Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lotus japonicus ERF transcription factors as well as encoding gent, expression vector and application thereof

A technology of transcription factors and japonicus japonicus, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem that the fundamental improvement of plant stress resistance cannot be achieved

Inactive Publication Date: 2015-01-21
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made in the research of plant stress resistance genetic engineering, the focus is on the introduction of individual functional genes to improve a certain resistance, so that the comprehensive and fundamental improvement of plant stress resistance cannot be achieved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lotus japonicus ERF transcription factors as well as encoding gent, expression vector and application thereof
  • Lotus japonicus ERF transcription factors as well as encoding gent, expression vector and application thereof
  • Lotus japonicus ERF transcription factors as well as encoding gent, expression vector and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Isolation, Identification and Cloning of LcERF053 Transcription Factor of Lotus japonicus

[0062] 1 Materials and methods

[0063] 1.1 Materials

[0064] 1.1.1 Plant material cultivation and stress treatment

[0065] Using japonicus-"Leo" as the material, select mature, full-bodied seeds that are free from diseases and insect pests, soak them in 0.1% mercuric chloride for 15-20 minutes, rinse them with sterile water for 3 times, dry them, and place the sterilized seeds in B5 for cultivation On the basis, they were cultured in a light incubator for 30 days, treated with 150mM sodium chloride for 12h and 24h, and the leaves were collected and immediately frozen in liquid nitrogen, and stored in a -80°C refrigerator for later use.

[0066] 1.1.2 Strains and plasmids

[0067] Escherichia coli (Escherichia coli): DH5α preserved in the inventor's laboratory

[0068] PMD19-T cloning vector was purchased from TaKaRa Biological Company

[0069] 1.2 Experimental m...

Embodiment 2

[0109] Example 2 Construction of a plant expression vector containing the LcERF053 gene and its functional verification in Arabidopsis

[0110] 1 Materials and methods

[0111] 1.1 Materials

[0112] 1.1.1 Plant material

[0113] Arabidopsis: Columbia type, preserved by the inventor's laboratory.

[0114] 1.1.2 Strain and carrier

[0115] Escherichia coli (Escherichia coli) DH5α was preserved by the inventor's laboratory; Agrobacterium tumefaciens strain LBA4404 was preserved by the inventor's laboratory; pMD19-T cloning vector was purchased from TaKaRa; gateway entry vector pDNOR201 and expression vector pH7WG2D were purchased from invitrogen.

[0116] 1.1.3 Reagents

[0117] Various restriction endonucleases, rTaq enzymes, and ExTaq enzymes were purchased from Takara; T4 DNA ligase was purchased from Invitrogen; Taq enzymes were purchased from Beijing Quanshijin Biotechnology Co., Ltd.; Gel / PCR Extraction Kit was purchased from BioMIGA.

[0118] 1.1.4 Medium

[0119] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a lotus japonicus ERF transcription factors as well as an encoding gent, an expression vector and application thereof. The ERF transcription factor cDNA is obtained by isolating from lotus japonicus and has a nucleotide sequence as shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein is as shown in SEQ ID No. 2. The invention also provides the expression vector and a host cell containing the transcription factor cDNA. Functional analysis experiments show that by overexpressing ERF transcription factor cDNA in plants, the resistance of plants against adversity stress comprising high salt, drought, low temperature and the like can be effectively enhanced or improved. The invention further provides the application of the ERF transcription factors in improvement on adversity stress resistance of plants or breeding of new varieties of adversity-stress-resistant transgenic plants. The application is achieved by the following steps: constructing a recombinant plant expression vector containing the ERF transcription factor cDNA; transforming the recombinant plant expression vector into cells of the plants, and breeding and screening to obtain the transgenic plants with improved adversity stress resistance.

Description

technical field [0001] The present invention relates to transcription factors, in particular to the stress-resistance-related ERF transcription factors isolated from Lotus corniculatus L. and their coding genes, and the present invention further relates to their application in improving the stress resistance of plants The invention belongs to the field of ERF transcription factor and its application. Background technique [0002] Soil drought and salinization seriously hinder the growth of crops and reduce the yield of crops, which have become important factors restricting the sustainable development of irrigated agriculture in the world and affecting the ecological environment. With the increase of population, the reduction of cultivated land and the scarcity of water resources, it is of extremely important practical significance to improve the efficiency of water resource utilization and fully develop and utilize saline-alkali land. The improvement of saline-alkali land i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00
Inventor 吴燕民李诗刚孙占敏周美亮李金博
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com