Plant endophytic fungus Epicoccum nigrum FZT214 and application thereof

A plant endophytic fungus, FZT214 technology, applied in the field of microorganisms, can solve the problems of slow growth, long growth cycle, low biomass and so on

Active Publication Date: 2021-02-12
KUNMING UNIV OF SCI & TECH
View PDF25 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slow growth of most hyperaccumulative plants, low biomass, long growth cycle, and specificity for heavy metal accumulation, some poisoning symptoms will appear whe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant endophytic fungus Epicoccum nigrum FZT214 and application thereof
  • Plant endophytic fungus Epicoccum nigrum FZT214 and application thereof
  • Plant endophytic fungus Epicoccum nigrum FZT214 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Isolation, screening and identification of Eperococcus nigricans FZT214

[0032] A. Collect the plant seed samples of the dominant plant Nepeta nepeta in the slag area of ​​the heavy metal polluted land, and rinse them under tap water;

[0033]B. Randomly select 50 seeds from Nepeta plant samples, rinse them under tap water, and carry out surface disinfection according to the following procedures: rinse with 75% ethanol for 2 minutes, rinse with sterile water for 3 times; available chlorine concentration 5 Rinse with % NaClO solution for 2 min, rinse with sterile water for 3 times, and place on sterile filter paper to absorb the moisture. Paste the surface-sterilized seeds on a PDA plate (90mm), 5 seeds per plate, culture at 25°C for 60 days, and observe the next day. If fungi grow around the tissue block during the culture period, pick, purify and store it. At the same time, Use the rinsing solution test method to check whether the seed surface is thorou...

Embodiment 2

[0039] Example 2: Effects of plant endophytic fungus Eperococcus niger FZT214 on germination of Nepeta nepeta seeds under heavy metal Cd stress

[0040] 1. Preparation of E. niger FZT214 suspension: Select the previously preserved pure and pollution-free FZT214 strain and inoculate it into PDA medium for activation, and place it in a water-proof constant temperature incubator at 28°C for 7 days. Select a good growth and no pollution Pick the mycelium and inoculate it into the PDB medium, and cultivate it in a constant temperature shaker at 28°C and 130rpm for 3-5 days. Rinse 3 times to prevent the mycelium from being stained with the medium, then cut it into pieces with sterile scissors, transfer the cut up mycelia to sterile water and dilute to 150 mL to make a suspension of FZT214 mycelia;

[0041] 2. Treat the seeds of Nepeta nepeta with mycelia suspension of E. niger FZT214 for 1 h, and treat the sterile water as the control group. Soak the infected seeds and control seeds...

Embodiment 3

[0043] Embodiment 3: Under the stress of heavy metal Cd, the influence of Eperococcus nigricans FZT214 on the growth period Nepeta nepeta

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an Epicoccum nigrum FZT214. The preservation number of the Epicoccum nigrum FZT214 in the China General Microbiological Culture Collection Center is CGMCC No. 21045. The strainis applied to promotion of plant seed germination and seedling growth, and experiments prove that the strain can promote the plant seed germination and seedling growth, increase the plant seed yieldand enhance the accumulation capacity for heavy metal cadmium under the stress of heavy metal pollution, and is suitable for in-situ remediation of large-area heavy metal cadmium polluted soil Cd.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a plant endophytic fungus Epicoccus niger ( Epicoccum nigrum ) FZT214 and its application in promoting plant seed germination and seedling growth under heavy metal stress. Background technique [0002] Due to human activities such as mining and smelting, extensive use of chemical fertilizers and pesticides, farmland sewage irrigation, and solid waste disposal, soil heavy metal pollution is becoming more and more serious. Excessive heavy metals in soil will not only inhibit crop growth, but also damage the structure of soil microbial flora. It will also produce toxic amplification through the food chain, seriously threatening human health, and the remediation of contaminated soil has become one of the hot issues in environmental research. Among all remediation methods, phytoremediation (Phytomediation) shows a good application prospect in the remediation of h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14A01N63/30A01P21/00B09C1/10C12R1/645
CPCA01N63/30B09C1/105C12R2001/645C12N1/145Y02A40/10
Inventor 李海燕李绍仕汤雯婷毛文沁江悦娟
Owner KUNMING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap