Plant endophytic fungus Epicoccum nigrum FZT214 and application thereof
A plant endophytic fungus, FZT214 technology, applied in the field of microorganisms, can solve the problems of slow growth, long growth cycle, low biomass and so on
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Embodiment 1
[0031] Embodiment 1: Isolation, screening and identification of Eperococcus nigricans FZT214
[0032] A. Collect the plant seed samples of the dominant plant Nepeta nepeta in the slag area of the heavy metal polluted land, and rinse them under tap water;
[0033]B. Randomly select 50 seeds from Nepeta plant samples, rinse them under tap water, and carry out surface disinfection according to the following procedures: rinse with 75% ethanol for 2 minutes, rinse with sterile water for 3 times; available chlorine concentration 5 Rinse with % NaClO solution for 2 min, rinse with sterile water for 3 times, and place on sterile filter paper to absorb the moisture. Paste the surface-sterilized seeds on a PDA plate (90mm), 5 seeds per plate, culture at 25°C for 60 days, and observe the next day. If fungi grow around the tissue block during the culture period, pick, purify and store it. At the same time, Use the rinsing solution test method to check whether the seed surface is thorou...
Embodiment 2
[0039] Example 2: Effects of plant endophytic fungus Eperococcus niger FZT214 on germination of Nepeta nepeta seeds under heavy metal Cd stress
[0040] 1. Preparation of E. niger FZT214 suspension: Select the previously preserved pure and pollution-free FZT214 strain and inoculate it into PDA medium for activation, and place it in a water-proof constant temperature incubator at 28°C for 7 days. Select a good growth and no pollution Pick the mycelium and inoculate it into the PDB medium, and cultivate it in a constant temperature shaker at 28°C and 130rpm for 3-5 days. Rinse 3 times to prevent the mycelium from being stained with the medium, then cut it into pieces with sterile scissors, transfer the cut up mycelia to sterile water and dilute to 150 mL to make a suspension of FZT214 mycelia;
[0041] 2. Treat the seeds of Nepeta nepeta with mycelia suspension of E. niger FZT214 for 1 h, and treat the sterile water as the control group. Soak the infected seeds and control seeds...
Embodiment 3
[0043] Embodiment 3: Under the stress of heavy metal Cd, the influence of Eperococcus nigricans FZT214 on the growth period Nepeta nepeta
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