Method for identifying glyphosate resistance of cotton
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An identification method and glyphosate-resistant technology are applied in the field of identification of cotton resistance/resistance to glyphosate, and can solve problems such as affecting the use of glyphosate, non-standard equipment for glyphosate, and non-standard pharmaceuticals.
Inactive Publication Date: 2011-08-31
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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However, there are currently the following problems in the identification of cotton's resistance to glyphosate: the pesticides used are not standardized, the application period and dosage of glyphosate are not clear, the equipment for spraying glyphosate is not standardized, and the evaluation standards are not uniform
The above problems lead to large systematic errors in the identification results, which affect the use of glyphosate in the subsequent breeding work and variety promotion.
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Embodiment 1
[0023] Choose a polyethylene plastic bowl with a diameter of 15cm and a height of 10cm, and drill three 0.5cm diameter permeable holes at the bottom of the bowl; cover the permeable holes with a single layer of filter paper; put it into the culture medium of raw soil: yellow sand: Kaiyin nutrient soil 1:1:1, Water from the bottom of the container to saturate the soil.
[0024] The accelerated germination transgenic glyphosate-resistant cotton MON88913, the recipient cotton variety Coker312 and the conventional variety Jimian 998 were sown respectively, with 5 seeds per pot. Cover with the same substrate 1 cm after sowing. After the emergence of cotton seedlings, 2 plants were kept in each pot.
[0025] When the cotton grows to the 3-4, 6-8 and 9-10 true leaf stages, put the spray tower with a movable 8002 fan-shaped nozzle with a pressure of 2.75Kpa, and spray the herbicide Roundup (Roundup) water agent; glyphosate The concentrations were 0g ai / mu, 61.5g ai / mu, 82g ai / mu, 12...
Embodiment 2
[0035] 1. Select cotton materials MON88913, Coker312, Jimian 998 and Jimian 20 for standby. Delineate 162 30m in the field 2 Cotton was planted in wide and narrow rows, the wide row spacing was 90 cm, the narrow row spacing was 45 cm, the density was 4000 plants / mu, hole sowing, and 4 seeds were sown in each hole. After the emergence of cotton, 1 plant is left in each hole for thinning; 54 plots are sown for each of the above-mentioned cotton materials;
[0036] 2. When the above-mentioned cotton materials grow to 3, 6 and 9 true leaf stages, use compressed CO with a pressure-limiting valve and 8001-8003 fan-shaped nozzles respectively. 2 The plot sprayer sprays the herbicide Roundup (Roundup) water agent; wherein, "compressed CO 2 Community sprayer" uses compressed CO 2 As an air source, a field test plot spraying device that sprays pesticides.
[0037] 3. The concentrations of Roundup are 0g ai / mu, 61.5g ai / mu, 82g ai / mu, 123g ai / mu, 164g ai / mu and 246g ai / mu respectively,...
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Abstract
The invention relates to a method for identifying the glyphosate resistance of cotton, and the method is used for identifying the glyphosate resistance / tolerance of cotton materials. The method comprises the following steps: seeding cotton seeds in a container or a field; after a certain leaf age of grown cotton, respectively spraying 61.5ai / mu-82g ai / mu or 123g ai / mu-246g ai / mu phytocide glyphosate; and recording the death rate of the cotton, and recording the yield properties while harvesting, wherein the cotton which is sprayed with the 61.5g ai / mu-82g ai / mu glyphosate and dies is sensitive to the glyphosate, the cotton which is sprayed with 123g ai / mu-246g ai / mu glyphosate, does not die and achieves no yield reduction is resistant to the glyphosate, and the cotton which is sprayed with 123g ai / mu-246g ai / mu glyphosate, does not die and achieves yield reduction is tolerant to the glyphosate. The method provided by the invention is suitable for identifying and evaluating the glyphosate resistance / tolerance of cottons of routine variety, hybrid variety and transgenic glyphosate-resistant variety, can be used for accurately measuring the glyphosate resistance / tolerance of the cotton and has strong practicability.
Description
technical field [0001] The invention relates to the technical field of plant protection, in particular to a glyphosate-resistant identification method for cotton. Background technique [0002] Weeds are one of the limiting factors for stable cotton production. The area of weed damage in cotton fields in my country is more than 70 million mu every year, and the loss of production reduction is more than 12.5 billion yuan. In the five major cotton regions in my country, there are more than 60 kinds of dominant weeds, and the main harmful species are crabgrass, goosegrass, barnyardgrass, foxtail, daughter of a daughter, reed, amaranth, purslane, velvetleaf, and amaranth , quinoa, mother-in-law, snakehead sausage, spinach, lettuce, endive, Mengshan lettuce, field bindweed, Cyperus rotundum, etc. Weed control in cotton fields is mainly chemical control, mainly by spraying soil sealing herbicides acetochlor and metolachlor after sowing and pre-emergence spraying or spraying sele...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06
Inventor 李香菊梁革梅崔海兰吴孔明
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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