0mol / LNaOH and 1.0mol / LHCl. Enzymolysis extraction was carried out at a temperature of 60°C for 0.5h.

[0029] (3) Enzyme inactivation at high temperature: After enzymatic hydrolysis, heat up to 90°C to inactivate the enzyme for 1.5 hours.

[0030] (4) Alcohol precipitation: After the extract is...

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PUM

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Abstract

The invention provides a method for extracting cornel polysaccharide through enzymolysis. The method comprises the following steps that cornel dry flesh is crushed to make cornel dry powder; distilled water is added with the mass being 15 to 60 times than that of the cornel dry powder, and an enzyme are added so as to carry out enzymolysis at a temperature of between 35 and 60 DEG C and a pH value of between 3.5 and 7.0; after the enzymolysis is finished, enzyme inactivation is carried out; an extraction solution is separated and purified so as to obtain a cornel polysaccharide extract; the enzyme is a mixture comprising one sort or more than two sorts of (1) cellulase, (2) papain, (3) pectinase and (4) amylolytic enzyme; and the dose by mass of the enzymes accounts for 0.1 to 24 percent of the mass of the cornel dry powder. The method has the advantages that compared with the prior hot water extraction method, the method can increase the extraction rate of cornel polysaccharide by 10 to 50 percent and the content of cornel polysaccharide by 15 to 80 percent; and due to preventing the influence of the prior hot water extraction, acid extraction or alkaline extraction on the structure of polysaccharide, and adopting enzymolysis treatment to extract cornel polysaccharide, the method has the advantages of moderate conditions, easy removal of impurities, furthest maintained activity of polysaccharide, and the like.

Description

(1) Technical field [0001] The invention relates to a method for extracting cornus officinalis polysaccharide by enzymolysis. (2) Background technology [0002] The traditional Chinese medicine Cornus officinalis is the dried pulp of Cornus officinalis Sieb et Zu cc. It tastes sour, astringent, and slightly warm, and it returns to the liver and kidney meridian. material. Clinical studies of traditional Chinese medicine have proved that Cornus officinalis is the main medicinal material for treating diabetes, coronary heart disease and hypertension. [0003] At present, the research on Cornus officinalis at home and abroad mainly focuses on the chemical components and pharmacological effects. Its main components are iridoid glycosides, pentacyclic triterpene acids and their esters, tannins, volatile oils and polysaccharides, etc. It has pharmacological activities such as immune regulation, lowering blood sugar, anti-shock, anti-arrhythmia, anti-bacterial, anti-inflammatory, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 程俊文吴学谦贺亮付立忠魏海龙吴庆其李海波
Owner ZHEJIANG FORESTRY ACAD
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