Method for jointly treating stalks by steam explosion and microorganism fermentation

A microbial fermentation and combined treatment technology, applied in animal feed, animal feed, application, etc., can solve the problems of large-scale production that is not suitable for industrialization, low biotransformation efficiency, and increased recycling costs, so as to alleviate food shortages and solve The effect of the competition between humans and animals for food

Active Publication Date: 2011-06-01
河南德邻生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological pretreatment method has been paid more and more attention because it can improve the utilization efficiency of straw without polluting the environment, but it also has disadvantages: the biological conversion efficiency is low
However, there are few studies on the application of straw blasting technology to animal feed production at home and abroad.
Alkali pretreatment can be carried out at room temperature, and can effectively reduce the lignin content in the straw, but it will cause the loss of part of the fermentable sugar during the treatment
Acid treatment can degrade hemicellulose in lignocellulosic raw materials, making cellulose more easily utilized by microorganisms, but the high concentration of acid and alkali increases the cost of recycling in actual production, and is not suitable for large-scale industrial production

Method used

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  • Method for jointly treating stalks by steam explosion and microorganism fermentation
  • Method for jointly treating stalks by steam explosion and microorganism fermentation
  • Method for jointly treating stalks by steam explosion and microorganism fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 (taking corn stalks as an example, the method of the present invention is described in detail)

[0026] A method for combined treatment of stalks by steam explosion and microbial fermentation, comprising the steps of:

[0027] ① Straw steam explosion pretreatment: The corn straw crushed through a 20-mesh sieve with a moisture content of 10wt% was subjected to steam explosion treatment under the condition of a steam pressure of 2.5 Mpa, kept for 200 s, and the treated straw was naturally air-dried to moisture content. Ratio ≤ 10wt% to get blasting straw, spare;

[0028] ②Preparation of Aspergillus oryzae seed solution: Inoculate Aspergillus oryzae into a petri dish made of PDA medium, culture it statically at 30°C for 3 days, wash the petri dish with sterilized saline after the spores mature, and transfer the spores in the petri dish Bacteria triangular flask, the preparation concentration is 1×10 6 The spore suspension per mL is the spore seed solution; t...

Embodiment 2

[0031] With reference to Example 1, the difference is that Trichoderma konshii is used to replace Aspergillus oryzae for the fermentation test.

Embodiment 3

[0033] A method for combined treatment of stalks by steam explosion and microbial fermentation, comprising the steps of:

[0034] ①Steam explosion pretreatment of straw: The straw crushed through a 10-mesh sieve with a moisture content of 15wt% was kept under a steam pressure of 1.8MPa for 240s and then released for steam explosion treatment, and then dried naturally until the moisture content was 15wt% blasting straw, spare;

[0035] ②Preparation of Trichoderma konshii seed solution: inoculate Trichoderma konii into a petri dish made of PDA medium, culture at 28°C for 5 days, rinse the petri dish with sterilized physiological saline after the spores mature, and put the Transfer the spores to a sterilized Erlenmeyer flask, and prepare a concentration of 1×10 8 The spore suspension per mL is the spore seed liquid; the PDA medium was pre-sterilized at 121°C and 0.15MPa pressure for 15 minutes, and then made into a culture medium containing 15 mL of PDA medium under aseptic cond...

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PUM

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Abstract

The invention relates to a method for jointly treating stalks by steam explosion and microorganism fermentation, comprising the following steps of: firstly, carrying out steam explosion pretreatment on the stalks to obtain exploded stalks; then preparing spore seed liquid: inoculating aspergillus oryzae or trichoderma Koningi to a PDA (Potato Dextrose Agar) culture medium, standing and culturing for 3-5 days at the temperature of 28-32 DEG C, and preparing the spore seed liquid with the concentration of 106-108 per mL; and finally carrying out microorganism fermentation: uniformly mixing 18g of exploded stalks, 2g of bran and 30mL of mineral element nutrient solution, adjusting pH to be 7.0 with Ca(OH)2, sterilizing for 15min at the temperature of 121 DEG C and the pressure of 0.15MPa to obtain a solid fermentation medium a, inoculating the spore seed liquid according to 2-4% of inoculum size, and culturing the spore seed liquid for 5-7 days at the temperature of 28-32 DEG C. The fermentation stalks obtained by utilizing the method have low content of lignose, cellulose and hemicellulose and high activity of filter paper carbohydrase, CMC (carboxymethyl cellulose) enzyme, amylase and protease.

Description

technical field [0001] The invention relates to a method for processing straw, in particular to a method for combined treatment of straw by steam explosion and microbial fermentation. Background technique [0002] A large number of studies at home and abroad have confirmed that using biotechnology to fully tap and utilize the potential value of crop straw is one of the most effective means to alleviate the shortage of global food resources. According to preliminary estimates, if 10-20% of bio-fermented straw is used to replace cereal grains for pig production, the country can save nearly 30-50 million tons of grain every year, which can completely solve the problem of 30 million tons of grain grains in my country every year. The problem of the shortage of feed grains has particularly important social and economic significance. [0003] The main components of straw are crude fibers, including cellulose, hemicellulose and lignin, and the high degree of polymerization and cryst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K10/37
CPCY02P60/87
Inventor 尹清强常娟郑秋红左瑞雨刘俊熙卢敏郭宏伟
Owner 河南德邻生物制品有限公司
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