New method for increasing seed reproduction yield of rice intelligent sterile line

Abstract

The invention provides a new method for increasing the seed reproduction yield of a rice intelligent sterile line. The new method comprises the following steps: (1) constructing the dominant mutant gene of EPSPS gene, the fertility restorer gene and the pollen inactivation gene of the rice recessive male nuclear sterile gene and a gene for enabling the rice to be sensitive to another type of herbicides in the same T-DNA zone of a vector to form linked genes, and then importing the linked genes into a corresponding sterile line by use of the agrobacterium-mediated transformation technology to form the intelligent sterile line; and (2) carrying out selfing on the intelligent sterile line, and in the process of breeding the intelligent sterile line by use of the group, spraying herbicide glyphosate to remove the sterile plants so as to obtain a consistent fertile plant group. Therefore, the seed reproduction yield of the intelligent sterile line is increased by 1 time in contrast with the seed reproduction yield when the herbicide glyphosate is not applied; besides, the new method has the advantages that sterile and fertile seeds can be screened more accurately and reliably compared with a color sorting method, and is convenient to use.

Description

technical field [0001] The invention relates to a new method for improving the seed reproduction yield of rice intelligent sterile lines by utilizing resistance to herbicides as an accurate and efficient screening marker for fertile plants of intelligent sterile lines of rice, and belongs to the field of cultivation of intelligent sterile lines . Background technique [0002] The breeding of intelligent sterile lines is the product of the combination of modern biotechnology and traditional breeding methods. It not only makes it possible to use different types of rice recessive male sterility genes, but also realizes extensive matching and screening of strong dominant combinations . However, the currently reported methods (such as color sorting) as screening markers for intelligent sterile lines of rice have great defects in practical application, because the rice hulls are thick and color sorting is difficult. [0003] The successful utilization of rice heterosis depends o...

AI Technical Summary

Problems solved by technology

However, this method has great defects in practical application as a rice intelligent CMS screening marker, because the rice husk is thick and color selection is difficult.