Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent
A technology of surfactant and bamboo leaf flavonoids, applied in food ingredients as antioxidants, food science, food preservation, etc., can solve the problems of high cost, low efficiency, large solvent consumption, etc., achieve low equipment requirements, solve consumption The effect of large volume and short extraction time
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Embodiment 1
[0024] A method for ultrasonic-surfactant synergistically extracting bamboo leaf flavonoids, comprising the steps of: putting 2.0g of bamboo leaf powder into an Erlenmeyer flask with a stopper, adding 95% (Quality) ethanol, add extracting liquid (extracting liquid is the sodium lauryl sulfate aqueous solution of 2.5 times of critical micelle concentration (cmc)) by liquid-solid ratio 20:1 (mL / g); Under the condition of normal temperature, ultrasonic-surfactant synergistic extraction was carried out twice with ultrasonic power 400W, each extraction time was 20min, suction filtration, combined filtrate, constant volume to 100mL, using the standard curve method to calculate the yield of bamboo leaf flavonoids was 1.88 %.
Embodiment 2
[0026] A method for ultrasonic-surfactant synergistically extracting bamboo leaf flavonoids, comprising the steps of: putting 2.0g of bamboo leaf powder into an Erlenmeyer flask with a stopper, adding 95% (Quality) ethanol, add extracting liquid (extracting liquid is the sodium lauryl sulfate aqueous solution of 2.5 times of critical micelle concentration (cmc)) by liquid-solid ratio 20:1 (mL / g); Under the condition of normal temperature, ultrasonic-surfactant synergistic extraction was carried out twice with ultrasonic power 350W, each extraction time was 20min, suction filtration, combined filtrate, constant volume to 100mL, using the standard curve method to calculate the yield of bamboo leaf flavonoids was 1.70 %.
Embodiment 3
[0028] A method for ultrasonic-surfactant synergistically extracting bamboo leaf flavonoids, comprising the steps of: putting 2.0g of bamboo leaf powder into an Erlenmeyer flask with a stopper, adding 95% (Quality) ethanol, add extracting liquid (extracting liquid is the sodium lauryl sulfate aqueous solution of 2.5 times of critical micelle concentration (cmc)) by liquid-solid ratio 20:1 (mL / g); Under the condition of normal temperature, ultrasonic-surfactant synergistic extraction was carried out twice with ultrasonic power 450W, each extraction time was 20min, suction filtration, combined filtrate, constant volume to 100mL, the yield of bamboo leaf flavonoids calculated by the standard curve method was 1.78 %.
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