Method for extracting alpha-diastase restrainer from phaseolus limensis

A technology of amylase inhibitor and kidney bean, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, peptide sources, etc., can solve the problems of reducing the biological activity of the product, increasing the viscosity of the solution, and large solvent consumption, and achieves good application. Prospects and economic value, the effect of reducing the pressure of purification, reducing production costs

Inactive Publication Date: 2008-09-24
SHAANXI ZHUORUI BIOTECH
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AI Technical Summary

Problems solved by technology

Heating during the extraction process will easily lead to gelatinization of part of the starch and increase the viscosity of the solution, which will bring difficulties to the subsequent filtration and centrifugation operations, and long-term heating and extraction will reduce the biological activity of the product; due to the limitation of the process, raw materials Can not be crushed too finely, resulting in reduced fragmentation of the cell wall, which is not conducive to the complete release of amylase inhibitors in the cells, and requires a large amount of extraction solvent and long-term extraction
Therefore, the traditional heating solvent extraction method has the disadvantages of high energy consumption, large solvent consumption, long production cycle, high environmental protection pressure, low product quality, and high production cost when preparing α-amylase inhibitors.

Method used

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  • Method for extracting alpha-diastase restrainer from phaseolus limensis

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Experimental program
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Effect test

Embodiment 1

[0023] Weigh 100 grams of pulverized kidney bean flour with a balance and add it to 100 grams of buffer solution with a pH of 2.0, stir evenly, extract in an ultrasonic extraction device with a power of 50W and a frequency of 20KHz for 5 minutes, filter to obtain the filtrate, and use hot water to obtain the filtrate. The exchange method conventionally heats for 30 minutes to eliminate enzymes, centrifuges for 30 minutes and takes the supernatant, dialyzes in deionized water for 3 hours, adds organic alcohol to the extracted solution after dialysis to obtain a precipitate, and the volume of the extracted solution and organic alcohol The ratio is 5:1, and after being dried under normal pressure, an α-amylase inhibitor product with a protein content of 80.3% and a biological activity of 5000U / g is obtained.

Embodiment 2

[0025] Weigh 100 grams of pulverized kidney bean powder with a balance and add it to 500 grams of buffer solution with a pH of 3.0, stir evenly, extract in an ultrasonic extraction device with a power of 100W and a frequency of 40KHz for 10 minutes, filter to obtain the filtrate, and use heat The exchange method conventionally heats for 30 minutes to eliminate enzymes, centrifuges for 30 minutes and takes the supernatant, dialyzes in deionized water for 3 hours, adds organic alcohol to the extracted solution after dialysis to obtain a precipitate, and the volume of the extracted solution and organic alcohol The ratio is 5:1, and after drying under reduced pressure, an α-amylase inhibitor product with a protein content of 83.6% and a biological activity of 7192U / g is obtained.

Embodiment 3

[0027] Weigh 100 grams of pulverized kidney bean flour with a balance and add it to 1000 grams of buffer solution with a pH of 4.0, stir evenly, extract in an ultrasonic extraction equipment with a power of 200W and a frequency of 60KHz for 30 minutes, filter to obtain the filtrate, and use heat The exchange method conventionally heats for 30 minutes to eliminate enzymes, centrifuges for 30 minutes and takes the supernatant, dialyzes in deionized water for 3 hours, adds organic alcohol to the extracted solution after dialysis to obtain a precipitate, and the volume of the extracted solution and organic alcohol The ratio is 5:1, and after drying under normal pressure, an α-amylase inhibitor product with a protein content of 89.1% and a biological activity of 8931U / g is obtained.

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Abstract

The invention discloses a method of extracting Alpha amylase inhibitor from kidney beans. The method is as follows: the crushed kidney beans are first dispersed in a buffer solution with a definite pH value, and then shifted into an ultrasonic device for ultrasonic extraction, and then conducted enzyme elimination, centrifugalization, dialysis and ethanol sediment, dried and finally the Alpha amylase inhibitor is obtained. The Alpha amylase inhibitor extracted from kidney beans in the invention is a white solid powder with a protein content of 80 to 95 percent and an inhibitory activity of 5,000 to 50,000 U/g. Compared with the prior method of lixiviation and extraction, the method of extracting Alpha amylase inhibitor from kidney beans has the characteristics of high speed, high efficiency, energy conservation and small pollution. The one-time extracted Alpha amylase inhibitor has high content and strong activity, thereby avoiding the fact that circulative extractions consume a large quantity of water and energy. The method of extracting Alpha amylase inhibitor from kidney beans is a novel green environment-friendly technology with a wide range of application prospects.

Description

technical field [0001] The invention relates to a method for extracting alpha-amylase inhibitors, in particular to a method for extracting alpha-amylase inhibitors from kidney beans. Background technique [0002] Obesity has become a major obstacle to the progress and civilization of human society, and it is also a class of diseases that endanger human health. It will not only induce the early arrival of symptoms such as diabetes and hyperlipidemia, but also bring a huge mental burden to the patient himself. Therefore, the prevention and treatment of obesity has become one of the issues worthy of human attention, and exploring new prevention and treatment measures is one of the key points and difficulties of scientific research. [0003] At present, the methods for preventing and treating obesity include exercise to burn fat, liposuction, dieting and taking health care products. Exercise method is currently the most environmentally friendly method, but because it either ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P3/04C07K14/415
Inventor 贾光锋贾荣博周雯丽程全贤
Owner SHAANXI ZHUORUI BIOTECH
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