Liver cell membrane bionic liposome drug carrier as well as preparation method and application thereof

A cell membrane, biomimetic lipid technology, applied in liposome delivery, drug combination, drug delivery, etc., can solve the problems of non-specific site sequence cutting trouble, low off-target rate, off-target effect, etc.

Inactive Publication Date: 2016-11-16
李因传
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  • Claims
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Problems solved by technology

Both have their own advantages and disadvantages. For example, the off-target rate of TALEN technology is very low, and the DNA element limited to targeting is relatively large, which exceeds the capacity of most vectors, so it cannot be used for multiple targeting; while CRISPR technology only uses a nuclease CAS9, carrying Multiple gRNAs ca...

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  • Liver cell membrane bionic liposome drug carrier as well as preparation method and application thereof
  • Liver cell membrane bionic liposome drug carrier as well as preparation method and application thereof
  • Liver cell membrane bionic liposome drug carrier as well as preparation method and application thereof

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Embodiment Construction

[0032] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0033] 1. Immortalization of primary hepatocytes

[0034] The present invention uses the traditional cell immortalization method to process cells. The hepatocytes come from autologous hepatocytes, or healthy volunteer hepatocytes, preferably healthy volunteer hepatocytes, without hepatitis virus carrying; mainly hTERT, SV40 large T antigen, Epstein-Barr virus, c-MYC and other methods of treatment, the individual treatment and / or combined application of the above methods. The combined treatment of hTERT and SV40 large T antigen is preferred, and the expression plasmids of hTERT and SV40 large T antigen are respectively encoded by a plasmid or encoded by a single plasmid. In the present invention, hTERT (GenBank: NM_198253 or NM_001193376) and SV...

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Abstract

The invention relates to a liver cell membrane bionic liposome drug carrier, a preparation method of the liver cell membrane bionic liposome drug carrier, and application of the liver cell membrane bionic liposome drug carrier. The liver cell membrane bionic liposome drug carrier is characterized in that (1) the liposome drug carrier has a cell membrane protein component; (2) the liposome drug carrier is capable of loading drug in vitro, and is used for cell targeting fusion release; (3) the cell membrane protein component of the liposome comes from immortalized human liver cells, and is used for conveying targeted shear plasmids of a hepatitis virus genome; (4) the cell membrane protein component of the liposome comes from an immortalized liver tumor cell line, and is used for targeted drug delivery of liver tumor. The liver cell membrane bionic liposome drug carrier can be used for in vivo targeted hepatic cell transmission and drug delivery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) gene targeting plasmids.

Description

technical field [0001] The invention belongs to the technical field of cell therapy and precise delivery of liposome drugs, and relates to a liver cell membrane bionic liposome drug carrier, a preparation method of the liver cell membrane bionic liposome drug carrier, and a liver cell membrane bionic liposome drug carrier. The application of cell membrane biomimetic liposome drug carrier can be used for drug delivery and treatment precisely targeting the liver. Background technique [0002] The targeted delivery system of drugs can reduce the dosage of drugs, improve the utilization rate of drugs, reduce the non-targeted toxic and side effects of drugs, and increase the effectiveness of drugs. Current targeted drug delivery systems include liposomes, emulsions, microspheres, nanoparticles, etc., wherein the liposome drug delivery system is loaded with drugs in ultramicrospherical vesicles encapsulated by lipid molecules (liposomes) Among them, hydrophilic drugs can be loade...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K48/00A61K41/00A61K31/7088A61K47/42A61P35/00
CPCA61K9/1271A61K9/0009A61K31/7088A61K41/00A61K47/42
Inventor 李因传
Owner 李因传
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